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Protein

Cdc42-interacting protein 4

Gene

TRIP10

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Also acts as a link between CDC42 signaling and regulation of the actin cytoskeleton. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also enhances actin polymerization in the vicinity of membrane tubules by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization and dynamin may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling. May be required for the lysosomal retention of FASLG/FASL (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei166 – 1661Mediates end-to-end attachment of dimersBy similarity

GO - Molecular functioni

  1. lipid binding Source: UniProtKB-KW

GO - Biological processi

  1. endocytosis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cdc42-interacting protein 4
Alternative name(s):
Thyroid receptor-interacting protein 10
Short name:
TR-interacting protein 10
Short name:
TRIP-10
Gene namesi
Name:TRIP10
Synonyms:CIP4
OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Taxonomic identifieri9601 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
ProteomesiUP000001595 Componenti: Unplaced

Subcellular locationi

  1. Cytoplasmcytoskeleton By similarity
  2. Cytoplasmcell cortex By similarity
  3. Lysosome By similarity
  4. Golgi apparatus By similarity
  5. Cell membrane By similarity
  6. Cell projectionphagocytic cup By similarity

  7. Note: Localizes to cortical regions coincident with F-actin, to lysosomes and to sites of phagocytosis in macrophages. Also localizes to the Golgi, and this requires AKAP9. Translocates to the plasma membrane in response to insulin stimulation, and this may require active RHOQ (By similarity).By similarity

GO - Cellular componenti

  1. cell cortex Source: UniProtKB-SubCell
  2. cell projection Source: UniProtKB-KW
  3. cytoskeleton Source: UniProtKB-SubCell
  4. Golgi apparatus Source: UniProtKB-SubCell
  5. lysosome Source: UniProtKB-SubCell
  6. phagocytic cup Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 601601Cdc42-interacting protein 4PRO_0000261440Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei296 – 2961PhosphoserineBy similarity
Modified residuei298 – 2981PhosphoserineBy similarity
Modified residuei299 – 2991PhosphoserineBy similarity
Modified residuei335 – 3351PhosphoserineBy similarity
Modified residuei351 – 3511PhosphoserineBy similarity
Modified residuei482 – 4821PhosphoserineBy similarity
Modified residuei500 – 5001PhosphoserineBy similarity

Post-translational modificationi

Tyrosine phosphorylated. Also phosphorylated by PKA (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ5RCJ1.

Interactioni

Subunit structurei

Homodimerizes, the dimers can polymerize end-to-end to form filamentous structures. Interacts with AKAP9, ARHGAP17, DAAM1, DIAPH1, DIAPH2, DNM1, FASLG/FASL, GAPVD1, LYN, microtubules, PDE6G, SRC and WAS/WASP. Interacts with the ligand binding domain of the thyroid receptor (TR) in the presence of thyroid hormone. May interact with CTNNB1 and HD/HTT (By similarity). Interacts specifically with GTP-bound CDC42 and RHOQ. Interacts with DNM2 and WASL (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ5RCJ1.
SMRiQ5RCJ1. Positions 10-288, 544-599.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 264264F-BARPROSITE-ProRule annotationAdd
BLAST
Repeati405 – 48177REMAdd
BLAST
Domaini540 – 60162SH3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 117117Required for translocation to the plasma membrane in response to insulin, podosome formation and interaction with AKAP9 and microtubulesBy similarityAdd
BLAST
Regioni293 – 601309Interaction with PDE6GBy similarityAdd
BLAST
Regioni293 – 537245Interaction with CDC42By similarityAdd
BLAST
Regioni471 – 601131Required for interaction with FASLG and localization to lysosomesBy similarityAdd
BLAST
Regioni487 – 54155Interaction with DNM2 and WASLBy similarityAdd
BLAST
Regioni529 – 60173Interaction with DNM1 and WASLBy similarityAdd
BLAST
Regioni538 – 60164Required for podosome formationBy similarityAdd
BLAST
Regioni544 – 60158Interaction with WASBy similarityAdd
BLAST
Regioni546 – 60156Interaction with ARHGAP17, DAAM1, DIAPH1 and DIAPH2By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili67 – 259193By similarityAdd
BLAST
Coiled coili388 – 48194By similarityAdd
BLAST

Domaini

The F-BAR domain binds the phospholipid membrane with its concave surface. The end-to-end polymerization of dimers of these domains provides a curved surface that fits best membranes with around 600 A diameter, and may drive tubulation (By similarity).By similarity

Sequence similaritiesi

Belongs to the FNBP1 family.Curated
Contains 1 F-BAR domain.PROSITE-ProRule annotation
Contains 1 REM (Hr1) repeat.Curated
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

HOVERGENiHBG002489.
InParanoidiQ5RCJ1.
KOiK07196.

Family and domain databases

InterProiIPR028498. CIP4.
IPR001060. FCH_dom.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR12602:SF7. PTHR12602:SF7. 1 hit.
PfamiPF00611. FCH. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51741. F_BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5RCJ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDWGTELWDQ FEVLERHTQW GLDLLDRYVK FVKERTEVEQ AYAKQLRSLV
60 70 80 90 100
KKYLPKRPAK DDPESKFSQQ QSFVQILQEV NDFAGQRELV AENLSVRVCL
110 120 130 140 150
ELTKYSQEMK QERKMHFQEG RRAQQQLENG FKQLENSKRK FERDCREAEK
160 170 180 190 200
AAQTAERLDQ DINATKADVE KAKQQAHLRS HMAEESKNEY AAQLQRFNRD
210 220 230 240 250
QAHFYFSQMP QIFDKLQDMD ERRATRLGAG YGLLSEAELE VVPIIAKCLE
260 270 280 290 300
GMKVAANAVD PKNDSQVLIE LHKSGFARPG DVEFEDFSQP MNRAPSDSSL
310 320 330 340 350
GTPSDGRPEL RGPGRSRTKR WPFGKKNKPR PPPLSPLGGP VPSALPNGPP
360 370 380 390 400
SPRSGRDPLA ILSEISKSVK PRLASFRSLR GSRGTVVTED FSHLPPEQQR
410 420 430 440 450
KRLQQQLEER SRELQKEVDQ REALKKMKDV YEKTPQMGDP ASLEPQITET
460 470 480 490 500
LSNIERLKLE VQKYEAWLAE AESRVLSNRG DSLSRHARPP DPPTSAPPDS
510 520 530 540 550
SSNSASQDTK ESSEEPPSEE SQDTPIYTEF DEDFEEEPTS PIGHCVAIYH
560 570 580 590 600
FEGSSEGTIS MAEGEDLSLM EEDKGDGWTR VRRKEGGEGY VPTSYLRVTL

N
Length:601
Mass (Da):68,403
Last modified:December 21, 2004 - v1
Checksum:i40CB45B55D79E8FA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR858279 mRNA. Translation: CAH90516.1.
RefSeqiNP_001125273.1. NM_001131801.1.
UniGeneiPab.12665.

Genome annotation databases

GeneIDi100172170.
KEGGipon:100172170.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR858279 mRNA. Translation: CAH90516.1.
RefSeqiNP_001125273.1. NM_001131801.1.
UniGeneiPab.12665.

3D structure databases

ProteinModelPortaliQ5RCJ1.
SMRiQ5RCJ1. Positions 10-288, 544-599.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ5RCJ1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100172170.
KEGGipon:100172170.

Organism-specific databases

CTDi9322.

Phylogenomic databases

HOVERGENiHBG002489.
InParanoidiQ5RCJ1.
KOiK07196.

Family and domain databases

InterProiIPR028498. CIP4.
IPR001060. FCH_dom.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR12602:SF7. PTHR12602:SF7. 1 hit.
PfamiPF00611. FCH. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51741. F_BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. The German cDNA consortium
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.

Entry informationi

Entry nameiCIP4_PONAB
AccessioniPrimary (citable) accession number: Q5RCJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: December 21, 2004
Last modified: March 4, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.