Reviewed, UniProtKB/Swiss-Prot Q5RF32 (BHMT2_PONAB)

Names and origin

Protein names

Recommended name:
    Betaine--homocysteine S-methyltransferase 2
    EC=2.1.1.5

Gene names

Name:

BHMT2

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline By similarity.
Catalytic activity	Trimethylammonioacetate + L-homocysteine = dimethylglycine + L-methionine.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Contains 1 Hcy-binding domain.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	betaine-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Betaine--homocysteine S-methyltransferase 2		PRO_0000273226
Regions
	Domain	11 – 305	295	Hcy-binding
Sites
	Metal binding	208	1	Zinc By similarity
	Metal binding	290	1	Zinc By similarity
	Metal binding	291	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5RF32-1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: D2B34B3F8EF4A103	FASTA	363	40,336
	10 20 30 40 50 60 MAPAGHPGAK RGILERLESG EVVIGDGSFL ITLEKRGYVK AGLWTPEAVI EHPDAVRQLH 70 80 90 100 110 120 MEFLRAGSNV MQTFTFSASE DNMESKWEDV NAAACDLARE VAGKGDALVA GGICQTSIYK 130 140 150 160 170 180 YHKDEARIKK LFRQQLEVFA WKNVDFLIAE YFEHVEEAVW AVEVLKESDR PVAVTMCISP 190 200 210 220 230 240 EGDMHDITPG ECAVRLVKAG ASIVGVNCRF GPETSLKTIE LMKEGLQRAG LKAHLMVQPL 250 260 270 280 290 300 GFHTPDCGKE GFVDLPEYPF GLESRAATRW DIQKYAREAY NQGVRYIGGC CGFEPYHIRA 310 320 330 340 350 360 IAEELAPERG FLPPASEKHG SWGSALDMHT KPWVRARARR EYWENLLPAS GRPFCPSLSK PDV

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR857329 mRNA. Translation: CAH89625.1.
RefSeq	NP_001127177.1.
UniGene	Pab.19464
3D structure databases
SMR	Q5RF32. Positions 10-362.
ModBase	Search...
Genome annotation databases
GeneID	100174229.
Organism-specific databases
CTD	100174229.
Phylogenomic databases
HOVERGEN	Q5RF32.
OMA	KGGFVDL.
Enzyme and pathway databases
BRENDA	2.1.1.5. 269192.
Family and domain databases
InterPro	IPR017226. Betaine-hCys_S-MeTrfase_BHMT. IPR003726. S_MeTrfase. [Graphical view]
Gene3D	G3DSA:3.20.20.330. S_methyl_trans. 1 hit.
Pfam	PF02574. S-methyl_trans. 1 hit. [Graphical view]
PIRSF	PIRSF037505. Betaine_HMT. 1 hit.
PROSITE	PS50970. HCY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BHMT2_PONAB

Accession

Primary (citable) accession number: Q5RF32

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	December 21, 2004
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)