Reviewed, UniProtKB/Swiss-Prot Q5RC71 (HXK1_PONAB)

Names and origin

Protein names

Recommended name:
    Hexokinase-1
    EC=2.7.1.1
Alternative name(s):
    Hexokinase type I
      Short name=HK I

Gene names

Name:

HK1

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	917 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + D-hexose = ADP + D-hexose 6-phosphate.
Enzyme regulation	Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P By similarity.
Pathway	Carbohydrate metabolism; hexose metabolism.
Subunit structure	Monomer By similarity.
Domain	The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule By similarity.
Miscellaneous	In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).
Sequence similarities	Belongs to the hexokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hexokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 917	917	Hexokinase-1		PRO_0000286047
Regions
	Nucleotide binding	84 – 89	6	ATP 1 Potential
	Nucleotide binding	425 – 426	2	ATP 1 By similarity
	Nucleotide binding	532 – 537	6	ATP 2 By similarity
	Nucleotide binding	747 – 748	2	ATP 2 By similarity
	Nucleotide binding	784 – 788	5	ATP 2 By similarity
	Nucleotide binding	863 – 867	5	ATP 2 By similarity
	Region	1 – 12	12	Hydrophobic
	Region	13 – 475	463	Regulatory
	Region	84 – 88	5	Glucose-6-phosphate 1 binding By similarity
	Region	172 – 173	2	Substrate 1 binding By similarity
	Region	208 – 209	2	Substrate 1 binding By similarity
	Region	291 – 294	4	Substrate 1 binding By similarity
	Region	413 – 415	3	Glucose-6-phosphate 1 binding By similarity
	Region	476 – 917	442	Catalytic
	Region	532 – 536	5	Glucose-6-phosphate 2 binding By similarity
	Region	603 – 604	2	Substrate 2 binding By similarity
	Region	620 – 621	2	Substrate 2 binding By similarity
	Region	656 – 657	2	Substrate 2 binding By similarity
	Region	861 – 863	3	Glucose-6-phosphate 2 binding By similarity
Sites
	Binding site	30	1	ATP 1 By similarity
	Binding site	155	1	Substrate 1 binding By similarity
	Binding site	209	1	Glucose-6-phosphate 1 By similarity
	Binding site	232	1	Glucose-6-phosphate 1 By similarity
	Binding site	235	1	Substrate 1 By similarity
	Binding site	260	1	Substrate 1 By similarity
	Binding site	449	1	Glucose-6-phosphate 1 By similarity
	Binding site	603	1	Glucose-6-phosphate 2 By similarity
	Binding site	657	1	Glucose-6-phosphate 2 By similarity
	Binding site	680	1	ATP 2 By similarity
	Binding site	680	1	Glucose-6-phosphate 2 By similarity
	Binding site	683	1	Substrate 2 binding By similarity
	Binding site	708	1	Substrate 2 By similarity
	Binding site	742	1	Substrate 2 By similarity
	Binding site	897	1	Glucose-6-phosphate 2 By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	27	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5RC71-1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: BCB4765425999C79	FASTA	917	102,363
	10 20 30 40 50 60 MIAAQLLAYY FTELKDDQVK KIDKYLYAMR LSDETLIDIM TRFRKEMKNG LSRDFNPTAT 70 80 90 100 110 120 VKMLPTFVRS IPDGSEKGDF IALDLGGSSF RILRVQVNHE KNQNVHMESE VYDTPENIVH 130 140 150 160 170 180 GSGSQLFDHV AECLGDFMEK RKIKDKKSPV GFTFSFPCQQ SKIDEAVLIT WTKRFKASGV 190 200 210 220 230 240 EGADVVKLLN KAIKKRGDYD ANIVAVVNDT VGTMMTCGYD DQHCEVGLII GTGTNACYME 250 260 270 280 290 300 ELRHIDLVEG DEGRMCINTE WGAFGDDGSL EDIRTEFDRE IDRGSLNPGK QLFEKMVSGM 310 320 330 340 350 360 YLGELVRLIL VKMAKEGLLF EGRITPELLT RGKFNTSDVS AIEKNKEGLH NAKEILTRLG 370 380 390 400 410 420 VEPSDDDCVS VQHVCTIVSF RSANLVAATL GAILNRLRDN KGTPRLRTTV GVDGSLYKTH 430 440 450 460 470 480 PQYSRRFHKT LRRLVPDSDV RFLLSESGSG KGAAMVTAVA YRLAEQHRQI EETLAHFHLT 490 500 510 520 530 540 KDMLLEVKKR MRAEMELGLR KQTHNNAAVK MLPSFVRRTP DGTENGDFLA LDLGGTNFRV 550 560 570 580 590 600 LLVKIRSGKK RTVEMHNKIY AIPIEIMQGT GEELFDHIVS CISDFLDYMG IKGPRMPLGF 610 620 630 640 650 660 TFSFPCKQTS LDAGILITWT KGFKATDCVG NDVATLLRDA IKRREEFDLD VVAVVNDTVG 670 680 690 700 710 720 TMMTCAYEEP TCEVGLIVGT GSNACYMEEM KNVEMVEGDQ GQMCINMEWG AFGDNGCLDD 730 740 750 760 770 780 IRTHYDRLVD EYSLNAGKQR YEKMISGMYL GEIVRNILID FTKKGFLFRG QISEPLKTRG 790 800 810 820 830 840 IFETKFLSQI ESDRLALLQV RAILQQLGLN STCDDSILVK TVCGVVSRRA AQLCGAGMAA 850 860 870 880 890 900 VVDKIRENRG LDRLNVTVGV DGTLYKLHPH FSRIMHQTVK ELSPKCNVSF LLSEDGSGKG 910 AALITAVGVR LRTEASS

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR858409 mRNA. Translation: CAH90636.1.
RefSeq	NP_001125344.1.
UniGene	Pab.19241
3D structure databases
SMR	Q5RC71. Positions 16-913.
ModBase	Search...
Genome annotation databases
GeneID	100172246.
Organism-specific databases
CTD	100172246.
Phylogenomic databases
HOVERGEN	Q5RC71.
Enzyme and pathway databases
BRENDA	2.7.1.1. 269192.
Family and domain databases
InterPro	IPR001312. Hexokinase. IPR019807. Hexokinase_CS. [Graphical view]
PANTHER	PTHR19443. Hexokinase. 1 hit.
Pfam	PF00349. Hexokinase_1. 2 hits. PF03727. Hexokinase_2. 2 hits. [Graphical view]
PRINTS	PR00475. HEXOKINASE.
ProDom	PD001109. Hexokinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00378. HEXOKINASES. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HXK1_PONAB

Accession

Primary (citable) accession number: Q5RC71

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	December 21, 2004
Last modified:	September 1, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)