Reviewed, UniProtKB/Swiss-Prot Q5RE98 (AOFB_PONAB)

Names and origin

Protein names

Recommended name:
    Amine oxidase [flavin-containing] B
    EC=1.4.3.4
Alternative name(s):
    Monoamine oxidase type B
      Short name=MAO-B

Gene names

Name:

MAOB

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine By similarity.
Catalytic activity	RCH2NHR' + H2O + O2 = RCHO + R'NH2 + H2O2.
Cofactor	FAD By similarity.
Subunit structure	Monomer, homo- or heterodimer (containing two subunits of similar size). Each subunit contains a covalently bound flavin. Enzymatically active as monomer By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the flavin monoamine oxidase family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amine oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 520	519	Amine oxidase [flavin-containing] B		PRO_0000099862
Regions
	Topological domain	2 – 489	488	Cytoplasmic By similarity
	Transmembrane	490 – 516	27	Anchor for type IV membrane protein By similarity
	Topological domain	517 – 520	4	Mitochondrial intermembrane By similarity
	Compositional bias	36 – 52	17	Arg/Lys-rich (basic)
Sites
	Site	156	1	Important for catalytic activity By similarity
	Site	365	1	Important for catalytic activity By similarity
	Site	382	1	Important for catalytic activity By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	52	1	N6-acetyllysine By similarity
	Modified residue	397	1	S-8alpha-FAD cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5RE98-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: D68DC71B2BB6035A	FASTA	520	58,733
	10 20 30 40 50 60 MSNKCDVVVV GGGISGMAAA KLLHDSGLNV VVLEARDRVG GRTYTLRNQK VKYVDLGGSY 70 80 90 100 110 120 VGPTQNRILR LAKELGLETY KVNEVERLIH HVKGKSYPFR GPFPPVWNPI TYLDHNNFWR 130 140 150 160 170 180 TMDDMGREIP SDAPWKAPLA EEWDNMTMKE LVDKLCWTES AKQLATLFVN LCVTAETHEV 190 200 210 220 230 240 SALWFLWYVK QCGGTTRIIS TTNGGQERKF VGGSGQVSER IMDLLGDRVK LERPVIYIDQ 250 260 270 280 290 300 TRENVLVETL NHEMYEAKYV ISAIPPTLGM KIHFNPPLPM MRNQMITRVP LGSVIKCIVY 310 320 330 340 350 360 YKEPFWRKKD YCGTMIIDGE EAPVAYTLDD TKPEGNYAAI MGFILAHKAR KLARLTKEER 370 380 390 400 410 420 LKKLCELYAK VLGSPEALEP VHYEEKNWCE EQYSGGCYTT YFPPGILTQY GRVLRQPVDR 430 440 450 460 470 480 IYFAGTETAT HWSGYMEGAV EAGERAAREI LHAMGKIPED EIWQSEPESV DVPAQPITTT 490 500 510 520 FLERHLPSVP GLLRLIGLTT IFSATALGFL AHKRGLLVRV

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR857635 mRNA. Translation: CAH89909.1.
RefSeq	NP_001124895.1.
UniGene	Pab.7317
3D structure databases
SMR	Q5RE98. Positions 3-501.
ModBase	Search...
Genome annotation databases
GeneID	100171761.
Organism-specific databases
CTD	100171761.
Phylogenomic databases
HOVERGEN	Q5RE98.
Enzyme and pathway databases
BRENDA	1.4.3.4. 269192.
Family and domain databases
InterPro	IPR001613. Amineoxid_fl. IPR002937. Amino_oxidase. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
PRINTS	PR00757. AMINEOXDASEF.
ProtoNet	Search...

Entry information

Entry name

AOFB_PONAB

Accession

Primary (citable) accession number: Q5RE98

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 40 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)