Reviewed, UniProtKB/Swiss-Prot Q5RCE2 (STT3A_PONAB)

Names and origin

Protein names

Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A
      Short name=Oligosaccharyl transferase subunit STT3A
      Short name=STT3-A
    EC=2.4.1.119

Gene names

Name:

STT3A

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	705 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3A seems to be involved in complex substrate specificity By similarity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the STT3 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 705	705	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A		PRO_0000326557
Regions
	Topological domain	1 – 17	17	Cytoplasmic Potential
	Transmembrane	18 – 38	21	Potential
	Topological domain	39 – 114	76	Lumenal Potential
	Transmembrane	115 – 135	21	Potential
	Topological domain	136 – 169	34	Cytoplasmic Potential
	Transmembrane	170 – 190	21	Potential
	Topological domain	191 – 208	18	Lumenal Potential
	Transmembrane	209 – 229	21	Potential
	Topological domain	230 – 235	6	Cytoplasmic Potential
	Transmembrane	236 – 259	24	Potential
	Topological domain	260 – 300	41	Lumenal Potential
	Transmembrane	301 – 321	21	Potential
	Topological domain	322 – 359	38	Cytoplasmic Potential
	Transmembrane	360 – 380	21	Potential
	Topological domain	381 – 383	3	Lumenal Potential
	Transmembrane	384 – 404	21	Potential
	Topological domain	405	1	Cytoplasmic Potential
	Transmembrane	406 – 426	21	Potential
	Topological domain	427 – 453	27	Lumenal Potential
	Transmembrane	454 – 474	21	Potential
	Topological domain	475 – 705	231	Lumenal Potential
Amino acid modifications
	Glycosylation	381	1	N-linked (GlcNAc...) Potential
	Glycosylation	537	1	N-linked (GlcNAc...) Potential
	Glycosylation	544	1	N-linked (GlcNAc...) Potential
	Glycosylation	548	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5RCE2-1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 71426CA5598B51C4	FASTA	705	80,530
	10 20 30 40 50 60 MTKFGFLRLS YEKQDTLLKL LILSMAAVLS FSTRLFAVLR FESVIHEFDP YFNYRTTRFL 70 80 90 100 110 120 AEEGFYKFHN WFDDRAWYPL GRIIGGTIYP GLMITSAAIY HVLHFFHITI DIRNVCVFLA 130 140 150 160 170 180 PLFSSFTTIV TYHLTKELKD AGAGLLAAAM IAVVPGYISR SVAGSYDNEG IAIFCMLLTY 190 200 210 220 230 240 YMWIKAVKTG SICWAAKCAL AYFYMVSSWG GYVFLINLIP LHVLVLMLTG RFSHRIYVAY 250 260 270 280 290 300 CTVYCLGTIL SMQISFVGFQ PVLSSEHMAA FGVFGLCQIH AFVDYLRSKL NPQQFEVLFR 310 320 330 340 350 360 SVISLVGFVL LTVGALLMLT GKISPWTGRF YSLLDPSYAK NNIPIIASVS EHQPTTWSSY 370 380 390 400 410 420 YFDLQLLVFM FPVGLYYCFS NLSDARIFII MYGVTSMYFS AVMVRLMLVL APVMCILSGI 430 440 450 460 470 480 GVSQVLSTYM KNLDISRPDK KSKKQQDSTY PIKNEVASGM ILVMAFFLIT YTFHSTWVTS 490 500 510 520 530 540 EAYSSPSIVL SARGGDGSRI IFDDFREAYY WLRHNTPEDA KVMSWWDYGY QITAMANRTI 550 560 570 580 590 600 LVDNNTWNNT HISRVGQAMA STEEKAYEIM RELDVSYVLV IFGGLTGYSS DDINKFLWMV 610 620 630 640 650 660 RIGGSTDTGK HIKENDYYTP TGEFRVDREG SPVLLNCLMY KMCYYRFGQV YTEAKRPPGF 670 680 690 700 DRVRNAEIGN KDFELDVLEE AYTTEHWLVR IYKVKDLDNR GLSRT

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR858329 mRNA. Translation: CAH90565.1.
RefSeq	NP_001127302.1.
UniGene	Pab.2037
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT66. Glycosyltransferase Family 66.
Genome annotation databases
GeneID	100174363.
Organism-specific databases
CTD	100174363.
Phylogenomic databases
HOVERGEN	Q5RCE2.
Enzyme and pathway databases
BRENDA	2.4.1.119. 269192.
Family and domain databases
InterPro	IPR003674. Oligo_trans_STT3. [Graphical view]
Pfam	PF02516. STT3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

STT3A_PONAB

Accession

Primary (citable) accession number: Q5RCE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	December 21, 2004
Last modified:	October 13, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)