Reviewed,
UniProtKB/Swiss-Prot Q5RF65 (GBA3_PONAB)
Last modified
February 9, 2010.
Version 36.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Cytosolic beta-glucosidase EC=3.2.1.21 | ||||
| Gene names |
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| Organism | Pongo abelii (Sumatran orangutan) | ||||
| Taxonomic identifier | 9601 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo |
Protein attributes
| Sequence length | 469 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | Seems to be involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. Able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. Possesses beta-glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide By similarity. |
| Catalytic activity | Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose. |
| Subcellular location | |
| Post-translational modification | The N-terminus is blocked By similarity. |
| Sequence similarities | Belongs to the glycosyl hydrolase 1 family. Klotho subfamily. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm |
| Molecular function | Glycosidase Hydrolase |
| Gene Ontology (GO) | |
| Biological process | carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro glycosylceramide catabolic processInferred from sequence or structural similarity. Source: UniProtKB |
| Cellular component | cytosol Inferred from sequence or structural similarity. Source: UniProtKB |
| Molecular function | beta-galactosidase activity Inferred from sequence or structural similarity. Source: UniProtKB beta-glucosidase activityInferred from sequence or structural similarity. Source: UniProtKB cation bindingInferred from electronic annotation. Source: InterPro glycosylceramidase activityInferred from sequence or structural similarity. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 469 | 469 | Cytosolic beta-glucosidase | PRO_0000063909 | |||||
Regions | |||||||||
| Region | 424 – 425 | 2 | Substrate binding By similarity | ||||||
Sites | |||||||||
| Active site | 165 | 1 | Proton donor Potential | ||||||
| Active site | 373 | 1 | Nucleophile Potential | ||||||
| Binding site | 17 | 1 | Substrate By similarity | ||||||
| Binding site | 120 | 1 | Substrate By similarity | ||||||
| Binding site | 164 | 1 | Substrate By similarity | ||||||
| Binding site | 309 | 1 | Substrate By similarity | ||||||
| Binding site | 417 | 1 | Substrate By similarity | ||||||
Sequences
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References
| [1] | The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | CR857296 mRNA. Translation: CAH89592.1. |
| RefSeq | NP_001124705.1. |
| UniGene | Pab.19483 |
3D structure databases | |
| SMR | Q5RF65. Positions 1-466. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | GH1. Glycoside Hydrolase Family 1. |
Genome annotation databases | |
| GeneID | 100171553. |
Organism-specific databases | |
| CTD | 100171553. |
Phylogenomic databases | |
| HOVERGEN | Q5RF65. |
| InParanoid | Q5RF65. |
Enzyme and pathway databases | |
| BRENDA | 3.2.1.21. 269192. |
Family and domain databases | |
| InterPro | IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view] |
| Gene3D | G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. |
| PANTHER | PTHR10353. Glyco_hydro_1. 1 hit. |
| Pfam | PF00232. Glyco_hydro_1. 1 hit. [Graphical view] |
| PRINTS | PR00131. GLHYDRLASE1. |
| PROSITE | PS00572. GLYCOSYL_HYDROL_F1_1. False negative. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | GBA3_PONAB | ||||||||
| Accession | Primary (citable) accession number: Q5RF65 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


