Q5RFH3 (NDKB_PONAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoside diphosphate kinase B
Short name=NDK B
Short name=NDP kinase B
EC=2.7.4.6

Alternative name(s):
Histidine protein kinase NDKB
EC=2.7.13.3

Gene names

Name:

NME2

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	152 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Negatively regulates Rho activity by interacting with AKAP13/LBC. Exhibits histidine protein kinase activity By similarity.
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate. ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Cofactor	Magnesium By similarity.
Subunit structure	Hexamer of two different chains: A and B (A6, A5B, A4B2, A3B3, A2B4, AB5, B6). Interacts with AKAP13 and CAPN8 By similarity.
Subcellular location	Cytoplasm. Cell membrane By similarity.
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cell membrane Cytoplasm Membrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: InterPro GTP biosynthetic process Inferred from electronic annotation. Source: InterPro UTP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: EC protein histidine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 152	151	Nucleoside diphosphate kinase B		PRO_0000250202
Regions
	Region	2 – 66	65	Interaction with AKAP13 By similarity
Sites
	Active site	118	1	Pros-phosphohistidine intermediate By similarity
	Binding site	12	1	ATP By similarity
	Binding site	60	1	ATP By similarity
	Binding site	88	1	ATP By similarity
	Binding site	94	1	ATP By similarity
	Binding site	105	1	ATP By similarity
	Binding site	115	1	ATP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	12	1	N6-acetyllysine By similarity
	Modified residue	49	1	N6-acetyllysine By similarity
	Modified residue	52	1	Phosphotyrosine By similarity
	Modified residue	56	1	N6-acetyllysine By similarity
	Modified residue	85	1	N6-acetyllysine By similarity
	Modified residue	94	1	Phosphothreonine By similarity
	Modified residue	100	1	N6-acetyllysine By similarity
	Modified residue	124	1	N6-acetyllysine By similarity
	Modified residue	128	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5RFH3 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 8D2D9361C94DC938	FASTA	152	17,300
	10 20 30 40 50 60 MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYTDLKDRPF 70 80 90 100 110 120 FPGLVKYMNS GPVVAMVWEG LNVVKTGRLM LGETNPADSK PGTIRGDFCI QVGRNIIHGS 130 140 150 DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR857184 mRNA. Translation: CAH89484.1.
RefSeq	NP_001127154.1. NM_001133682.1.
UniGene	Pab.9662.
3D structure databases
HSSP	HSSP built from PDB template 1NUE based on UniProtKB P22392.
ProteinModelPortal	Q5RFH3.
SMR	Q5RFH3. Positions 2-152.
ModBase	Search...
Proteomic databases
PRIDE	Q5RFH3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100174205.
KEGG	pon:100174205.
Organism-specific databases
CTD	4831.
Phylogenomic databases
HOVERGEN	HBG000423.
InParanoid	Q5RFH3.
Family and domain databases
InterPro	IPR001564. Nucleoside_diP_kinase. IPR023005. Nucleoside_diP_kinase_AS. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 1 hit.
KO	K00940.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
SMART	SM00562. NDK. 1 hit. [Graphical view]
SUPFAM	SSF54919. NDK. 1 hit.
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDKB_PONAB

Accession

Primary (citable) accession number: Q5RFH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	December 21, 2004
Last modified:	November 16, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program