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Q5RE69

- NEP_PONAB

UniProt

Q5RE69 - NEP_PONAB

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Protein
Neprilysin
Gene
MME
Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 By similarity. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers By similarity.

Catalytic activityi

Preferential cleavage of polypeptides between hydrophobic residues, particularly with Phe or Tyr at P1'.

Cofactori

Binds 1 zinc ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei103 – 1031Substrate carboxyl By similarity
Metal bindingi584 – 5841Zinc; catalytic By similarity
Active sitei585 – 5851 By similarity
Metal bindingi588 – 5881Zinc; catalytic By similarity
Metal bindingi647 – 6471Zinc; catalytic By similarity
Active sitei651 – 6511Proton donor By similarity

GO - Molecular functioni

  1. metalloendopeptidase activity Source: UniProtKB
  2. peptide binding Source: UniProtKB
  3. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. beta-amyloid metabolic process Source: UniProtKB
  2. cellular response to UV-A Source: UniProtKB
  3. cellular response to UV-B Source: UniProtKB
  4. cellular response to cytokine stimulus Source: UniProtKB
  5. creatinine metabolic process Source: UniProtKB
  6. kidney development Source: UniProtKB
  7. peptide metabolic process Source: UniProtKB
  8. proteolysis Source: UniProtKB
  9. replicative senescence Source: UniProtKB
  10. sensory perception of pain Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM13.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Neprilysin (EC:3.4.24.11)
Alternative name(s):
Atriopeptidase
Enkephalinase
Neutral endopeptidase 24.11
Short name:
NEP
Short name:
Neutral endopeptidase
Skin fibroblast elastase
Short name:
SFE
CD_antigen: CD10
Gene namesi
Name:MME
OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Taxonomic identifieri9601 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
ProteomesiUP000001595: Chromosome 3

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 2827Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei29 – 5123Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini52 – 750699Extracellular Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. axon Source: UniProtKB
  2. brush border Source: UniProtKB
  3. cytoplasm Source: UniProtKB
  4. dendrite Source: UniProtKB
  5. integral component of membrane Source: UniProtKB-KW
  6. neuron projection terminus Source: UniProtKB
  7. plasma membrane Source: UniProtKB
  8. synapse Source: UniProtKB
  9. synaptic vesicle Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 750749Neprilysin
PRO_0000319885Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine By similarity
Disulfide bondi57 ↔ 62 By similarity
Disulfide bondi80 ↔ 735 By similarity
Disulfide bondi88 ↔ 695 By similarity
Disulfide bondi143 ↔ 411 By similarity
Glycosylationi145 – 1451N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi234 ↔ 242 By similarity
Glycosylationi285 – 2851N-linked (GlcNAc...) Reviewed prediction
Glycosylationi311 – 3111N-linked (GlcNAc...) Reviewed prediction
Glycosylationi325 – 3251N-linked (GlcNAc...) Reviewed prediction
Glycosylationi335 – 3351N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi621 ↔ 747 By similarity
Glycosylationi628 – 6281N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Myristoylation is a determinant of membrane targeting By similarity.
Glycosylation at Asn-628 is necessary both for surface expression and neutral endopeptidase activity By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Myristate

Structurei

3D structure databases

ProteinModelPortaliQ5RE69.
SMRiQ5RE69. Positions 55-750.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi16 – 238Stop-transfer sequence Reviewed prediction

Sequence similaritiesi

Belongs to the peptidase M13 family.

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00650000093248.
HOGENOMiHOG000245574.
HOVERGENiHBG005554.
InParanoidiQ5R5K3.
KOiK01389.
OMAiVWCGTYR.
OrthoDBiEOG7PZRWQ.
TreeFamiTF315192.

Family and domain databases

Gene3Di3.40.390.10. 2 hits.
InterProiIPR024079. MetalloPept_cat_dom.
IPR000718. Peptidase_M13.
IPR018497. Peptidase_M13_C.
IPR008753. Peptidase_M13_N.
[Graphical view]
PANTHERiPTHR11733. PTHR11733. 1 hit.
PfamiPF01431. Peptidase_M13. 1 hit.
PF05649. Peptidase_M13_N. 1 hit.
[Graphical view]
PRINTSiPR00786. NEPRILYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5RE69-1 [UniParc]FASTAAdd to Basket

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MGKSESQMDI TDINTPKPKK KQRWTPLEIS LSVLVLLLTI IAVTMIALYA    50
TYDDGICKSS DCIKSAARLI QNMDATAEPC TDFFKYACGG WLKRNVIPET 100
SSRYGNFDIL RDELEVVLKD VLQEPKTEDI VAVQKAKTLY RSCINESAID 150
SRGGEPLLKL LPDVYGWPVA TENWEQKYGA SWTAEKAIAQ LNSKYGKKVL 200
INLFVGTDDK NSVNHVIHID QPRLGLPSRD YYECTGIYKE ACTAYVDFMI 250
SVARLIRQEE RLPIDENQLA LEMNKVMELE KEIANATAKP EDRNDPMLLY 300
KKMTLAQIQN NFSLEINGKP FSWLNFTNEI MSTVNISITN EEDVVVYAPE 350
YLTKLKPILT KYSARDLQNL MSWRFIMDLV SSLSRTYKES RNAFRKALYG 400
TTSETATWRR CANYVNGNME NAVGRLYVEA AFAGESKHVV EDLIAQIREV 450
FIQTLDDLTW MDAETKKRAE EKALAIKERI GYPDDIVSND NKLNNEYLEL 500
NYKEDEYFEN IIQNLKFSQS KQLKKLREKV DKDEWISGAA VVNAFYSSGR 550
NQIVFPAGIL QPPFFSAQQS NSLNYGGIGM VIGHEITHGF DDNGRNFNKD 600
GDLVDWWTQQ SASNFKEQSQ CMVYQYGNFS WDLAGGQHLN GINTLGENIA 650
DNGGLGQAYR AYQNYIKKNG EEKLLPGLDL NHKQLFFLNF AQVWCGTYRP 700
EYAVNSIKTD VHSPGNFRII GTLQNSAEFS EAFHCRKNSY MNPEKKCRVW 750
Length:750
Mass (Da):85,514
Last modified:February 26, 2008 - v2
Checksum:iC4D1216E8361352F
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti115 – 1151E → G in CAH89405. 1 Publication
Sequence conflicti175 – 1751E → K in CAH89405. 1 Publication
Sequence conflicti523 – 5231L → P in CAH89938. 1 Publication
Sequence conflicti681 – 6811N → S in CAH92963. 1 Publication
Sequence conflicti729 – 7291F → C in CAH92963. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR857100 mRNA. Translation: CAH89405.1.
CR857668 mRNA. Translation: CAH89938.1.
CR860855 mRNA. Translation: CAH92963.1.
RefSeqiNP_001126748.1. NM_001133276.1.

Genome annotation databases

EnsembliENSPPYT00000016535; ENSPPYP00000015904; ENSPPYG00000014224.
GeneIDi100173750.
KEGGipon:100173750.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR857100 mRNA. Translation: CAH89405.1 .
CR857668 mRNA. Translation: CAH89938.1 .
CR860855 mRNA. Translation: CAH92963.1 .
RefSeqi NP_001126748.1. NM_001133276.1.

3D structure databases

ProteinModelPortali Q5RE69.
SMRi Q5RE69. Positions 55-750.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M13.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSPPYT00000016535 ; ENSPPYP00000015904 ; ENSPPYG00000014224 .
GeneIDi 100173750.
KEGGi pon:100173750.

Organism-specific databases

CTDi 4311.

Phylogenomic databases

GeneTreei ENSGT00650000093248.
HOGENOMi HOG000245574.
HOVERGENi HBG005554.
InParanoidi Q5R5K3.
KOi K01389.
OMAi VWCGTYR.
OrthoDBi EOG7PZRWQ.
TreeFami TF315192.

Family and domain databases

Gene3Di 3.40.390.10. 2 hits.
InterProi IPR024079. MetalloPept_cat_dom.
IPR000718. Peptidase_M13.
IPR018497. Peptidase_M13_C.
IPR008753. Peptidase_M13_N.
[Graphical view ]
PANTHERi PTHR11733. PTHR11733. 1 hit.
Pfami PF01431. Peptidase_M13. 1 hit.
PF05649. Peptidase_M13_N. 1 hit.
[Graphical view ]
PRINTSi PR00786. NEPRILYSIN.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. The German cDNA consortium
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiNEP_PONAB
AccessioniPrimary (citable) accession number: Q5RE69
Secondary accession number(s): Q5R5K3, Q5RFQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: February 26, 2008
Last modified: April 16, 2014
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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