Reviewed, UniProtKB/Swiss-Prot Q5REY3 (PRDX3_PONAB)

Names and origin

Protein names

Recommended name:
    Thioredoxin-dependent peroxide reductase, mitochondrial
    EC=1.11.1.15
Alternative name(s):
    Peroxiredoxin-3

Gene names

Name:

PRDX3

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in redox regulation of the cell. Protects radical-sensitive enzymes from oxidative damage by a radical-generating system By similarity.
Catalytic activity	2 R'-SH + ROOH = R'-S-S-R' + H(2)O + ROH.
Subunit structure	Homodimer; disulfide-linked, upon oxidation By similarity. Binds MAP3K13 By similarity.
Subcellular location	MitochondrionBy similarity.
Miscellaneous	The active site is the redox-active Cys-108 oxidized to Cys-SOH. Cys-SOH rapidly reacts with Cys-229-SH of the other subunit to form an intermolecular disulfide with a concomitant homodimer formation. The enzyme may be subsequently regenerated by reduction of the disulfide by thioredoxin By similarity. Irreversibly inactivated by overoxidation of Cys-108 (to Cys-SO(3)H) upon oxidative stress By similarity.
Sequence similarities	Belongs to the ahpC/TSA family. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Redox-active center Transit peptide
Molecular function	Antioxidant Oxidoreductase Peroxidase
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	peroxiredoxin activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 62	62	Mitochondrion By similarity
	Chain	63 – 256	194	Thioredoxin-dependent peroxide reductase, mitochondrial By similarity		PRO_0000256858
Regions
	Domain	63 – 221	159	Thioredoxin
Sites
	Active site	108	1	Cysteine sulfenic acid (-SOH) intermediate By similarity
Amino acid modifications
	Disulfide bond	108		Interchain (with C-229); in linked form By similarity
	Disulfide bond	229		Interchain (with C-108); in linked form By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5REY3-1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: BF425B99C7794406	FASTA	256	27,700
	10 20 30 40 50 60 MAAAVGRLLR ASVARGVSAI PWGISATAAL RPAACGRTSL TNLLCSGSSQ AKLFSTSSSY 70 80 90 100 110 120 HAPAVTQHAP YFKGTAVVNG EFKDLSLDDF KGKYLVLFFY PLDFTFVCPT EIVAFSDKAN 130 140 150 160 170 180 EFHDVNCEVV AVSVDSHFSH LAWINTPRKN GGLGHMNIAL LSDLTKQISR DYGVLLEGSG 190 200 210 220 230 240 LALRGLFIID PNGVIKHLSV NDLPVGRSVE ETLRLVKAFQ YVETHGEVCP ANWTPDSPTI 250 KPNPAASKEY FQKVNQ

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR857380 mRNA. Translation: CAH89674.1.
RefSeq	NP_001127184.1.
3D structure databases
SMR	Q5REY3. Positions 63-223.
ModBase	Search...
Protein family/group databases
PeroxiBase	4500. Ppy2CysPrx03.
Genome annotation databases
GeneID	100174238.
Phylogenomic databases
HOVERGEN	Q5REY3.
Family and domain databases
InterPro	IPR000866. AhpC-TSA. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF00578. AhpC-TSA. 1 hit. [Graphical view]
PROSITE	PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PRDX3_PONAB

Accession

Primary (citable) accession number: Q5REY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	December 21, 2004
Last modified:	November 25, 2008
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)