Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q5RFH1

- TWF2_PONAB

UniProt

Q5RFH1 - TWF2_PONAB

Protein

Twinfilin-2

Gene

TWF2

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (21 Dec 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. May play a role in regulating the mature length of the middle and short rows of stereocilia By similarity.By similarity

    GO - Biological processi

    1. cell projection organization Source: UniProtKB-KW
    2. negative regulation of actin filament polymerization Source: InterPro

    Keywords - Biological processi

    Cilium biogenesis/degradation

    Keywords - Ligandi

    Actin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Twinfilin-2
    Gene namesi
    Name:TWF2
    OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
    Taxonomic identifieri9601 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
    ProteomesiUP000001595: Unplaced

    Subcellular locationi

    Cytoplasmcytoskeleton By similarity. Cytoplasmperinuclear region By similarity. Cell projectionstereocilium By similarity
    Note: Perinuclear and G-actin-rich cortical actin structure sublocalization.By similarity

    GO - Cellular componenti

    1. cytoskeleton Source: UniProtKB-SubCell
    2. perinuclear region of cytoplasm Source: UniProtKB-SubCell
    3. stereocilium Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell projection, Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – 341›341Twinfilin-2PRO_0000233138Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei6 – 61N6-acetyllysineBy similarity
    Modified residuei301 – 3011PhosphotyrosineBy similarity
    Modified residuei341 – 3411PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated on both serine and threonine residues.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Interactioni

    Subunit structurei

    Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF1 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down-regulated. Interacts with MYO7A By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ5RFH1.
    SMRiQ5RFH1. Positions 1-129, 153-305.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini‹1 – 131›131ADF-H 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini169 – 305137ADF-H 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 2 ADF-H domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    HOVERGENiHBG000848.
    InParanoidiQ5RFH1.

    Family and domain databases

    Gene3Di3.40.20.10. 2 hits.
    InterProiIPR002108. ADF-H.
    IPR029006. ADF-H/Gelsolin-like_dom.
    IPR028458. Twinfilin.
    [Graphical view]
    PANTHERiPTHR13759. PTHR13759. 1 hit.
    PfamiPF00241. Cofilin_ADF. 2 hits.
    [Graphical view]
    SMARTiSM00102. ADF. 2 hits.
    [Graphical view]
    PROSITEiPS51263. ADF_H. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Q5RFH1-1 [UniParc]FASTAAdd to Basket

    « Hide

    ATEELKEFFA KARAGSVRLI KVVIEDEQLV LGASQEPVGR WDRDYDRAVL    50
    PLLDAQQPCY LLYRLDSQNA QGFEWLFLAW SPDNSPVRLK MLYAATRATV 100
    KKEFGGGHIK DELFGTVKDD LSFAGYQKHL SSCAAPAPLT SAERELQQIR 150
    INEVKTEISV ESKHQTLQGL AFPLQPEAQR ALQQLKQKMV NYIQMKLDLE 200
    RETIELVHTE STDVAQLPSR VPRDAARYHF FLYKHTHEGD LLESVVFIYS 250
    MPGYKCSIEE RMLYSSCKSR LLDSVEQDFH LEIAKKIEIG DGAELTAEFL 300
    YDEVHPKQHA FKQAFAKPKG PGGKRGHKRL IRGPGENGDD S 341
    Length:341
    Mass (Da):38,707
    Last modified:December 21, 2004 - v1
    Checksum:i98C5E459EB3413A5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR857187 Transcribed RNA. Translation: CAH89486.1.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    Molecular embrace - Issue 73 of August 2006

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR857187 Transcribed RNA. Translation: CAH89486.1 .

    3D structure databases

    ProteinModelPortali Q5RFH1.
    SMRi Q5RFH1. Positions 1-129, 153-305.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG000848.
    InParanoidi Q5RFH1.

    Family and domain databases

    Gene3Di 3.40.20.10. 2 hits.
    InterProi IPR002108. ADF-H.
    IPR029006. ADF-H/Gelsolin-like_dom.
    IPR028458. Twinfilin.
    [Graphical view ]
    PANTHERi PTHR13759. PTHR13759. 1 hit.
    Pfami PF00241. Cofilin_ADF. 2 hits.
    [Graphical view ]
    SMARTi SM00102. ADF. 2 hits.
    [Graphical view ]
    PROSITEi PS51263. ADF_H. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. The German cDNA consortium
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Heart.

    Entry informationi

    Entry nameiTWF2_PONAB
    AccessioniPrimary (citable) accession number: Q5RFH1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 2, 2006
    Last sequence update: December 21, 2004
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3