Q5RCJ1 (CIP4_PONAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cdc42-interacting protein 4

Alternative name(s):
Thyroid receptor-interacting protein 10
Short name=TR-interacting protein 10
Short name=TRIP-10

Gene names

Name:	TRIP10
Synonyms:	CIP4

Organism

Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) [Reference proteome]

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Ponginae › Pongo

Protein attributes

Sequence length	601 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Also acts as a link between CDC42 signaling and regulation of the actin cytoskeleton. Binds to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promotes membrane invagination and the formation of tubules. Also enhances actin polymerization in the vicinity of membrane tubules by recruiting WASL/N-WASP which in turn activates the Arp2/3 complex. Actin polymerization and dynamin may promote the fission of membrane tubules to form endocytic vesicles. Required for the formation of podosomes, actin-rich adhesion structures specific to monocyte-derived cells. Required for translocation of GLUT4 to the plasma membrane in response to insulin signaling. May be required for the lysosomal retention of FASLG/FASL By similarity.
Subunit structure	Homodimerizes, the dimers can polymerize end-to-end to form filamentous structures. Interacts with AKAP9, ARHGAP17, DAAM1, DIAPH1, DIAPH2, DNM1, FASLG/FASL, GAPVD1, LYN, microtubules, PDE6G, SRC and WAS/WASP. Interacts with the ligand binding domain of the thyroid receptor (TR) in the presence of thyroid hormone. May interact with CTNNB1 and HD/HTT By similarity. Interacts specifically with GTP-bound CDC42 and RHOQ. Interacts with DNM2 and WASL By similarity.
Subcellular location	Cytoplasm › cytoskeleton By similarity. Cytoplasm › cell cortex By similarity. Lysosome By similarity. Golgi apparatus By similarity. Cell membrane By similarity. Cell projection › phagocytic cup By similarity. Note: Localizes to cortical regions coincident with F-actin, to lysosomes and to sites of phagocytosis in macrophages. Also localizes to the Golgi, and this requires AKAP9. Translocates to the plasma membrane in response to insulin stimulation, and this may require active RHOQ By similarity.
Domain	The F-BAR domain binds the phospholipid membrane with its concave surface. The end-to-end polymerization of dimers of these domains provides a curved surface that fits best membranes with around 600 A diameter, and may drive tubulation By similarity.
Post-translational modification	Tyrosine phosphorylated. Also phosphorylated by PKA By similarity.
Sequence similarities	Belongs to the FNBP1 family. Contains 1 FCH domain. Contains 1 REM (Hr1) repeat. Contains 1 SH3 domain.

Ontologies

Keywords
Biological process	Endocytosis
Cellular component	Cell membrane Cell projection Cytoplasm Cytoskeleton Golgi apparatus Lysosome Membrane
Domain	Coiled coil SH3 domain
Ligand	Lipid-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endocytosis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell cell cortex Inferred from electronic annotation. Source: UniProtKB-SubCell cell projection Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell phagocytic cup Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	lipid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 601	601	Cdc42-interacting protein 4		PRO_0000261440
Regions
	Domain	1 – 65	65	FCH
	Repeat	405 – 481	77	REM
	Domain	540 – 601	62	SH3
	Region	1 – 288	288	F-BAR domain By similarity
	Region	1 – 117	117	Required for translocation to the plasma membrane in response to insulin, podosome formation and interaction with AKAP9 and microtubules By similarity
	Region	293 – 601	309	Interaction with PDE6G By similarity
	Region	293 – 537	245	Interaction with CDC42 By similarity
	Region	471 – 601	131	Required for interaction with FASLG and localization to lysosomes By similarity
	Region	487 – 541	55	Interaction with DNM2 and WASL By similarity
	Region	529 – 601	73	Interaction with DNM1 and WASL By similarity
	Region	538 – 601	64	Required for podosome formation By similarity
	Region	544 – 601	58	Interaction with WAS By similarity
	Region	546 – 601	56	Interaction with ARHGAP17, DAAM1, DIAPH1 and DIAPH2 By similarity
	Coiled coil	67 – 259	193	By similarity
	Coiled coil	388 – 481	94	By similarity
Sites
	Site	166	1	Mediates end-to-end attachment of dimers By similarity
Amino acid modifications
	Modified residue	296	1	Phosphoserine By similarity
	Modified residue	299	1	Phosphoserine By similarity
	Modified residue	335	1	Phosphoserine By similarity
	Modified residue	351	1	Phosphoserine By similarity
	Modified residue	482	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5RCJ1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 40CB45B55D79E8FA	FASTA	601	68,403
	10 20 30 40 50 60 MDWGTELWDQ FEVLERHTQW GLDLLDRYVK FVKERTEVEQ AYAKQLRSLV KKYLPKRPAK 70 80 90 100 110 120 DDPESKFSQQ QSFVQILQEV NDFAGQRELV AENLSVRVCL ELTKYSQEMK QERKMHFQEG 130 140 150 160 170 180 RRAQQQLENG FKQLENSKRK FERDCREAEK AAQTAERLDQ DINATKADVE KAKQQAHLRS 190 200 210 220 230 240 HMAEESKNEY AAQLQRFNRD QAHFYFSQMP QIFDKLQDMD ERRATRLGAG YGLLSEAELE 250 260 270 280 290 300 VVPIIAKCLE GMKVAANAVD PKNDSQVLIE LHKSGFARPG DVEFEDFSQP MNRAPSDSSL 310 320 330 340 350 360 GTPSDGRPEL RGPGRSRTKR WPFGKKNKPR PPPLSPLGGP VPSALPNGPP SPRSGRDPLA 370 380 390 400 410 420 ILSEISKSVK PRLASFRSLR GSRGTVVTED FSHLPPEQQR KRLQQQLEER SRELQKEVDQ 430 440 450 460 470 480 REALKKMKDV YEKTPQMGDP ASLEPQITET LSNIERLKLE VQKYEAWLAE AESRVLSNRG 490 500 510 520 530 540 DSLSRHARPP DPPTSAPPDS SSNSASQDTK ESSEEPPSEE SQDTPIYTEF DEDFEEEPTS 550 560 570 580 590 600 PIGHCVAIYH FEGSSEGTIS MAEGEDLSLM EEDKGDGWTR VRRKEGGEGY VPTSYLRVTL N

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR858279 mRNA. Translation: CAH90516.1.
RefSeq	NP_001125273.1. NM_001131801.1.
UniGene	Pab.12665.
3D structure databases
ProteinModelPortal	Q5RCJ1.
SMR	Q5RCJ1. Positions 10-288, 544-599.
ModBase	Search...
Proteomic databases
PRIDE	Q5RCJ1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100172170.
KEGG	pon:100172170.
Organism-specific databases
CTD	9322.
Phylogenomic databases
HOVERGEN	HBG002489.
InParanoid	Q5RCJ1.
KO	K07196.
Family and domain databases
InterPro	IPR001060. FCH_dom. IPR001452. SH3_domain. [Graphical view]
Pfam	PF00611. FCH. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
SMART	SM00055. FCH. 1 hit. SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF50044. SH3. 1 hit.
PROSITE	PS50133. FCH. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CIP4_PONAB

Accession

Primary (citable) accession number: Q5RCJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	December 21, 2004
Last modified:	May 29, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program