Q5RBG4 (KAT5_PONAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histone acetyltransferase KAT5
EC=2.3.1.48

Alternative name(s):
60 kDa Tat-interactive protein
Short name=Tip60
Histone acetyltransferase HTATIP
Lysine acetyltransferase 5

Gene names

Name:	KAT5
Synonyms:	HTATIP, TIP60

Organism

Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) [Reference proteome]

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Ponginae › Pongo

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM By similarity.
Catalytic activity	Acetyl-CoA + [histone] = CoA + acetyl-[histone].
Subunit structure	Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6 By similarity. HTATTIP/TIP60, EPC1, and ING3 together constitute a minimal HAT complex termed Piccolo NuA4 By similarity. The NuA4 complex interacts with MYC By similarity. Interacts with ATM By similarity. Interacts with PHF17 By similarity. Interacts with PLA2G4A/CPLA2, EDNRA and HDAC7 By similarity. Interacts with the cytoplasmic tail of APP. Interacts with the cytoplasmic tail of APP and APBB1/FE65 By similarity. Interacts with TRIM24 and TRIM68 By similarity. Forms a complex with SENP6 and UBE2I in response to UV irradiation. Identified in a complex with HINT1 By similarity.
Subcellular location	Nucleus › nucleolus By similarity. Cytoplasm › perinuclear region By similarity. Note: Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region By similarity.
Post-translational modification	Sumoylated by UBE2I at Lys-378 and Lys-399, leading to increase of its histone acetyltransferase activity in UV-induced DNA damage response, as well as its translocation to nuclear bodies By similarity. Phosphorylated on Ser-86 and Ser-90; enhanced during G2/M phase. Phosphorylated form has a higher activity By similarity. Ubiquitinated by MDM2, leading to its proteasome-dependent degradation By similarity. Autoacetylation at Lys-275 is required for proper function By similarity.
Sequence similarities	Belongs to the MYST (SAS/MOZ) family. Contains 1 C2HC-type zinc finger.

Ontologies

Keywords
Biological process	Growth regulation Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Activator Acyltransferase Chromatin regulator Transferase
PTM	Acetylation Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator Inferred from sequence or structural similarity. Source: UniProtKB histone acetylation Inferred from sequence or structural similarity. Source: GOC positive regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB regulation of growth Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Piccolo NuA4 histone acetyltransferase complex Inferred from sequence or structural similarity. Source: UniProtKB nucleolus Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	histone acetyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW transcription coactivator activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Histone acetyltransferase KAT5		PRO_0000245806
Regions
	Zinc finger	209 – 231	23	C2HC-type
	Region	325 – 331	7	Acetyl-CoA binding By similarity
Sites
	Active site	275	1	By similarity
	Active site	317	1	Nucleophile By similarity
	Binding site	320	1	Acetyl-CoA By similarity
	Binding site	355	1	Acetyl-CoA By similarity
Amino acid modifications
	Modified residue	52	1	N6-acetyllysine By similarity
	Modified residue	86	1	Phosphoserine By similarity
	Modified residue	90	1	Phosphoserine By similarity
	Modified residue	275	1	N6-acetyllysine; by autocatalysis By similarity
	Cross-link	378		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
	Cross-link	399		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5RBG4 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 5A0E9324550AF246	FASTA	461	53,077
	10 20 30 40 50 60 MAEVGEIIEG CRLPVLRRNQ DNEDEWPLAE ILSVKDISGR KLFYVHYIDF NKRLDEWVTH 70 80 90 100 110 120 ERLDLKKIQF PKKEAKTPTK NGLPGSRPGS PEREVKRKVE VVSPATPVPS ETAPASVFPQ 130 140 150 160 170 180 NGAARRAVAA QPGRKRKSNC LGTDEDSQDS SDGIPSAPRM TGSLVSDRSH DDIVTRMKNI 190 200 210 220 230 240 ECIELGRHRL KPWYFSPYPQ ELTTLPVLYL CEFCLKYGRS LKCLQRHLTK CDLRHPPGNE 250 260 270 280 290 300 IYRKGTISFF EIDGRKNKSY SQNLCLLAKC FLDHKTLYYD TDPFLFYVMT EYDCKGFHIV 310 320 330 340 350 360 GYFSKEKEST EDYNVACILT LPPYQRRGYG KLLIEFSYEL SKVEGKTGTP EKPLSDLGLL 370 380 390 400 410 420 SYRSYWSQTI LEILMGLKSE SGERPQITIN EISEITSIKK EDVISTLQYL NLINYYKGQY 430 440 450 460 ILTLSEDIVD GHERAMLKRL LRIDSKCLHF TPKDWSKRGK W

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR858684 mRNA. Translation: CAH90896.1.
RefSeq	NP_001127347.1. NM_001133875.1.
UniGene	Pab.13524.
3D structure databases
ProteinModelPortal	Q5RBG4.
SMR	Q5RBG4. Positions 11-79, 178-452.
ModBase	Search...
Proteomic databases
PRIDE	Q5RBG4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100174410.
KEGG	pon:100174410.
Organism-specific databases
CTD	10524.
Phylogenomic databases
KO	K11304.
Family and domain databases
Gene3D	3.40.630.30. 1 hit.
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000953. Chromo_domain/shadow. IPR016197. Chromodomain-like. IPR002717. MOZ_SAS. IPR025995. Tudor-knot. [Graphical view]
Pfam	PF01853. MOZ_SAS. 1 hit. PF11717. Tudor-knot. 1 hit. [Graphical view]
SMART	SM00298. CHROMO. 1 hit. [Graphical view]
SUPFAM	SSF55729. Acyl_CoA_acyltransferase. 1 hit. SSF54160. Chromodomain-like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KAT5_PONAB

Accession

Primary (citable) accession number: Q5RBG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	December 21, 2004
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program