Reviewed, UniProtKB/Swiss-Prot Q5RFA6 (ALDOB_PONAB)

Names and origin

Protein names

Recommended name:
    Fructose-bisphosphate aldolase B
    EC=4.1.2.13
Alternative name(s):
    Liver-type aldolase

Gene names

Name:

ALDOB

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Subunit structure	Homotetramer By similarity.
Miscellaneous	In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain.
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Schiff base
Molecular function	Lyase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 364	363	Fructose-bisphosphate aldolase B		PRO_0000230296
Sites
	Active site	188	1	Proton acceptor By similarity
	Active site	230	1	Schiff-base intermediate with dihydroxyacetone-P By similarity
	Binding site	56	1	Substrate By similarity
	Binding site	147	1	Substrate By similarity
	Site	364	1	Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	36	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5RFA6-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 992D2580A7F34CB9	FASTA	364	39,447
	10 20 30 40 50 60 MAHRFPALTQ EQKKELSEIA QSIVANGKGI LAADESVGTM GNRLQRIKVE NTEENRRQFR 70 80 90 100 110 120 EILFSVDSSI NQSIGGVILF HETLYQKDSQ GKLFRNILKE KGIVVGIKLD QGGAPLAGTN 130 140 150 160 170 180 KETTIQGLDG LSERCAQYKK DGVDFGKWRA VLRIADQCPS SLAIQENANA LARYASICQQ 190 200 210 220 230 240 NGLVPIVEPE VIPDGDHDLE HCQYVTEKVL AAAYKALNDH HVYLEGTLLK PNMVTAGHAC 250 260 270 280 290 300 TKKYTPEQVA MATVTALHRT VPAAVPGICF LSGGMSEEDA TLNLNAINLC PLPKPWKLSF 310 320 330 340 350 360 SYGRALQASA LAAWGGKAAN KEATQEAFMK RAVANHQAAK GQYVHTGSSG AASTQSLFTA CYTY

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR857255 mRNA. Translation: CAH89551.1.
RefSeq	NP_001127166.1.
UniGene	Pab.18115
3D structure databases
SMR	Q5RFA6. Positions 1-363, 2-364.
ModBase	Search...
Genome annotation databases
GeneID	100174217.
Organism-specific databases
CTD	100174217.
Phylogenomic databases
HOVERGEN	Q5RFA6.
Enzyme and pathway databases
BRENDA	4.1.2.13. 269192.
Family and domain databases
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11627. Aldolase_I. 1 hit.
Pfam	PF00274. Glycolytic. 1 hit. [Graphical view]
ProDom	PD001128. Aldolase_I. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00158. ALDOLASE_CLASS_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALDOB_PONAB

Accession

Primary (citable) accession number: Q5RFA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	January 23, 2007
Last modified:	September 1, 2009
This is version 33 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)