Reviewed, UniProtKB/Swiss-Prot Q5RBI4 (ABHD5_PONAB)

Names and origin

Protein names

Recommended name:
    1-acylglycerol-3-phosphate O-acyltransferase ABHD5
    EC=2.3.1.51
Alternative name(s):
    Abhydrolase domain-containing protein 5

Gene names

Name:

ABHD5

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation By similarity.
Catalytic activity	Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.
Subunit structure	Interacts with ADRP, PLIN and PNPLA2 By similarity.
Subcellular location	Cytoplasm By similarity. Lipid droplet By similarity. Note: Colocalized with PLIN and ADRP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.
Sequence similarities	Belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily.

Ontologies

Keywords
Biological process	Differentiation Fatty acid metabolism Lipid metabolism Phospholipid biosynthesis
Cellular component	Cytoplasm Lipid droplet
Disease	Cataract Deafness Disease mutation Ichthyosis
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of sequestering of triglyceride Inferred from sequence or structural similarity. Source: UniProtKB phosphatidic acid biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of triglyceride catabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB monolayer-surrounded lipid storage body Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-acylglycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	1-acylglycerol-3-phosphate O-acyltransferase ABHD5		PRO_0000080869
Regions
	Motif	327 – 332	6	HXXXXD motif

Sequences

	Sequence		Length	Mass (Da)
	Q5RBI4-1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 49A6A8EC49A6023B	FASTA	349	39,065
	10 20 30 40 50 60 MAAEEEEVDS ADTGERSGWL TGWLPTWCPT STSHLKEAEE KMLKCVPCTY KKEPVHISNG 70 80 90 100 110 120 NKIWTLKFSH NISNKTPLVL LHGFGGGLGL WALNFGDLCT NRPVYAFDLL GFGRSSRPRF 130 140 150 160 170 180 DSDAEEVENQ FVESIEEWRC ALGLDKMILL GHNLGGFLAA AYSLKYPSRV NHLILVEPWG 190 200 210 220 230 240 FPERPDLADQ DRPIPVWIRA LGAALTPFNP LAGLRIAGPF GLSLVQRLRP DFKRKYSSMF 250 260 270 280 290 300 EDDTVTEYIY HCNVQTPSGE TAFKNMTIPY GWAKRPMLQR IGKMHPDIPV SVIFGARSCI 310 320 330 340 DGNSGTSIQS LRPHSYVKTI AILGAGHYVY ADQPEEFNQK VKEICDTVD

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR858663 mRNA. Translation: CAH90876.1.
RefSeq	NP_001127344.1.
UniGene	Pab.19184
3D structure databases
SMR	Q5RBI4. Positions 12-177, 45-347.
ModBase	Search...
Genome annotation databases
GeneID	100174407.
Organism-specific databases
CTD	100174407.
Phylogenomic databases
HOVERGEN	Q5RBI4.
InParanoid	Q5RBI4.
OMA	LNFGDLC.
Family and domain databases
InterPro	IPR000073. AB_hydrolase_1. [Graphical view]
Pfam	PF00561. Abhydrolase_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ABHD5_PONAB

Accession

Primary (citable) accession number: Q5RBI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	December 21, 2004
Last modified:	February 9, 2010
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)