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Protein

Cytosolic beta-glucosidase

Gene

GBA3

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Seems to be involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. Able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. Possesses beta-glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide (By similarity).By similarity

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei17 – 171SubstrateBy similarity
Binding sitei120 – 1201SubstrateBy similarity
Binding sitei164 – 1641SubstrateBy similarity
Active sitei165 – 1651Proton donorSequence Analysis
Binding sitei309 – 3091SubstrateBy similarity
Active sitei373 – 3731NucleophileSequence Analysis
Binding sitei417 – 4171SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic beta-glucosidase (EC:3.2.1.21)
Gene namesi
Name:GBA3
Synonyms:CBG
OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Taxonomic identifieri9601 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
ProteomesiUP000001595 Componenti: Chromosome 4

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469Cytosolic beta-glucosidasePRO_0000063909Add
BLAST

Post-translational modificationi

The N-terminus is blocked.By similarity

Interactioni

Protein-protein interaction databases

STRINGi9601.ENSPPYP00000016355.

Structurei

3D structure databases

ProteinModelPortaliQ5RF65.
SMRiQ5RF65. Positions 1-466.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni424 – 4252Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000074452.
HOVERGENiHBG053101.
InParanoidiQ5RF65.
KOiK05350.
OMAiISHYEIP.
OrthoDBiEOG7MH0XV.
TreeFamiTF314803.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5RF65-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFPVGFGWA AATAAYQVEG GWDADGKGPC VWDTFTHQGG ERVFKNQTGD
60 70 80 90 100
VACGSYTLWE EDLKCIKQLG LTHYRFSLSW SRLLPDGTTG FINQKGIDYY
110 120 130 140 150
NKIIDDLLKN GVTPIVTLYH FDLPQALEDQ GGWLSEAIIE SFDKYAQFCF
160 170 180 190 200
STFGDRVKKW ITINEANVLS VMSYDLGMFP PGIPHFGTGG YQAAHNLIKA
210 220 230 240 250
HARSWHSYNS LFRKEQKGMV SLSLFAVWLE PADPNSVSDQ EAAKRAITFH
260 270 280 290 300
LDLFAKPIFI DGDYPEIVKS QIASMSQKQG YPSSRLPEFT EEEKKMIKGT
310 320 330 340 350
ADFFAVQYYT TRLIKYQENK KGELGILQDA EIEFFPDPSW KNVDWIYVVP
360 370 380 390 400
WGVRKLLKYI KDTYNNPVIY ITENGFPQSD PAPLDDTQRW EYFRQTFQEL
410 420 430 440 450
FKAIQLDKVN LQVYCAWSLL DNFEWNQGYS SRFGLFHVDF EDPARPRVPY
460
TSAKEYAKVI RNNGLEAHL
Length:469
Mass (Da):53,747
Last modified:December 21, 2004 - v1
Checksum:iEC6DFA9543EA6843
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR857296 mRNA. Translation: CAH89592.1.
RefSeqiNP_001124705.1. NM_001131233.1.

Genome annotation databases

EnsembliENSPPYT00000017020; ENSPPYP00000016355; ENSPPYG00000014639.
GeneIDi100171553.
KEGGipon:100171553.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR857296 mRNA. Translation: CAH89592.1.
RefSeqiNP_001124705.1. NM_001131233.1.

3D structure databases

ProteinModelPortaliQ5RF65.
SMRiQ5RF65. Positions 1-466.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9601.ENSPPYP00000016355.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSPPYT00000017020; ENSPPYP00000016355; ENSPPYG00000014639.
GeneIDi100171553.
KEGGipon:100171553.

Organism-specific databases

CTDi57733.

Phylogenomic databases

GeneTreeiENSGT00550000074452.
HOVERGENiHBG053101.
InParanoidiQ5RF65.
KOiK05350.
OMAiISHYEIP.
OrthoDBiEOG7MH0XV.
TreeFamiTF314803.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. The German cDNA consortium
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiGBA3_PONAB
AccessioniPrimary (citable) accession number: Q5RF65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: December 21, 2004
Last modified: July 22, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.