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Q5RBG4

- KAT5_PONAB

UniProt

Q5RBG4 - KAT5_PONAB

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Protein
Histone acetyltransferase KAT5
Gene
KAT5, HTATIP, TIP60
Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. Relieves NR1D2-mediated inhibition of APOC3 expression by acetylating NR1D2. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome By similarity.

Catalytic activityi

Acetyl-CoA + [histone] = CoA + acetyl-[histone].

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei275 – 2751 By similarity
Active sitei317 – 3171Nucleophile By similarity
Binding sitei320 – 3201Acetyl-CoA By similarity
Binding sitei355 – 3551Acetyl-CoA By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri209 – 23123C2HC-type
Add
BLAST

GO - Molecular functioni

  1. histone acetyltransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. transcription coactivator activity Source: UniProtKB

GO - Biological processi

  1. DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator Source: UniProtKB
  2. histone acetylation Source: GOC
  3. positive regulation of transcription, DNA-templated Source: UniProtKB
  4. regulation of growth Source: UniProtKB-KW
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Acyltransferase, Chromatin regulator, Transferase

Keywords - Biological processi

Growth regulation, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Histone acetyltransferase KAT5 (EC:2.3.1.48)
Alternative name(s):
60 kDa Tat-interactive protein
Short name:
Tip60
Histone acetyltransferase HTATIP
Lysine acetyltransferase 5
Gene namesi
Name:KAT5
Synonyms:HTATIP, TIP60
OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Taxonomic identifieri9601 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
ProteomesiUP000001595: Unplaced

Subcellular locationi

Nucleus By similarity. Nucleusnucleolus By similarity. Cytoplasmperinuclear region By similarity
Note: Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region By similarity.

GO - Cellular componenti

  1. NuA4 histone acetyltransferase complex Source: UniProtKB
  2. Piccolo NuA4 histone acetyltransferase complex Source: UniProtKB
  3. Swr1 complex Source: UniProtKB
  4. nucleolus Source: UniProtKB
  5. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 461461Histone acetyltransferase KAT5
PRO_0000245806Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei52 – 521N6-acetyllysine By similarity
Modified residuei86 – 861Phosphoserine By similarity
Modified residuei90 – 901Phosphoserine By similarity
Modified residuei275 – 2751N6-acetyllysine; by autocatalysis By similarity
Cross-linki378 – 378Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Cross-linki399 – 399Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Post-translational modificationi

Sumoylated by UBE2I at Lys-378 and Lys-399, leading to increase of its histone acetyltransferase activity in UV-induced DNA damage response, as well as its translocation to nuclear bodies By similarity.
Phosphorylated on Ser-86 and Ser-90; enhanced during G2/M phase. Phosphorylated form has a higher activity By similarity.
Ubiquitinated by MDM2, leading to its proteasome-dependent degradation By similarity.
Autoacetylation at Lys-275 is required for proper function By similarity.

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ5RBG4.

Interactioni

Subunit structurei

Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6 By similarity. HTATTIP/TIP60, EPC1, and ING3 together constitute a minimal HAT complex termed Piccolo NuA4 By similarity. The NuA4 complex interacts with MYC By similarity. Interacts with ATM By similarity. Interacts with JADE1 By similarity. Interacts with PLA2G4A/CPLA2, EDNRA and HDAC7 By similarity. Interacts with the cytoplasmic tail of APP. Interacts with the cytoplasmic tail of APP and APBB1/FE65 By similarity. Interacts with TRIM24 and TRIM68 By similarity. Forms a complex with SENP6 and UBE2I in response to UV irradiation. Identified in a complex with HINT1 By similarity. Interacts with ATF2 and CUL3. Interacts with NR1D2 (via N-terminus). Component of a SWR1-like complex By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ5RBG4.
SMRiQ5RBG4. Positions 11-79, 178-452.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni316 – 461146Interaction with ATF2 By similarity
Add
BLAST
Regioni325 – 3317Acetyl-CoA binding By similarity

Sequence similaritiesi

Belongs to the MYST (SAS/MOZ) family.

Keywords - Domaini

Zinc-finger

Phylogenomic databases

KOiK11304.

Family and domain databases

Gene3Di3.40.630.30. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR000953. Chromo_domain/shadow.
IPR016197. Chromodomain-like.
IPR002717. MOZ_SAS.
IPR025995. Tudor-knot.
[Graphical view]
PfamiPF01853. MOZ_SAS. 1 hit.
PF11717. Tudor-knot. 1 hit.
[Graphical view]
SMARTiSM00298. CHROMO. 1 hit.
[Graphical view]
SUPFAMiSSF54160. SSF54160. 1 hit.
SSF55729. SSF55729. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5RBG4-1 [UniParc]FASTAAdd to Basket

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MAEVGEIIEG CRLPVLRRNQ DNEDEWPLAE ILSVKDISGR KLFYVHYIDF    50
NKRLDEWVTH ERLDLKKIQF PKKEAKTPTK NGLPGSRPGS PEREVKRKVE 100
VVSPATPVPS ETAPASVFPQ NGAARRAVAA QPGRKRKSNC LGTDEDSQDS 150
SDGIPSAPRM TGSLVSDRSH DDIVTRMKNI ECIELGRHRL KPWYFSPYPQ 200
ELTTLPVLYL CEFCLKYGRS LKCLQRHLTK CDLRHPPGNE IYRKGTISFF 250
EIDGRKNKSY SQNLCLLAKC FLDHKTLYYD TDPFLFYVMT EYDCKGFHIV 300
GYFSKEKEST EDYNVACILT LPPYQRRGYG KLLIEFSYEL SKVEGKTGTP 350
EKPLSDLGLL SYRSYWSQTI LEILMGLKSE SGERPQITIN EISEITSIKK 400
EDVISTLQYL NLINYYKGQY ILTLSEDIVD GHERAMLKRL LRIDSKCLHF 450
TPKDWSKRGK W 461
Length:461
Mass (Da):53,077
Last modified:December 21, 2004 - v1
Checksum:i5A0E9324550AF246
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR858684 mRNA. Translation: CAH90896.1.
RefSeqiNP_001127347.1. NM_001133875.1.
UniGeneiPab.13524.

Genome annotation databases

GeneIDi100174410.
KEGGipon:100174410.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR858684 mRNA. Translation: CAH90896.1 .
RefSeqi NP_001127347.1. NM_001133875.1.
UniGenei Pab.13524.

3D structure databases

ProteinModelPortali Q5RBG4.
SMRi Q5RBG4. Positions 11-79, 178-452.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q5RBG4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100174410.
KEGGi pon:100174410.

Organism-specific databases

CTDi 10524.

Phylogenomic databases

KOi K11304.

Family and domain databases

Gene3Di 3.40.630.30. 1 hit.
InterProi IPR016181. Acyl_CoA_acyltransferase.
IPR000953. Chromo_domain/shadow.
IPR016197. Chromodomain-like.
IPR002717. MOZ_SAS.
IPR025995. Tudor-knot.
[Graphical view ]
Pfami PF01853. MOZ_SAS. 1 hit.
PF11717. Tudor-knot. 1 hit.
[Graphical view ]
SMARTi SM00298. CHROMO. 1 hit.
[Graphical view ]
SUPFAMi SSF54160. SSF54160. 1 hit.
SSF55729. SSF55729. 1 hit.
ProtoNeti Search...

Publicationsi

  1. The German cDNA consortium
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.

Entry informationi

Entry nameiKAT5_PONAB
AccessioniPrimary (citable) accession number: Q5RBG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: December 21, 2004
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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