Q5RBJ3 (TPMT_PONPY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiopurine S-methyltransferase
EC=2.1.1.67

Alternative name(s):
Thiopurine methyltransferase

Gene names

Name:

TPMT

Organism

Pongo pygmaeus (Bornean orangutan)

Taxonomic identifier

9600 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine.
Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Thiopurine S-methyltransferase		PRO_0000220111
Sites
	Binding site	33	1	S-adenosyl-L-methionine By similarity
	Binding site	69	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	90	1	S-adenosyl-L-methionine By similarity
	Binding site	152	1	S-adenosyl-L-methionine By similarity
Amino acid modifications
	Modified residue	14	1	Phosphoserine By similarity
	Modified residue	58	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	112	1	V → I in AAX37647. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q5RBJ3 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 47D893B0E1E0A01C	FASTA	245	28,242
	10 20 30 40 50 60 MDGIRTSLDI EEYSDTEVQK NQVLTLEEWQ DKWVNGNTAF HQEQGHRLLK KHLDTFLKGE 70 80 90 100 110 120 SGLRVFFPLC GKAVEMKWFA DRGHSVVGVE ISELGIREFF TEQNLSYSEE PVTEIPGTKI 130 140 150 160 170 180 FKSSSGNISL YCCSIFDLPR TNIGKFDMIW DRGALVAINP GDRKCYADTM LSLLGKKFQY 190 200 210 220 230 240 LLCVLSYDPT KHPGPPFYVP HAEIERLFGK ICNIHCLEKV DAFEERHKSW GIDYLFEKLY LLTEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Thiopurine S-methyltransferase pharmacogenetics: variant allele functional and comparative genomics." Salavaggione O.E., Wang L., Wiepert M., Yee V.C., Weinshilboum R.M. Pharmacogenet. Genomics 15:801-815(2005) [PubMed: 16220112] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY827083 mRNA. Translation: AAX37647.1. CR858654 mRNA. Translation: CAH90867.1.
UniGene	Pab.19187.
3D structure databases
ProteinModelPortal	Q5RBJ3.
SMR	Q5RBJ3. Positions 17-245.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5RBJ3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
GeneTree	ENSGT00390000016823.
HOVERGEN	HBG003037.
InParanoid	Q5RBJ3.
OrthoDB	EOG4C5CKF.
Family and domain databases
InterPro	IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
PANTHER	PTHR10259. PTHR10259. 1 hit.
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_PONPY

Accession

Primary (citable) accession number: Q5RBJ3
Secondary accession number(s): Q3BCQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	December 21, 2004
Last modified:	October 19, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program