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Q5RFH1 (TWF2_PONAB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein attributes

Sequence length341 AA.
Sequence statusFragment.
Protein existenceInferred from homology

General annotation (Comments)

Function

Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. May play a role in regulating the mature length of the middle and short rows of stereocilia By similarity.

Subunit structure

Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF1 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down-regulated. Interacts with MYO7A By similarity.

Subcellular location

Cytoplasmcytoskeleton By similarity. Cytoplasmperinuclear region By similarity. Cell projectionstereocilium By similarity. Note: Perinuclear and G-actin-rich cortical actin structure sublocalization By similarity.

Post-translational modification

Phosphorylated on both serine and threonine residues By similarity.

Sequence similarities

Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.

Contains 2 ADF-H domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 341›341Twinfilin-2
PRO_0000233138

Regions

Domain‹1 – 131›131ADF-H 1
Domain169 – 305137ADF-H 2

Amino acid modifications

Modified residue61N6-acetyllysine By similarity
Modified residue3011Phosphotyrosine By similarity
Modified residue3411Phosphoserine By similarity

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
Q5RFH1 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: 98C5E459EB3413A5

FASTA34138,707
        10         20         30         40         50         60 
ATEELKEFFA KARAGSVRLI KVVIEDEQLV LGASQEPVGR WDRDYDRAVL PLLDAQQPCY 

        70         80         90        100        110        120 
LLYRLDSQNA QGFEWLFLAW SPDNSPVRLK MLYAATRATV KKEFGGGHIK DELFGTVKDD 

       130        140        150        160        170        180 
LSFAGYQKHL SSCAAPAPLT SAERELQQIR INEVKTEISV ESKHQTLQGL AFPLQPEAQR 

       190        200        210        220        230        240 
ALQQLKQKMV NYIQMKLDLE RETIELVHTE STDVAQLPSR VPRDAARYHF FLYKHTHEGD 

       250        260        270        280        290        300 
LLESVVFIYS MPGYKCSIEE RMLYSSCKSR LLDSVEQDFH LEIAKKIEIG DGAELTAEFL 

       310        320        330        340 
YDEVHPKQHA FKQAFAKPKG PGGKRGHKRL IRGPGENGDD S 

« Hide

References

[1]The German cDNA consortium
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Heart.

Web resources

Protein Spotlight

Molecular embrace - Issue 73 of August 2006

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR857187 Transcribed RNA. Translation: CAH89486.1.

3D structure databases

ProteinModelPortalQ5RFH1.
SMRQ5RFH1. Positions 1-129, 153-305.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG000848.
InParanoidQ5RFH1.

Family and domain databases

InterProIPR002108. ADF-H.
IPR028458. Twinfilin.
[Graphical view]
PANTHERPTHR13759. PTHR13759. 1 hit.
PfamPF00241. Cofilin_ADF. 2 hits.
[Graphical view]
SMARTSM00102. ADF. 2 hits.
[Graphical view]
PROSITEPS51263. ADF_H. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTWF2_PONAB
AccessionPrimary (citable) accession number: Q5RFH1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: December 21, 2004
Last modified: April 16, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries