Reviewed, UniProtKB/Swiss-Prot Q5REQ0 (AK1C1_PONAB)

Names and origin

Protein names

Recommended name:
    Aldo-keto reductase family 1 member C1 homolog
    EC=1.-.-.-
Alternative name(s):
    20-alpha-hydroxysteroid dehydrogenase
      Short name=20-alpha-HSD
    EC=1.1.1.149
    Indanol dehydrogenase
    EC=1.1.1.112

Gene names

Name:

AKR1C1

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	17-alpha,20-alpha-dihydroxypregn-4-en-3-one + NAD(P)+ = 17-alpha-hydroxyprogesterone + NAD(P)H. Indan-1-ol + NAD(P)+ = indanone + NAD(P)H.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	bile acid metabolic process Inferred from sequence or structural similarity. Source: UniProtKB digestion Inferred from sequence or structural similarity. Source: UniProtKB oxidation reduction Inferred from sequence or structural similarity. Source: UniProtKB protein homooligomerization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	20-alpha-hydroxysteroid dehydrogenase activity Inferred from electronic annotation. Source: EC 3-alpha-hydroxysteroid dehydrogenase (B-specific) activity Inferred from sequence or structural similarity. Source: UniProtKB bile acid binding Inferred from sequence or structural similarity. Source: UniProtKB trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	Aldo-keto reductase family 1 member C1 homolog		PRO_0000124636
Regions
	Nucleotide binding	13 – 22	10	NADP Potential
	Nucleotide binding	217 – 280	64	NADP By similarity
Sites
	Active site	55	1	Proton donor By similarity
	Binding site	117	1	Substrate By similarity
	Site	54	1	Important for substrate specificity By similarity
	Site	84	1	Lowers pKa of active site Tyr By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5REQ0-1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 5AB17BAF9B6E3D23	FASTA	323	36,932
	10 20 30 40 50 60 MDSKYQCVKL NDSHFMPVLG FGTYAPPEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ 70 80 90 100 110 120 VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLVHFPV 130 140 150 160 170 180 SVKPGEEVIP KDENGKILFD TVDLCATWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKP 190 200 210 220 230 240 GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEKWVDP NSPVLLEDPV 250 260 270 280 290 300 LCALAKKHKQ TPALIALRYQ LQRGVVVLAK SYNEQRIRQN MQVFDFQLTS EDMKTIDGLN 310 320 RNMRYLTLDI FAGPPNYPFS DEY

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR857469 mRNA. Translation: CAH89757.1.
RefSeq	NP_001124803.1.
UniGene	Pab.19423
3D structure databases
SMR	Q5REQ0. Positions 2-323.
ModBase	Search...
Genome annotation databases
GeneID	100171658.
Phylogenomic databases
HOVERGEN	Q5REQ0.
Enzyme and pathway databases
BRENDA	1.1.1.149. 269192.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AK1C1_PONAB

Accession

Primary (citable) accession number: Q5REQ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	December 21, 2004
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)