Q5RF14 (MIPEP_PONAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial intermediate peptidase
Short name=MIP
EC=3.4.24.59

Gene names

Name:

MIPEP

Organism

Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) [Reference proteome]

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Ponginae › Pongo

Protein attributes

Sequence length	713 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cleaves proteins, imported into the mitochondrion, to their mature size By similarity.
Catalytic activity	Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.
Cofactor	Binds 1 zinc ion By similarity.
Enzyme regulation	Activity is divalent cation-dependent. It is stimulated by manganese, magnesium or calcium ions and reversibly inhibited by zinc, cobalt and iron By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the peptidase M3 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Calcium Cobalt Iron Magnesium Manganese Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 35	35	Mitochondrion By similarity
	Chain	36 – 713	678	Mitochondrial intermediate peptidase		PRO_0000319049
Sites
	Active site	496	1	By similarity
	Metal binding	495	1	Zinc; catalytic By similarity
	Metal binding	499	1	Zinc; catalytic By similarity
	Metal binding	502	1	Zinc; catalytic By similarity
Amino acid modifications
	Modified residue	126	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5RF14 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 32FB7C32F5BD1FF9	FASTA	713	80,478
	10 20 30 40 50 60 MLCVGRLGGL GARAAALPPR RAGRGILEAG IRARRVSTSW SPVGAAFNVK PQGSRLDLFG 70 80 90 100 110 120 ERRGLFGVPE LSAPEGFHAA QEKALRKAEL LVGRACSTPP GPQTVLIFDE LSDSLCRVAD 130 140 150 160 170 180 LADFVKIAHP EPAFREAAEE ACRSIGTMVE KLNTNVDLYQ SLRKLLADKK LVDSLDPETR 190 200 210 220 230 240 RVAELFMFDF EISGIHLDKE KRKRAVDLNV KILDLSSTFL MGANFPNKIE KHLLPEHIRR 250 260 270 280 290 300 NFTSAGDHII IDGLHAESPD DLVREAAYKI FLYPNAGQLK CLEELLSSRD LLAKLVGYST 310 320 330 340 350 360 FSHRALQGTI AKNPETVMQF LEKLSDKLSE RTLKDFEMIR GMKMKLNPQN SEVMPWDPPY 370 380 390 400 410 420 YSGVIRAERY NIEPSLYCPF FSLGACMEGL NILLNRLLGI SLYAEQPAKG EVWSEDVRKL 430 440 450 460 470 480 AVVHESEGLL GYIYCDFFQR ADKPHQDCHF TIRGGRLKED GDYQLPVVVL MLNLPRSSRS 490 500 510 520 530 540 SPTLLTPGMM ENLFHEMGHA MHSMLGRTRY QHVTGTRCPT DFAEVPSILM EYFANDYRVV 550 560 570 580 590 600 NQFARHYQTG QPLPKNMVSR LCESKKVCAA ADMQLQVFYA TLDQIYHGKH PLRNSTTDIL 610 620 630 640 650 660 KETQEKFYGL PYVPDTAWQL RFSHLVGYGA KYYSYLMSRA VASMVWKECF LQDPFNRAAG 670 680 690 700 710 ERYRREMLAH GGGREPMLMV EGMLQKCPSV DDFVSALVSD LDLDFETFLM DSE

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR857347 mRNA. Translation: CAH89643.1.
RefSeq	NP_001124737.1. NM_001131265.1.
UniGene	Pab.19458.
3D structure databases
ProteinModelPortal	Q5RF14.
ModBase	Search...
Protein family/group databases
MEROPS	M03.006.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100171586.
KEGG	pon:100171586.
Organism-specific databases
CTD	4285.
Phylogenomic databases
HOGENOM	HOG000230535.
HOVERGEN	HBG008215.
InParanoid	Q5RF14.
KO	K01410.
Family and domain databases
Gene3D	1.10.1370.10. 2 hits. 1.20.1050.40. 1 hit. 3.40.390.10. 1 hit.
InterPro	IPR024079. MetalloPept_cat_dom. IPR024077. Neurolysin/TOP_dom2. IPR024080. Neurolysin/TOP_N. IPR001567. Pept_M3A_M3B. [Graphical view]
Pfam	PF01432. Peptidase_M3. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MIPEP_PONAB

Accession

Primary (citable) accession number: Q5RF14

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	December 21, 2004
Last modified:	March 6, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program