Q5RC19 (ACOX1_PONAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peroxisomal acyl-coenzyme A oxidase 1
Short name=AOX
EC=1.3.3.6

Alternative name(s):
Palmitoyl-CoA oxidase

Gene names

Name:

ACOX1

Organism

Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) [Reference proteome]

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Ponginae › Pongo

Protein attributes

Sequence length	660 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs By similarity.
Catalytic activity	Acyl-CoA + O2 = trans-2,3-dehydroacyl-CoA + H2O2.
Cofactor	FAD By similarity.
Pathway	Lipid metabolism; peroxisomal fatty acid beta-oxidation.
Subcellular location	Peroxisome By similarity.
Sequence similarities	Belongs to the acyl-CoA oxidase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Peroxisome
Coding sequence diversity	Alternative splicing
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fatty acid beta-oxidation using acyl-CoA oxidase Inferred from electronic annotation. Source: UniProtKB-UniPathway fatty acid oxidation Inferred from sequence or structural similarity. Source: UniProtKB generation of precursor metabolites and energy Inferred from sequence or structural similarity. Source: UniProtKB prostaglandin metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	peroxisome Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	acyl-CoA dehydrogenase activity Inferred from electronic annotation. Source: InterPro acyl-CoA oxidase activity Inferred from sequence or structural similarity. Source: UniProtKB flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5RC19-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5RC19-2)

The sequence of this isoform differs from the canonical sequence as follows:
     90-131: KLHLVNFVEP...KWLLSSKGLQ → NFVHRGRPEP...RFFMPAWNLE

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 660	660	Peroxisomal acyl-coenzyme A oxidase 1		PRO_0000204680
Regions
	Motif	658 – 660	3	Microbody targeting signal By similarity
Sites
	Active site	421	1	Proton acceptor By similarity
	Binding site	139	1	FAD By similarity
	Binding site	178	1	FAD; via amide nitrogen By similarity
Amino acid modifications
	Modified residue	26	1	Phosphoserine By similarity
	Modified residue	255	1	N6-acetyllysine By similarity
	Modified residue	267	1	N6-acetyllysine By similarity
	Modified residue	310	1	Phosphoserine By similarity
	Modified residue	437	1	N6-acetyllysine By similarity
	Modified residue	500	1	N6-acetyllysine By similarity
	Modified residue	504	1	N6-acetyllysine By similarity
	Modified residue	643	1	N6-acetyllysine By similarity
	Modified residue	649	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	90 – 131	42	KLHLV…SKGLQ → NFVHRGRPEPLDLHLGMFLP TLLHQATAEQQERFFMPAWN LE in isoform 2.		VSP_013217
Experimental info
	Sequence conflict	284	1	F → L in CAH90691. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 29, 2005. Version 2. Checksum: 99A0A2EF19331211	FASTA	660	74,454
	10 20 30 40 50 60 MNPDLRRERD SASFNPELLT HILDGSPEKT RRRREIENMI LNDPDFQHED LNFLTRSQRY 70 80 90 100 110 120 EVAVRKSAIM VKKMREFGIA DPDEIMWFKK LHLVNFVEPV GLNYSMFIPT LLNQGTTAQK 130 140 150 160 170 180 EKWLLSSKGL QIIGTYAQTE MGHGTHLRGL ETTATYDPET QEFILNSPTV TSIKWWPGGL 190 200 210 220 230 240 GKTSNHAIVL AQLITKGKGY GLHAFIVPIR EIGTHKPLPG ITVGDIGPKF GYDEIDNGYL 250 260 270 280 290 300 KMDNYRIPRE NMLMKYAQVK PDGTYVKPLS NKLTYGTMVF VRSFLVGEAA RALSKACTIA 310 320 330 340 350 360 IRYSAVRHQS EIKPGEPEPQ ILDFQTQQYK LFPLLATAYA FQFVGAYMKE TYHRINEGIG 370 380 390 400 410 420 QGDLSELPEL HALTAGLKAF TSWTANTGIE ACRMACGGHG YSHCSGLPNI YVNFTPSCTF 430 440 450 460 470 480 EGENTVMMLQ TARFLMKSYD QVHSGKLVCG MVSYLNDLPS QRIQPQQVAV WPTMVDIHSP 490 500 510 520 530 540 ESLTEAYKLR AARLVEIAAK NLQKEVIHRK SKEVAWNLTS VDLVRASEAH CHYVVVKLFS 550 560 570 580 590 600 EKLLKIQDKA IQAVLRNLCL LYSLYGISQN AGDFLQGSIM TEPQITQVNQ RVKELLTLIR 610 620 630 640 650 660 SDAVALVDAF DFQDVTLGSV LGRYDGNVYE NLFEWAKNSP LNKAEVHESY KHLKSLQSKL
	Isoform 2 [UniParc]. Checksum: 38AF43D05A3BCCD8	FASTA	660	74,698
	10 20 30 40 50 60 MNPDLRRERD SASFNPELLT HILDGSPEKT RRRREIENMI LNDPDFQHED LNFLTRSQRY 70 80 90 100 110 120 EVAVRKSAIM VKKMREFGIA DPDEIMWFKN FVHRGRPEPL DLHLGMFLPT LLHQATAEQQ 130 140 150 160 170 180 ERFFMPAWNL EIIGTYAQTE MGHGTHLRGL ETTATYDPET QEFILNSPTV TSIKWWPGGL 190 200 210 220 230 240 GKTSNHAIVL AQLITKGKGY GLHAFIVPIR EIGTHKPLPG ITVGDIGPKF GYDEIDNGYL 250 260 270 280 290 300 KMDNYRIPRE NMLMKYAQVK PDGTYVKPLS NKLTYGTMVF VRSFLVGEAA RALSKACTIA 310 320 330 340 350 360 IRYSAVRHQS EIKPGEPEPQ ILDFQTQQYK LFPLLATAYA FQFVGAYMKE TYHRINEGIG 370 380 390 400 410 420 QGDLSELPEL HALTAGLKAF TSWTANTGIE ACRMACGGHG YSHCSGLPNI YVNFTPSCTF 430 440 450 460 470 480 EGENTVMMLQ TARFLMKSYD QVHSGKLVCG MVSYLNDLPS QRIQPQQVAV WPTMVDIHSP 490 500 510 520 530 540 ESLTEAYKLR AARLVEIAAK NLQKEVIHRK SKEVAWNLTS VDLVRASEAH CHYVVVKLFS 550 560 570 580 590 600 EKLLKIQDKA IQAVLRNLCL LYSLYGISQN AGDFLQGSIM TEPQITQVNQ RVKELLTLIR 610 620 630 640 650 660 SDAVALVDAF DFQDVTLGSV LGRYDGNVYE NLFEWAKNSP LNKAEVHESY KHLKSLQSKL

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Heart and Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR858463 mRNA. Translation: CAH90691.1. CR859802 mRNA. Translation: CAH91960.1.
RefSeq	NP_001125380.1. NM_001131908.1. NP_001128843.1. NM_001135371.1.
UniGene	Pab.19225. Pab.19725.
3D structure databases
ProteinModelPortal	Q5RC19.
SMR	Q5RC19. Positions 1-654.
ModBase	Search...
Proteomic databases
PRIDE	Q5RC19.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100172283. 100189759.
KEGG	pon:100172283. pon:100189759.
Organism-specific databases
CTD	51.
Phylogenomic databases
HOVERGEN	HBG050451.
KO	K00232.
Enzyme and pathway databases
UniPathway	UPA00661.
Family and domain databases
Gene3D	1.10.540.10. 1 hit. 2.40.110.10. 1 hit.
InterPro	IPR006091. Acyl-CoA_Oxase/DH_cen-dom. IPR012258. Acyl-CoA_oxidase. IPR002655. Acyl-CoA_oxidase_C. IPR009075. AcylCo_DH/oxidase_C. IPR013786. AcylCoA_DH/ox_N. IPR009100. AcylCoA_DH/oxidase. [Graphical view]
PANTHER	PTHR10909:SF11. PTHR10909:SF11. 1 hit.
Pfam	PF01756. ACOX. 1 hit. PF02770. Acyl-CoA_dh_M. 1 hit. [Graphical view]
PIRSF	PIRSF000168. Acyl-CoA_oxidase. 1 hit.
SUPFAM	SSF56645. AcylCoA_dehyd_NM. 1 hit. SSF47203. AcylCoADH_C_like. 2 hits.
ProtoNet	Search...

Entry information

Entry name

ACOX1_PONAB

Accession

Primary (citable) accession number: Q5RC19
Secondary accession number(s): Q5R8F0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	March 29, 2005
Last modified:	March 6, 2013
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program