Reviewed, UniProtKB/Swiss-Prot Q5RF65 (GBA3_PONAB)

Names and origin

Protein names

Recommended name:
    Cytosolic beta-glucosidase
    EC=3.2.1.21

Gene names

Name:	GBA3
Synonyms:	CBG

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Seems to be involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. Able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. Possesses beta-glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide By similarity.
Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.
Subcellular location	Cytoplasm › cytosol By similarity.
Post-translational modification	The N-terminus is blocked By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro glycosylceramide catabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	beta-galactosidase activity Inferred from sequence or structural similarity. Source: UniProtKB beta-glucosidase activity Inferred from sequence or structural similarity. Source: UniProtKB cation binding Inferred from electronic annotation. Source: InterPro glycosylceramidase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Cytosolic beta-glucosidase		PRO_0000063909
Regions
	Region	424 – 425	2	Substrate binding By similarity
Sites
	Active site	165	1	Proton donor Potential
	Active site	373	1	Nucleophile Potential
	Binding site	17	1	Substrate By similarity
	Binding site	120	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	309	1	Substrate By similarity
	Binding site	417	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5RF65-1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: EC6DFA9543EA6843	FASTA	469	53,747
	10 20 30 40 50 60 MAFPVGFGWA AATAAYQVEG GWDADGKGPC VWDTFTHQGG ERVFKNQTGD VACGSYTLWE 70 80 90 100 110 120 EDLKCIKQLG LTHYRFSLSW SRLLPDGTTG FINQKGIDYY NKIIDDLLKN GVTPIVTLYH 130 140 150 160 170 180 FDLPQALEDQ GGWLSEAIIE SFDKYAQFCF STFGDRVKKW ITINEANVLS VMSYDLGMFP 190 200 210 220 230 240 PGIPHFGTGG YQAAHNLIKA HARSWHSYNS LFRKEQKGMV SLSLFAVWLE PADPNSVSDQ 250 260 270 280 290 300 EAAKRAITFH LDLFAKPIFI DGDYPEIVKS QIASMSQKQG YPSSRLPEFT EEEKKMIKGT 310 320 330 340 350 360 ADFFAVQYYT TRLIKYQENK KGELGILQDA EIEFFPDPSW KNVDWIYVVP WGVRKLLKYI 370 380 390 400 410 420 KDTYNNPVIY ITENGFPQSD PAPLDDTQRW EYFRQTFQEL FKAIQLDKVN LQVYCAWSLL 430 440 450 460 DNFEWNQGYS SRFGLFHVDF EDPARPRVPY TSAKEYAKVI RNNGLEAHL

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR857296 mRNA. Translation: CAH89592.1.
RefSeq	NP_001124705.1.
UniGene	Pab.19483
3D structure databases
SMR	Q5RF65. Positions 1-466.
ModBase	Search...
Protein family/group databases
CAZy	GH1. Glycoside Hydrolase Family 1.
Genome annotation databases
GeneID	100171553.
Organism-specific databases
CTD	100171553.
Phylogenomic databases
HOVERGEN	Q5RF65.
InParanoid	Q5RF65.
Enzyme and pathway databases
BRENDA	3.2.1.21. 269192.
Family and domain databases
InterPro	IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. False negative. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GBA3_PONAB

Accession

Primary (citable) accession number: Q5RF65

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	December 21, 2004
Last modified:	February 9, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)