Reviewed, UniProtKB/Swiss-Prot Q5RE60 (AOFA_PONAB)

Names and origin

Protein names

Recommended name:
    Amine oxidase [flavin-containing] A
    EC=1.4.3.4
Alternative name(s):
    Monoamine oxidase type A
      Short name=MAO-A

Gene names

Name:

MAOA

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	527 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine By similarity.
Catalytic activity	RCH2NHR' + H2O + O2 = RCHO + R'NH2 + H2O2.
Cofactor	FAD By similarity.
Subunit structure	Monomer, homo- or heterodimer (containing two subunits of similar size). Each subunit contains a covalently bound flavin. Enzymatically active as monomer By similarity.
Subcellular location	Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side By similarity.
Sequence similarities	Belongs to the flavin monoamine oxidase family.

Ontologies

Keywords
Biological process	Catecholamine metabolism Neurotransmitter degradation
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	catecholamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW neurotransmitter catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amine oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 527	527	Amine oxidase [flavin-containing] A		PRO_0000099853
Regions
	Topological domain	1 – 497	497	Cytoplasmic By similarity
	Transmembrane	498 – 518	21	Anchor for type IV membrane protein By similarity
	Topological domain	519 – 527	9	Mitochondrial intermembrane By similarity
	Region	520 – 522	3	Interaction with membrane phospholipid headgroups By similarity
Sites
	Site	335	1	Important for substrate specificity By similarity
	Site	374	1	Important for catalytic activity By similarity
Amino acid modifications
	Modified residue	406	1	S-8alpha-FAD cysteine By similarity
Experimental info
	Sequence conflict	382	1	G → E in CAH89637. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q5RE60-1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 8CC74D22F867CE8F	FASTA	527	59,666
	10 20 30 40 50 60 MENQEKASIA GHMFDVVVIG GGISGLSAAK LLTEYGVSVL VLEARDRVGG RTYTIRNEHV 70 80 90 100 110 120 DYVDVGGAYV GPTQNRILRL SKELGIETYK VNVSERLVQY VKGKTYPFRG AFPPVWNPIA 130 140 150 160 170 180 YLDYNNLWRT IDNMGKEIPA DAPWEAQHAD EWDKMTMKEL IDKICWTKTA RRFAYLFVNI 190 200 210 220 230 240 NVTSEPHEVS ALWFLWYVKQ CGGTTRIFSV TNGGQERKFV GGSGQVSERI MDLLGDQVKL 250 260 270 280 290 300 NHPVTHVDQS SNNIIIETLN HEHYECKYVI NAIPPTLTAK IHFRPELPAE RNQLIQRLPM 310 320 330 340 350 360 GAIIKCMMYY KEAFWKKKDY CGCMIIEDED APISITLDDT KPDGSLPAIM GFILARKADR 370 380 390 400 410 420 LAKLHKEIRK KKICELYAKV LGSQEALHPV HYEEKNWCEE QYSGGCYTAY FPPGIMTQYG 430 440 450 460 470 480 RVIRQPVGRI FFAGTETATK WSGYMEGAVE AGERAAREVL NGLGKVTEKD IWVQEPESKD 490 500 510 520 VPAVEITHTF WERNLPSVSG LLKIIGFSTS VTALGFVLYK YKLLPRS

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR857341 mRNA. Translation: CAH89637.1. CR857677 mRNA. Translation: CAH89947.1.
RefSeq	NP_001124913.1.
UniGene	Pab.18225
3D structure databases
SMR	Q5RE60. Positions 23-509.
ModBase	Search...
Genome annotation databases
GeneID	100171783.
Organism-specific databases
CTD	100171783.
Phylogenomic databases
HOVERGEN	Q5RE60.
OMA	YLFVNIN.
Enzyme and pathway databases
BRENDA	1.4.3.4. 269192.
Family and domain databases
InterPro	IPR001613. Amineoxid_fl. IPR002937. Amino_oxidase. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
PRINTS	PR00757. AMINEOXDASEF.
ProtoNet	Search...

Entry information

Entry name

AOFA_PONAB

Accession

Primary (citable) accession number: Q5RE60
Secondary accession number(s): Q5RF20

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	December 21, 2004
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)