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Protein

Cleavage stimulation factor subunit 2

Gene

CSTF2

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit is directly involved in the binding to pre-mRNAs (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cleavage stimulation factor subunit 2
Alternative name(s):
CF-1 64 kDa subunit
Cleavage stimulation factor 64 kDa subunit
Short name:
CSTF 64 kDa subunit
Short name:
CstF-64
Gene namesi
Name:CSTF2
OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Taxonomic identifieri9601 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
Proteomesi
  • UP000001595 Componenti: Unplaced

Subcellular locationi

  • Nucleus By similarity

  • Note: Localized with DDX1 in cleavage bodies.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 577577Cleavage stimulation factor subunit 2PRO_0000081533Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141PhosphoserineBy similarity
Cross-linki189 – 189Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei518 – 5181PhosphoserineBy similarity
Modified residuei524 – 5241PhosphoserineBy similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Interactioni

Subunit structurei

The CSTF complex is composed of CSTF1 (50 kDa subunit), CSTF2 (64 kDa subunit) and CSTF3 (77 kDa subunit). CSTF2 directly interacts with CSTF3, SYMPK and RPO2TC1. Interacts with HSF1 in heat-stressed cells (By similarity). Interacts with CPSF2, CPSF3 and FIP1L1. Interacts with DDX1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9601.ENSPPYP00000022995.

Structurei

3D structure databases

ProteinModelPortaliQ5RDA3.
SMRiQ5RDA3. Positions 8-111, 529-577.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 9479RRMPROSITE-ProRule annotationAdd
BLAST
Repeati410 – 41451; approximate
Repeati415 – 41952
Repeati420 – 42453
Repeati425 – 42954; approximate
Repeati430 – 43455; approximate
Repeati435 – 43956
Repeati440 – 44457
Repeati445 – 44958
Repeati450 – 45459
Repeati455 – 459510; approximate
Repeati460 – 464511
Repeati465 – 469512; approximate

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni108 – 248141Interactions with CSTF3 and SYMPKBy similarityAdd
BLAST
Regioni410 – 4696012 X 5 AA tandem repeats of M-E-A-R-[AG]Add
BLAST
Regioni514 – 57764Interaction with RPO2TC1By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi198 – 409212Gly/Pro-richAdd
BLAST
Compositional biasi470 – 52657Gly/Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0108. Eukaryota.
ENOG410XQBV. LUCA.
HOVERGENiHBG051145.
InParanoidiQ5RDA3.
KOiK14407.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR033105. CSTF2.
IPR025742. CSTF2_hinge.
IPR026896. CSTF_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR23139:SF57. PTHR23139:SF57. 1 hit.
PfamiPF14327. CSTF2_hinge. 1 hit.
PF14304. CSTF_C. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5RDA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGLTVRDPA VDRSLRSVFV GNIPYEATEE QLKDIFSEVG PVVSFRLVYD
60 70 80 90 100
RETGKPKGYG FCEYQDQETA LSAMRNLNGR EFSGRALRVD NAASEKNKEE
110 120 130 140 150
LKSLGTGAPV IESPYGETIS PEDAPESISK AVASLPPEQM FELMKQMKLC
160 170 180 190 200
VQNSPQEARN MLLQNPQLAY ALLQAQVVMR IVDPEIALKI LHRQTNIPTL
210 220 230 240 250
IAGNPQPVHG AGPGSGSNVS MNQQNPQAPQ AQSLGGMHVN GAPPLMQASM
260 270 280 290 300
QGGVPAPGQI PAAVTGPGPG SLAPGGGMQA QVGMPGSGPV SMERGQVPMQ
310 320 330 340 350
DPRAAMQRGS LPANVPTPRG LLGDAPNDPR GGTLLSVTGE VEPRGYLGPP
360 370 380 390 400
HQGPPMHHVP GHESRGPPPH ELRGGPLPEP RPLMAEPRGP MLDQRGPPLD
410 420 430 440 450
GRGGRDPRGI DARGMEARAM EARGLDARGL EARAMEARAM EARAMEARAM
460 470 480 490 500
EARAMEVRGM EARGMDTRGP VPGPRGPIPS GMQGPSPINM GAVVPQGSRQ
510 520 530 540 550
VPVMQGTGLQ GASIQGGSQP GGFSPGQNQV TPQDHEKAAL IMQVLQLTAD
560 570
QIAMLPPEQR QSILILKEQI QKSTGAP
Length:577
Mass (Da):60,923
Last modified:December 21, 2004 - v1
Checksum:i428F2FFDBDE8DAA8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR858012 mRNA. Translation: CAH90254.1.
RefSeqiNP_001125111.1. NM_001131639.2.
UniGeneiPab.17359.

Genome annotation databases

GeneIDi100171993.
KEGGipon:100171993.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR858012 mRNA. Translation: CAH90254.1.
RefSeqiNP_001125111.1. NM_001131639.2.
UniGeneiPab.17359.

3D structure databases

ProteinModelPortaliQ5RDA3.
SMRiQ5RDA3. Positions 8-111, 529-577.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9601.ENSPPYP00000022995.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100171993.
KEGGipon:100171993.

Organism-specific databases

CTDi1478.

Phylogenomic databases

eggNOGiKOG0108. Eukaryota.
ENOG410XQBV. LUCA.
HOVERGENiHBG051145.
InParanoidiQ5RDA3.
KOiK14407.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR033105. CSTF2.
IPR025742. CSTF2_hinge.
IPR026896. CSTF_C.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR23139:SF57. PTHR23139:SF57. 1 hit.
PfamiPF14327. CSTF2_hinge. 1 hit.
PF14304. CSTF_C. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCSTF2_PONAB
AccessioniPrimary (citable) accession number: Q5RDA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: December 21, 2004
Last modified: July 6, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.