Q5RES1 (MMP14_PONAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Matrix metalloproteinase-14
Short name=MMP-14
EC=3.4.24.80

Gene names

Name:

MMP14

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	582 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface By similarity. May be involved in actin cytoskeleton reorganization by cleaving PTK7 By similarity.
Catalytic activity	Endopeptidase activity. Activates progelatinase A by cleavage of the propeptide at 37-Asn-|-Leu-38. Other bonds hydrolyzed include 35-Gly-|-Ile-36 in the propeptide of collagenase 3, and 341-Asn-|-Phe-342, 441-Asp-|-Leu-442 and 354-Gln-|-Thr-355 in the aggrecan interglobular domain.
Cofactor	Binds 1 zinc ion per subunit By similarity. Calcium By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein Potential. Melanosome By similarity.
Domain	The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Sequence similarities	Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat Signal Transmembrane Transmembrane helix
Ligand	Calcium Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Cleavage on pair of basic residues Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular matrix Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Propeptide	21 – 111	91	Activation peptide		PRO_0000330604
	Chain	112 – 582	471	Matrix metalloproteinase-14		PRO_0000330605
Regions
	Topological domain	112 – 541	430	Extracellular Potential
	Transmembrane	542 – 562	21	Helical; Potential
	Topological domain	563 – 582	20	Cytoplasmic Potential
	Domain	323 – 366	44	Hemopexin-like 1
	Domain	368 – 412	45	Hemopexin-like 2
	Domain	415 – 461	47	Hemopexin-like 3
	Domain	463 – 508	46	Hemopexin-like 4
	Motif	91 – 98	8	Cysteine switch By similarity
Sites
	Active site	240	1	By similarity
	Metal binding	93	1	Zinc; in inhibited form By similarity
	Metal binding	239	1	Zinc; catalytic
	Metal binding	243	1	Zinc; catalytic
	Metal binding	249	1	Zinc; catalytic
Amino acid modifications
	Disulfide bond	319 ↔ 508		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5RES1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 9CC4480F6DEE9003	FASTA	582	65,832
	10 20 30 40 50 60 MSPAPRPSRC LLLPLLTLGT ALASLGSAQS SSFSPEAWLQ QYGYLPPGDL RTHTQRSPQS 70 80 90 100 110 120 LSAAIAAMQK FYGLRVTGKA DADTMKAMRR PRCGVPDKFG AEIKANVRRK RYAIQGLKWQ 130 140 150 160 170 180 HNEITFCIQN YTPKVGEYAT YEAIRKAFRV WESATPLRFR EVPYAYIREG HEKQADVMIF 190 200 210 220 230 240 FAEGFHGDST PFDGEGGFLA HAYFPGPNIG GDTHFDSAEP WTVGNEDLNG NDIFLVAVHE 250 260 270 280 290 300 LGHALGLEHS SDPSAIMAPF YQWMDTENFV LPEDDRRGIQ QLYGSESGFP TKMPPQPRTT 310 320 330 340 350 360 SRPSVPDKPK NPTYGPNTCD GNFDTVAMLR GEMFVFKERW FWRVRNNQVM DGYPMPIGQF 370 380 390 400 410 420 WRGLPASINT AYERKDGKFV FFKGDKHWVF DEASLEPGYP KHIKELGRGL PTDKIDAALF 430 440 450 460 470 480 WMPNGKTYFF RGNKYYRFNE ELRAVDSEYP KNIKVWEGIP ESPRGSFMGS DEVFTYFYKG 490 500 510 520 530 540 NKYWKFDNQK LKVEPGYPKS ALRDWMGCPS GGRPDEGTEE ETEVIIIEVD EEGGGAVSAA 550 560 570 580 AVVLPVLLLL LVLAVGLAVF FFRRHGTPRR LLYCQRSLLD KV

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR857445 mRNA. Translation: CAH89736.1.
RefSeq	NP_001124793.1. NM_001131321.1.
UniGene	Pab.12271.
3D structure databases
HSSP	HSSP built from PDB template 1BQQ based on UniProtKB P50281.
ProteinModelPortal	Q5RES1.
SMR	Q5RES1. Positions 114-287.
ModBase	Search...
Protein family/group databases
MEROPS	M10.014.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100171646.
KEGG	pon:100171646.
Organism-specific databases
CTD	4323.
Phylogenomic databases
HOVERGEN	HBG052484.
InParanoid	Q5RES1.
Family and domain databases
InterPro	IPR000585. Hemopexin/matrixin. IPR018486. Hemopexin/matrixin_CS. IPR018487. Hemopexin/matrixin_repeat. IPR024079. MetalloPept_cat_dom. IPR001818. Pept_M10_metallopeptidase. IPR016293. Pept_M10A_matrix_strom. IPR021190. Pept_M10A_matrixin. IPR021805. Pept_M10A_metallopeptidase_C. IPR021158. Pept_M10A_Zn_BS. IPR006026. Peptidase_Metallo. IPR002477. Peptidoglycan-bd-like. [Graphical view]
Gene3D	G3DSA:3.40.390.10. G3DSA:3.40.390.10. 1 hit. G3DSA:2.110.10.10. Hemopexin. 1 hit.
KO	K07763.
Pfam	PF11857. DUF3377. 1 hit. PF00045. Hemopexin. 4 hits. PF00413. Peptidase_M10. 1 hit. PF01471. PG_binding_1. 1 hit. [Graphical view]
PIRSF	PIRSF001191. Peptidase_M10A_matrix. 1 hit.
PRINTS	PR00138. MATRIXIN.
SMART	SM00120. HX. 4 hits. SM00235. ZnMc. 1 hit. [Graphical view]
SUPFAM	SSF50923. Hemopexin. 1 hit. SSF47090. PGBD_like. 1 hit.
PROSITE	PS00546. CYSTEINE_SWITCH. 1 hit. PS00024. HEMOPEXIN. 1 hit. PS00142. ZINC_PROTEASE. 1 hit. Uncertain. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MMP14_PONAB

Accession

Primary (citable) accession number: Q5RES1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	December 21, 2004
Last modified:	November 16, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program