Reviewed, UniProtKB/Swiss-Prot Q5REQ1 (HYAL3_PONAB)

Names and origin

Protein names

Recommended name:
    Hyaluronidase-3
      Short name=Hyal-3
    EC=3.2.1.35
Alternative name(s):
    Hyaluronoglucosaminidase-3

Gene names

Name:

HYAL3

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.
Subcellular location	Secreted By similarity. Lysosome By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 56 family. Contains 1 EGF-like domain.

Ontologies

Keywords
Cellular component	Lysosome Secreted
Domain	EGF-like domain Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hyalurononglucosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 414	394	Hyaluronidase-3		PRO_0000248203
Regions
	Domain	349 – 404	56	EGF-like
Sites
	Active site	129	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	69	1	N-linked (GlcNAc...) Potential
	Glycosylation	215	1	N-linked (GlcNAc...) Potential
	Disulfide bond	42 ↔ 328		By similarity
	Disulfide bond	205 ↔ 220		By similarity
	Disulfide bond	353 ↔ 364		By similarity
	Disulfide bond	358 ↔ 392		By similarity
	Disulfide bond	394 ↔ 403		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5REQ1-1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: CBE2FB0A6F8633A8	FASTA	414	46,114
	10 20 30 40 50 60 MTTRLGPALV LGVALCLGCG QPLPQVPERP FSVLWNVPSA HCKSRFGVHL PLNALGIIAN 70 80 90 100 110 120 RGQHFHGQNM TIFYKNQLGL YPYFGPKGTA HNGGIPQALP LDRHLALAAY QIHHSLRPGF 130 140 150 160 170 180 AGPAVLDWEE WCPLWAGNWG RRRAYQAASW AWAQQVFPDL DPQEQLYKAY TGFEQAARAL 190 200 210 220 230 240 MEDTLRVAQA LRPHGLWGFY HYPACGNGWH SMASNYTGRC HAATLARNTQ LHWLWAASSA 250 260 270 280 290 300 LFPSIYLPPR LPPAHHQAFV RHRLEEAFRV ALVGHLPVLA YVRLTHRRSG RFLSQDDLVQ 310 320 330 340 350 360 TIGVSAALGA AGVVLWGDLS LSSSEEECWH LHDYLVDTLG PYGINVTRAA MACSHQRCHG 370 380 390 400 410 HGRCARRDPG QMEAFLHLWP DGSLGDWKSF SCHCYWGWAG PTCQEPRLGP KEAV

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR857468 mRNA. Translation: CAH89756.1.
3D structure databases
HSSP	HSSP built from PDB template 1FCQ based on UniProtKB Q08169.
SMR	Q5REQ1. Positions 24-408.
ModBase	Search...
Protein family/group databases
CAZy	GH56. Glycoside Hydrolase Family 56.
Phylogenomic databases
HOVERGEN	Q5REQ1.
Enzyme and pathway databases
BRENDA	3.2.1.35. 269192.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR006210. EGF-like. IPR017430. Glyco_hydro_56_Hyaluronidase. IPR017853. Glyco_hydro_catalytic_core. IPR001968. Glycoside_hydrolase_family_56. IPR018155. Hyaluronidase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11769. Glyco_hydro_56. 1 hit.
Pfam	PF01630. Glyco_hydro_56. 1 hit. [Graphical view]
PIRSF	PIRSF038193. Hyaluronidase. 1 hit.
PRINTS	PR00846. GLHYDRLASE56.
SMART	SM00181. EGF. 1 hit. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HYAL3_PONAB

Accession

Primary (citable) accession number: Q5REQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	December 21, 2004
Last modified:	January 19, 2010
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)