Reviewed, UniProtKB/Swiss-Prot Q5RFG2 (BHMT1_PONAB)

Names and origin

Protein names

Recommended name:
    Betaine--homocysteine S-methyltransferase 1
    EC=2.1.1.5

Gene names

Name:

BHMT

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline By similarity.
Catalytic activity	Trimethylammonioacetate + L-homocysteine = dimethylglycine + L-methionine.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 Hcy-binding domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	betaine-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	Betaine--homocysteine S-methyltransferase 1		PRO_0000234128
Regions
	Domain	11 – 314	304	Hcy-binding
Sites
	Metal binding	217	1	Zinc Potential
	Metal binding	299	1	Zinc Potential
	Metal binding	300	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5RFG2-1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 6BCCB007E7ED5B8B	FASTA	406	45,112
	10 20 30 40 50 60 MPPVVGKKAK KGILERLNAG EIVIGDGGFV FALEKRGYVK AGPWTPEAAV EHPEAVRQLH 70 80 90 100 110 120 REFLRAGSNV MQTFTFYASE DKLENRGNYV LEKISGQKVN EAACDIARQV ADEGDALVAG 130 140 150 160 170 180 GVSQTPSYLS CKSETEVKKV FLQQLEVFMK KNVDFLIAEY FEHVEEAVWA VETLIASGKP 190 200 210 220 230 240 VAATMCIGPE GDLHGVPPGE CAVRLVKAGA SIIGVNCHFD PTISLKTVKL MKEGLEAARL 250 260 270 280 290 300 KAHLMSQPLA YHTPDCNKQG FIDLPEFPFG LEPRVATRWD IQKYAREAYN MGIRYIGGCC 310 320 330 340 350 360 GFEPYHIRAI AEELAPERGF LPPASEKHGS WGSALDMHTK PWVRARARKE YWENLRIASG 370 380 390 400 RPYNPSMSKP DGWGVTKGTA ELMQQKEATT EQQLKELFEK QKFKLQ

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR857196 mRNA. Translation: CAH89495.1.
RefSeq	NP_001127156.1.
UniGene	Pab.19523
3D structure databases
SMR	Q5RFG2. Positions 11-371.
ModBase	Search...
Genome annotation databases
GeneID	100174207.
Organism-specific databases
CTD	100174207.
Phylogenomic databases
HOVERGEN	Q5RFG2.
Enzyme and pathway databases
BRENDA	2.1.1.5. 269192.
Family and domain databases
InterPro	IPR017226. Betaine-hCys_S-MeTrfase_BHMT. IPR003726. S_MeTrfase. [Graphical view]
Gene3D	G3DSA:3.20.20.330. S_methyl_trans. 1 hit.
Pfam	PF02574. S-methyl_trans. 1 hit. [Graphical view]
PIRSF	PIRSF037505. Betaine_HMT. 1 hit.
PROSITE	PS50970. HCY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BHMT1_PONAB

Accession

Primary (citable) accession number: Q5RFG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	December 21, 2004
Last modified:	September 1, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)