Q5REE8 (RPA2_PONAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase I subunit RPA2
Short name=RNA polymerase I subunit 2
EC=2.7.7.6

Alternative name(s):
RPA135

Gene names

Name:

POLR1B

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	1136 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits By similarity.
Subcellular location	Nucleus › nucleolus By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Cellular component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1136	1136	DNA-directed RNA polymerase I subunit RPA2		PRO_0000230309
Regions
	Zinc finger	1071 – 1102	32	C4-type Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5REE8 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 0ACF3C02941D300F	FASTA	1,136	128,497
	10 20 30 40 50 60 MDPGSRWRNL PSGPSLKHLT DPSYGIPREQ QKAALQELTR AHVESFNYAV HEGLGLAVQA 70 80 90 100 110 120 IPPFEFAFKD ERISFTILDA VISPPTVPKG TICKEVSVYP AECRGRRCTY RGKLTADISW 130 140 150 160 170 180 AVNGISKGII KQFLGYVPIM VKSKLCNLYN LPPQALIEHH EEAEEMGGYF IINGIEKVIR 190 200 210 220 230 240 MLIMPRRNFP IAMVRPKWKT RGPGYTHYGV SMHCVREEHS AVNMNLHYLE NGTVMLNFIY 250 260 270 280 290 300 RKELFFLPLG FALKALVSFS DYQIFQELIK GKEDDSFLRN SVSQMLRIVM EEGCSTQKQV 310 320 330 340 350 360 LNYLGECFRV KLNVPDWYPN EQAAEFLFNQ CICIHLKSNT EKFYMLCLMT RKLFALAKGE 370 380 390 400 410 420 CMEDNPDSLV NQEVLTPGQL FLMFLKEKLE GWLVSIKIAF DKKAQKTNVS MNTDNLMRIF 430 440 450 460 470 480 TMGIDLTKPF EYLFATGNLR SKTGLGLLQD SGLCVVADKL NFIRYLSHFR CVHRGADFAK 490 500 510 520 530 540 MRTTTVRRLL PESWGFLCPV HTPDGEPCGL MNHLTAVCEV VTQFVYTASI PALLCNLGVT 550 560 570 580 590 600 PIDGAPHRSY SECYPVLLDG VMVGWVDKEL APGIADSLRH FKVLREKRIP PWTEVVLIPM 610 620 630 640 650 660 TGKPSLYPGL FLFTTPCRLV RPVQNLELGK EELIGTMEQI FMNVAIFEDE VFAGVTTHQE 670 680 690 700 710 720 LFPHSLLSVI ANFIPFSDHN QSPRNMYQCQ MGKQTMGFPL LTYQDRSDNK LYRLQTPQSP 730 740 750 760 770 780 LVRPSMYDYY DMDNYPIGTN AIVAVISYTG YDMEDAMIVN KASWERGFAH GSVYKSEFID 790 800 810 820 830 840 LSEKIKQGDS SLVFGMKPGD PRILQKLDDD GLPFIGAKLQ YGDPYYSYLN LNTGESFVMY 850 860 870 880 890 900 YKSKENCVVD NIKVCSNDTG SGKFKCVCIT MRVPRNPTIG DKFASRHGQK GILSRLWPAE 910 920 930 940 950 960 DMPFTESGMV PDILFNPHGF PSRMTIGMLI ESMAGKSAAL HGLCHDATPF IFSEENSALE 970 980 990 1000 1010 1020 YFGEMLKAAG YNFYGTERLY SGISGLELEA DIFIGVVYYQ RLRHMVSDKF QVRTTGARDR 1030 1040 1050 1060 1070 1080 VTNQPIGGRN VQGGIRFGEM ERDALLAHGT SFFLLHDRLF NCSDRSVAHV CVKCGSLLSP 1090 1100 1110 1120 1130 LLEKPPPSWS AMRNRKYNCT LCNRSDTIDT VSVPYVFRYF VAELAAMNIK VKLDVV

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR857581 mRNA. Translation: CAH89859.1.
RefSeq	NP_001128800.1. NM_001135328.1.
UniGene	Pab.8073.
3D structure databases
ProteinModelPortal	Q5REE8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100171727.
KEGG	pon:100171727.
Organism-specific databases
CTD	84172.
Phylogenomic databases
HOVERGEN	HBG017742.
InParanoid	Q5REE8.
Family and domain databases
InterPro	IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR009674. RNA_pol_Rpa2-specific. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007647. RNA_pol_Rpb2_5. IPR007641. RNA_pol_Rpb2_7. [Graphical view]
Gene3D	G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits.
KO	K03002.
PANTHER	PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF06883. RNA_pol_Rpa2_4. 1 hit. PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 1 hit. PF04565. RNA_pol_Rpb2_3. 1 hit. PF04567. RNA_pol_Rpb2_5. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPA2_PONAB

Accession

Primary (citable) accession number: Q5REE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	December 21, 2004
Last modified:	November 16, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program