Reviewed, UniProtKB/Swiss-Prot Q5RED7 (AGK_PONAB)

Names and origin

Protein names

Recommended name:
    Acylglycerol kinase, mitochondrial
    EC=2.7.1.94
    EC=2.7.1.107
Alternative name(s):
    Multiple substrate lipid kinase
      Short name=Multi-substrate lipid kinase
      Short name=MuLK

Gene names

Name:	AGK
Synonyms:	MULK

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth By similarity.
Catalytic activity	ATP + acylglycerol = ADP + acyl-sn-glycerol 3-phosphate. ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.
Cofactor	Magnesium By similarity.
Pathway	Lipid metabolism; glycerolipid metabolism.
Subcellular location	Mitochondrion membrane By similarity.
Sequence similarities	Contains 1 DAGKc domain.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation
Gene Ontology (GO)
Biological process	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acylglycerol kinase activity Inferred from electronic annotation. Source: EC diacylglycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 31	31	Mitochondrion Potential
	Chain	32 – 422	391	Acylglycerol kinase, mitochondrial		PRO_0000252382
Regions
	Domain	58 – 199	142	DAGKc
Amino acid modifications
	Modified residue	6	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5RED7-1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: A3E092F9632A6538	FASTA	422	47,068
	10 20 30 40 50 60 MTVFFKTLRN HWKKTTAGLC LLTWGGHWLY GKHCDNLLRR AACQEAQVFG NQLIPPNAQV 70 80 90 100 110 120 KKATVFLNPA ACKGKARTLF EKNAAPILHL CGMDVTIVKT DYEGQAKKLL ELMENTDVII 130 140 150 160 170 180 VAGGDGTLQE VVTGVLRRTD EATFSKIPIG FIPLGETSSL SHTLFAESGN KVQHITDATL 190 200 210 220 230 240 AIVKGETVPL DVLQIKGEKE QPVFAMTGLR WGSFRDAGVK VSKYWYLGPL KIKAAHFFST 250 260 270 280 290 300 LKEWPQTHQA SISYTGPTER PPSEPEETPV QRPSLYRRIL RRLASYWAQP QDALSQEVSP 310 320 330 340 350 360 EVWKDVQLST IELSITTRNN QLDPTSKEDF LNICIEPDTI SKGDFITIGS RKVRNPKLHA 370 380 390 400 410 420 EGTECLQASQ CTLLIPEGAG GSFSIDSEEY EAMPVEVKLL PRKLQFFCDP RKREQMLTSP AQ

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR857592 mRNA. Translation: CAH89870.1.
RefSeq	NP_001124872.1.
UniGene	Pab.19403
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	100171735.
Organism-specific databases
CTD	100171735.
Phylogenomic databases
HOVERGEN	Q5RED7.
OMA	WYLGPLK.
Enzyme and pathway databases
BRENDA	2.7.1.107. 269192. 2.7.1.94. 269192.
Family and domain databases
InterPro	IPR001206. Diacylglycerol_kinase_cat. [Graphical view]
Pfam	PF00781. DAGK_cat. 1 hit. [Graphical view]
ProDom	PD005043. DAGKc. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS50146. DAGK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGK_PONAB

Accession

Primary (citable) accession number: Q5RED7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 17, 2006
Last sequence update:	December 21, 2004
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)