Reviewed, UniProtKB/Swiss-Prot Q5RDH5 (AAPK1_PONAB)

Names and origin

Protein names

Recommended name:
    5'-AMP-activated protein kinase catalytic subunit alpha-1
      Short name=AMPK alpha-1 chain
    EC=2.7.11.1

Gene names

Name:

PRKAA1

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	550 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-174 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio By similarity.
Subunit structure	Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits. Interacts with FNIP1 and FNIP2 By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Fatty acid biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 550	550	5'-AMP-activated protein kinase catalytic subunit alpha-1		PRO_0000085592
Regions
	Domain	18 – 270	253	Protein kinase
	Nucleotide binding	24 – 32	9	ATP By similarity
Sites
	Active site	141	1	Proton acceptor By similarity
	Binding site	47	1	ATP By similarity
Amino acid modifications
	Modified residue	174	1	Phosphothreonine; by STK11 By similarity
	Modified residue	175	1	Phosphoserine By similarity
	Modified residue	260	1	Phosphothreonine By similarity
	Modified residue	347	1	Phosphoserine By similarity
	Modified residue	373	1	Phosphothreonine By similarity
	Modified residue	433	1	Phosphotyrosine By similarity
	Modified residue	477	1	Phosphoserine By similarity
	Modified residue	481	1	Phosphothreonine By similarity
	Modified residue	487	1	Phosphoserine By similarity
	Modified residue	499	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5RDH5-1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: FC2F12DDD858B1C2	FASTA	550	62,836
	10 20 30 40 50 60 MATAEKQKHD GRVRIGHYIL GDTLGVGTFG KVKVGKHELT GHKVAVKILN RQKIRSLDVV 70 80 90 100 110 120 GKIRREIQNL KLFRHPHIIK LYQVISTPSD IFMVMEYVSG GELFDYICKN GRLDEKESRR 130 140 150 160 170 180 LFQQILSGVD YCHRHMVVHR DLKPENVLLD AHMNAKIADF GLSNMMSDGE FLRTSCGSPN 190 200 210 220 230 240 YAAPEVISGR LYAGPEVDIW SSGVILYALL CGTLPFDDDH VPTLFKKICD GIFYTPQYLN 250 260 270 280 290 300 PSVISLLKHM LQVDPMKRAT IKDIREHEWF KQDLPKYLFP EDPSYSSTMI DDEALKEVCE 310 320 330 340 350 360 KFECSEEEVL SCLYNRNHQD PLAVAYHLII DNRRIMNEAK DFYLATSPPD SFLDDHHLTR 370 380 390 400 410 420 PHPERVPFLV AETPRARHTL DELNPQKSKH QGVRKAKWHL GIRSQSRPND IMAEVCRAIK 430 440 450 460 470 480 QLDYEWKVVN PYYLRVRRKN PVTSTYSKMS LQLYQVDSRT YLLDFRSIDD EITEAKSGTA 490 500 510 520 530 540 TPQRSGSVSN YRSCQRSDSD AEAQGKSSEV SLTSSVTSLD SSPVDLTPRP GSHTIEFFEM 550 CANLIKILAQ

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR857935 mRNA. Translation: CAH90182.1.
RefSeq	NP_001127249.1.
3D structure databases
SMR	Q5RDH5. Positions 12-280.
ModBase	Search...
Genome annotation databases
GeneID	100174304.
Phylogenomic databases
HOVERGEN	Q5RDH5.
Family and domain databases
InterPro	IPR015741. AMPK. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
PANTHER	PTHR22982:SF61. AMPK. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AAPK1_PONAB

Accession

Primary (citable) accession number: Q5RDH5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	December 21, 2004
Last modified:	November 25, 2008
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)