Reviewed, UniProtKB/Swiss-Prot Q5RFB0 (ACY1_PONAB)

Names and origin

Protein names

Recommended name:
    Aminoacylase-1
    EC=3.5.1.14
Alternative name(s):
    N-acyl-L-amino-acid amidohydrolase
    ACY-1

Gene names

Name:

ACY1

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	408 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate) By similarity.
Catalytic activity	An N-acyl-L-amino acid + H2O = a carboxylate + an L-amino acid.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subunit structure	Homodimer By similarity. Interacts with SPHK1 By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M20A family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminoacylase activity Inferred from electronic annotation. Source: EC metallopeptidase activity Inferred from electronic annotation. Source: InterPro protein dimerization activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 408	408	Aminoacylase-1		PRO_0000274008
Sites
	Active site	82	1	By similarity
	Active site	147	1	Proton acceptor By similarity
	Metal binding	80	1	Zinc 1 By similarity
	Metal binding	113	1	Zinc 1 By similarity
	Metal binding	113	1	Zinc 2 By similarity
	Metal binding	148	1	Zinc 2 By similarity
	Metal binding	175	1	Zinc 1 By similarity
	Metal binding	373	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5RFB0-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: DA5F3D6468261C84	FASTA	408	45,969
	10 20 30 40 50 60 MTSKGPEEEH PSVTLFRQYL RIRTVQPKPD YGAAVAFFEE RARQLGLGCQ KVEVAPGYVV 70 80 90 100 110 120 TVLTWPGTNP TLSSILLNSH TDVVPVFKEH WSHDPFEAFK DSEGYIYARG AQDMKCISIQ 130 140 150 160 170 180 YLEAVRRLKV EGHRFPRTIH MTFVPDEEVG GHQGMELFVQ RPEFHALRAG FALDEGIANP 190 200 210 220 230 240 TDAFTVFYSE RSPWWVRVTS TGRPGHASRF MEDTAAEKLH KVVSSILAFR EKEWQRLQSN 250 260 270 280 290 300 PHLKEGSVTS VNLTKLEGGV AYNVIPATMS ASFDFRVAPD VDFKAFEEQL QSWCQAAGEG 310 320 330 340 350 360 VTLEFAQKWM HPQVTPTDDS NPWWAAFSRV CKDMKLTLEP EIMPAATDNR YIRAVGIPAL 370 380 390 400 GFSPMNRTPV LLHDHDERLH EAVFLRGVDI YTRLLPALAS VPALPSES

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR857251 mRNA. Translation: CAH89547.1. Different initiation.
RefSeq	NP_001124673.1.
UniGene	Pab.19502
3D structure databases
SMR	Q5RFB0. Positions 7-198, 321-408.
ModBase	Search...
Genome annotation databases
GeneID	100171519.
Organism-specific databases
CTD	100171519.
Phylogenomic databases
HOVERGEN	Q5RFB0.
Enzyme and pathway databases
BRENDA	3.5.1.14. 269192.
Family and domain databases
InterPro	IPR001261. ArgE/DapE_CS. IPR010159. N-acyl_aa_amidohydrolase. IPR002933. Peptidase_M20. IPR011650. Peptidase_M20_dimer. [Graphical view]
Pfam	PF07687. M20_dimer. 1 hit. PF01546. Peptidase_M20. 1 hit. [Graphical view]
PIRSF	PIRSF036696. ACY-1. 1 hit.
TIGRFAMs	TIGR01880. Ac-peptdase-euk. 1 hit.
PROSITE	PS00758. ARGE_DAPE_CPG2_1. 1 hit. PS00759. ARGE_DAPE_CPG2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACY1_PONAB

Accession

Primary (citable) accession number: Q5RFB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	January 23, 2007
Last modified:	September 22, 2009
This is version 30 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)