Q5RFH1 (TWF2_PONAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Twinfilin-2

Gene names

Name:

TWF2

Organism

Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) [Reference proteome]

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Ponginae › Pongo

Protein attributes

Sequence length	341 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. May play a role in regulating the mature length of the middle and short rows of stereocilia By similarity.
Subunit structure	Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF1 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down-regulated. Interacts with MYO7A By similarity.
Subcellular location	Cytoplasm › cytoskeleton By similarity. Cytoplasm › perinuclear region By similarity. Cell projection › stereocilium By similarity. Note: Perinuclear and G-actin-rich cortical actin structure sublocalization By similarity.
Post-translational modification	Phosphorylated on both serine and threonine residues By similarity.
Sequence similarities	Belongs to the actin-binding proteins ADF family. Twinfilin subfamily. Contains 2 ADF-H domains.

Ontologies

Keywords
Biological process	Cilium biogenesis/degradation
Cellular component	Cell projection Cytoplasm Cytoskeleton
Domain	Repeat
Ligand	Actin-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell projection organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell stereocilium Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 341	›341	Twinfilin-2		PRO_0000233138
Regions
	Domain	‹1 – 131	›131	ADF-H 1
	Domain	169 – 305	137	ADF-H 2
Amino acid modifications
	Modified residue	6	1	N6-acetyllysine By similarity
	Modified residue	11	1	N6-acetyllysine By similarity
	Modified residue	301	1	Phosphotyrosine By similarity
	Modified residue	341	1	Phosphoserine By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q5RFH1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 98C5E459EB3413A5	FASTA	341	38,707
	10 20 30 40 50 60 ATEELKEFFA KARAGSVRLI KVVIEDEQLV LGASQEPVGR WDRDYDRAVL PLLDAQQPCY 70 80 90 100 110 120 LLYRLDSQNA QGFEWLFLAW SPDNSPVRLK MLYAATRATV KKEFGGGHIK DELFGTVKDD 130 140 150 160 170 180 LSFAGYQKHL SSCAAPAPLT SAERELQQIR INEVKTEISV ESKHQTLQGL AFPLQPEAQR 190 200 210 220 230 240 ALQQLKQKMV NYIQMKLDLE RETIELVHTE STDVAQLPSR VPRDAARYHF FLYKHTHEGD 250 260 270 280 290 300 LLESVVFIYS MPGYKCSIEE RMLYSSCKSR LLDSVEQDFH LEIAKKIEIG DGAELTAEFL 310 320 330 340 YDEVHPKQHA FKQAFAKPKG PGGKRGHKRL IRGPGENGDD S

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.

Web resources

Protein Spotlight

Molecular embrace - Issue 73 of August 2006

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR857187 Transcribed RNA. Translation: CAH89486.1.
3D structure databases
ProteinModelPortal	Q5RFH1.
SMR	Q5RFH1. Positions 1-129, 153-305.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG000848.
InParanoid	Q5RFH1.
Family and domain databases
InterPro	IPR002108. Actin-bd_cofilin/tropomyosin. [Graphical view]
Pfam	PF00241. Cofilin_ADF. 2 hits. [Graphical view]
SMART	SM00102. ADF. 2 hits. [Graphical view]
PROSITE	PS51263. ADF_H. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TWF2_PONAB

Accession

Primary (citable) accession number: Q5RFH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	December 21, 2004
Last modified:	October 3, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program