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Protein

N-alpha-acetyltransferase 50

Gene

NAA50

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

N-alpha-acetyltransferase that acetylates the N-terminus of proteins that retain their initiating methionine. Has a broad substrate specificity: able to acetylate the initiator methionine of most peptides, except for those with a proline in second position. Also displays N-epsilon-acetyltransferase activity by mediating acetylation of the side chain of specific lysines on proteins. Autoacetylates in vivo. The relevance of N-epsilon-acetyltransferase activity is however unclear: able to acetylate H4 in vitro, but this result has not been confirmed in vivo. Component of a N-alpha-acetyltransferase complex containing NAA10 and NAA15, but NAA50 does not influence the acetyltransferase activity of NAA10: this multiprotein complex probably constitutes the major contributor for N-terminal acetylation at the ribosome exit tunnel, with NAA10 acetylating all amino termini that are devoid of methionine and NAA50 acetylating other peptides. Required for sister chromatid cohesion during mitosis by promoting binding of CDCA5/sororin to cohesin: may act by counteracting the function of NAA10.By similarity

Catalytic activityi

Acetyl-CoA + an N-terminal-L-methionyl-L-alanyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-alanyl-[protein] + CoA.By similarity
Acetyl-CoA + an N-terminal-L-methionyl-L-seryl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-seryl-[protein] + CoA.By similarity
Acetyl-CoA + an N-terminal-L-methionyl-L-valyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-valyl-[protein] + CoA.By similarity
Acetyl-CoA + an N-terminal-L-methionyl-L-threonyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-threonyl-[protein] + CoA.By similarity
Acetyl-CoA + an N-terminal-L-methionyl-L-lysyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-lysyl-[protein] + CoA.By similarity
Acetyl-CoA + an N-terminal-L-methionyl-L-leucyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-leucyl-[protein] + CoA.By similarity
Acetyl-CoA + an N-terminal-L-methionyl-L-phenylalanyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-phenylalanyl-[protein] + CoA.By similarity
Acetyl-CoA + an N-terminal-L-methionyl-L-tyrosyl-[protein] = an N-terminal-N(alpha)-acetyl-L-methionyl-L-tyrosyl-[protein] + CoA.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei31SubstrateBy similarity1
Active sitei73By similarity1
Binding sitei75SubstrateBy similarity1
Active sitei112By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
N-alpha-acetyltransferase 50 (EC:2.3.1.258By similarity)
Alternative name(s):
N-acetyltransferase NAT13
N-epsilon-acetyltransferase 50By similarity (EC:2.3.1.-By similarity)
NatE catalytic subunit
Gene namesi
Name:NAA50
Synonyms:NAT13
OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Taxonomic identifieri9601 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
Proteomesi
  • UP000001595 Componenti: Chromosome 3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002849041 – 169N-alpha-acetyltransferase 50Add BLAST169

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphothreonineBy similarity1
Modified residuei34N6-acetyllysineBy similarity1
Modified residuei37N6-acetyllysineBy similarity1
Modified residuei110PhosphotyrosineBy similarity1
Modified residuei140N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiQ5RF28.

Interactioni

Subunit structurei

Component of a complex composed of NAA50, NAA15 and NAA10 (By similarity). Interacts with NAA35 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9601.ENSPPYP00000015136.

Structurei

3D structure databases

ProteinModelPortaliQ5RF28.
SMRiQ5RF28.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 155N-acetyltransferasePROSITE-ProRule annotationAdd BLAST150

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni77 – 90Acetyl-CoA bindingBy similarityAdd BLAST14
Regioni117 – 126Coenzyme A bindingBy similarity10
Regioni138 – 141SubstrateBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3138. Eukaryota.
COG0456. LUCA.
GeneTreeiENSGT00390000009110.
HOVERGENiHBG060820.
InParanoidiQ5RF28.
KOiK20793.
OMAiIVETKEH.
OrthoDBiEOG091G0PAG.
TreeFamiTF314841.

Family and domain databases

InterProiView protein in InterPro
IPR016181. Acyl_CoA_acyltransferase.
IPR000182. GNAT_dom.
PfamiView protein in Pfam
PF00583. Acetyltransf_1. 1 hit.
SUPFAMiSSF55729. SSF55729. 1 hit.
PROSITEiView protein in PROSITE
PS51186. GNAT. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5RF28-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGSRIELGD VTPHNIKQLK RLNQVIFPVS YNDKFYKDVL EVGELAKLAY
60 70 80 90 100
FNDIAVGAVC CRVDHSQNQK RLYIMTLGCL APYRRLGIGT KMLNHVLNIC
110 120 130 140 150
EKDGTFDNIY LHVQISNESA IDFYRKFGFE IIETKKNYYK RIEPADAHVL
160
QKNLKVPSGQ NADVQKTDN
Length:169
Mass (Da):19,398
Last modified:December 21, 2004 - v1
Checksum:i153A8021B74655CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR857333 mRNA. Translation: CAH89629.1.
RefSeqiNP_001124730.1. NM_001131258.1.

Genome annotation databases

EnsembliENSPPYT00000015741; ENSPPYP00000015136; ENSPPYG00000013536.
GeneIDi100171579.
KEGGipon:100171579.

Similar proteinsi

Entry informationi

Entry nameiNAA50_PONAB
AccessioniPrimary (citable) accession number: Q5RF28
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: December 21, 2004
Last modified: September 27, 2017
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families