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Q5RF65 (GBA3_PONAB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytosolic beta-glucosidase

EC=3.2.1.21
Gene names
Name:GBA3
Synonyms:CBG
OrganismPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) [Reference proteome]
Taxonomic identifier9601 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo

Protein attributes

Sequence length469 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Seems to be involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. Able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. Possesses beta-glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide By similarity.

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Subcellular location

Cytoplasmcytosol By similarity.

Post-translational modification

The N-terminus is blocked By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 469469Cytosolic beta-glucosidase
PRO_0000063909

Regions

Region424 – 4252Substrate binding By similarity

Sites

Active site1651Proton donor Potential
Active site3731Nucleophile Potential
Binding site171Substrate By similarity
Binding site1201Substrate By similarity
Binding site1641Substrate By similarity
Binding site3091Substrate By similarity
Binding site4171Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5RF65 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: EC6DFA9543EA6843

FASTA46953,747
        10         20         30         40         50         60 
MAFPVGFGWA AATAAYQVEG GWDADGKGPC VWDTFTHQGG ERVFKNQTGD VACGSYTLWE 

        70         80         90        100        110        120 
EDLKCIKQLG LTHYRFSLSW SRLLPDGTTG FINQKGIDYY NKIIDDLLKN GVTPIVTLYH 

       130        140        150        160        170        180 
FDLPQALEDQ GGWLSEAIIE SFDKYAQFCF STFGDRVKKW ITINEANVLS VMSYDLGMFP 

       190        200        210        220        230        240 
PGIPHFGTGG YQAAHNLIKA HARSWHSYNS LFRKEQKGMV SLSLFAVWLE PADPNSVSDQ 

       250        260        270        280        290        300 
EAAKRAITFH LDLFAKPIFI DGDYPEIVKS QIASMSQKQG YPSSRLPEFT EEEKKMIKGT 

       310        320        330        340        350        360 
ADFFAVQYYT TRLIKYQENK KGELGILQDA EIEFFPDPSW KNVDWIYVVP WGVRKLLKYI 

       370        380        390        400        410        420 
KDTYNNPVIY ITENGFPQSD PAPLDDTQRW EYFRQTFQEL FKAIQLDKVN LQVYCAWSLL 

       430        440        450        460 
DNFEWNQGYS SRFGLFHVDF EDPARPRVPY TSAKEYAKVI RNNGLEAHL 

« Hide

References

[1]The German cDNA consortium
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR857296 mRNA. Translation: CAH89592.1.
RefSeqNP_001124705.1. NM_001131233.1.

3D structure databases

ProteinModelPortalQ5RF65.
SMRQ5RF65. Positions 1-466.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSPPYT00000017020; ENSPPYP00000016355; ENSPPYG00000014639.
GeneID100171553.
KEGGpon:100171553.

Organism-specific databases

CTD57733.

Phylogenomic databases

GeneTreeENSGT00550000074452.
HOVERGENHBG053101.
InParanoidQ5RF65.
KOK05350.
OMAWNQGYSS.
OrthoDBEOG7MH0XV.
TreeFamTF314803.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGBA3_PONAB
AccessionPrimary (citable) accession number: Q5RF65
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: December 21, 2004
Last modified: February 19, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries