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Q5RF65

- GBA3_PONAB

UniProt

Q5RF65 - GBA3_PONAB

Protein

Cytosolic beta-glucosidase

Gene

GBA3

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (21 Dec 2004)
      Previous versions | rss
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    Functioni

    Seems to be involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. Able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. Possesses beta-glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide By similarity.By similarity

    Catalytic activityi

    Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei17 – 171SubstrateBy similarity
    Binding sitei120 – 1201SubstrateBy similarity
    Binding sitei164 – 1641SubstrateBy similarity
    Active sitei165 – 1651Proton donorSequence Analysis
    Binding sitei309 – 3091SubstrateBy similarity
    Active sitei373 – 3731NucleophileSequence Analysis
    Binding sitei417 – 4171SubstrateBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB
    2. beta-glucosidase activity Source: UniProtKB
    3. glycosylceramidase activity Source: UniProtKB

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. glycosylceramide catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytosolic beta-glucosidase (EC:3.2.1.21)
    Gene namesi
    Name:GBA3
    Synonyms:CBG
    OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
    Taxonomic identifieri9601 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
    ProteomesiUP000001595: Chromosome 4

    Subcellular locationi

    Cytoplasmcytosol By similarity

    GO - Cellular componenti

    1. cytosol Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 469469Cytosolic beta-glucosidasePRO_0000063909Add
    BLAST

    Post-translational modificationi

    The N-terminus is blocked.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ5RF65.
    SMRiQ5RF65. Positions 1-466.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni424 – 4252Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    GeneTreeiENSGT00550000074452.
    HOVERGENiHBG053101.
    InParanoidiQ5RF65.
    KOiK05350.
    OMAiALMSKKQ.
    OrthoDBiEOG7MH0XV.
    TreeFamiTF314803.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5RF65-1 [UniParc]FASTAAdd to Basket

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    MAFPVGFGWA AATAAYQVEG GWDADGKGPC VWDTFTHQGG ERVFKNQTGD    50
    VACGSYTLWE EDLKCIKQLG LTHYRFSLSW SRLLPDGTTG FINQKGIDYY 100
    NKIIDDLLKN GVTPIVTLYH FDLPQALEDQ GGWLSEAIIE SFDKYAQFCF 150
    STFGDRVKKW ITINEANVLS VMSYDLGMFP PGIPHFGTGG YQAAHNLIKA 200
    HARSWHSYNS LFRKEQKGMV SLSLFAVWLE PADPNSVSDQ EAAKRAITFH 250
    LDLFAKPIFI DGDYPEIVKS QIASMSQKQG YPSSRLPEFT EEEKKMIKGT 300
    ADFFAVQYYT TRLIKYQENK KGELGILQDA EIEFFPDPSW KNVDWIYVVP 350
    WGVRKLLKYI KDTYNNPVIY ITENGFPQSD PAPLDDTQRW EYFRQTFQEL 400
    FKAIQLDKVN LQVYCAWSLL DNFEWNQGYS SRFGLFHVDF EDPARPRVPY 450
    TSAKEYAKVI RNNGLEAHL 469
    Length:469
    Mass (Da):53,747
    Last modified:December 21, 2004 - v1
    Checksum:iEC6DFA9543EA6843
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR857296 mRNA. Translation: CAH89592.1.
    RefSeqiNP_001124705.1. NM_001131233.1.

    Genome annotation databases

    EnsembliENSPPYT00000017020; ENSPPYP00000016355; ENSPPYG00000014639.
    GeneIDi100171553.
    KEGGipon:100171553.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR857296 mRNA. Translation: CAH89592.1 .
    RefSeqi NP_001124705.1. NM_001131233.1.

    3D structure databases

    ProteinModelPortali Q5RF65.
    SMRi Q5RF65. Positions 1-466.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSPPYT00000017020 ; ENSPPYP00000016355 ; ENSPPYG00000014639 .
    GeneIDi 100171553.
    KEGGi pon:100171553.

    Organism-specific databases

    CTDi 57733.

    Phylogenomic databases

    GeneTreei ENSGT00550000074452.
    HOVERGENi HBG053101.
    InParanoidi Q5RF65.
    KOi K05350.
    OMAi ALMSKKQ.
    OrthoDBi EOG7MH0XV.
    TreeFami TF314803.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. The German cDNA consortium
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.

    Entry informationi

    Entry nameiGBA3_PONAB
    AccessioniPrimary (citable) accession number: Q5RF65
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2005
    Last sequence update: December 21, 2004
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3