Reviewed, UniProtKB/Swiss-Prot Q5RBE8 (LOX15_PONAB)

Names and origin

Protein names

Recommended name:
    Arachidonate 15-lipoxygenase
      Short name=15-LOX
    EC=1.13.11.33
Alternative name(s):
    Arachidonate omega-6 lipoxygenase

Gene names

Name:

ALOX15

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	662 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid By similarity.
Catalytic activity	Arachidonate + O2 = (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate.
Cofactor	Binds 1 iron ion per subunit By similarity.
Pathway	Lipid metabolism; leukotriene D4 biosynthesis.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.

Ontologies

Keywords
Biological process	Leukotriene biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	leukotriene biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	arachidonate 15-lipoxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW lipoxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 662	661	Arachidonate 15-lipoxygenase		PRO_0000220698
Regions
	Domain	2 – 114	113	PLAT
	Domain	115 – 662	548	Lipoxygenase
Sites
	Metal binding	360	1	Iron; catalytic By similarity
	Metal binding	365	1	Iron; catalytic By similarity
	Metal binding	540	1	Iron; catalytic By similarity
	Metal binding	662	1	Iron; via carboxylate; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5RBE8-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 375A826C56D9EEE6	FASTA	662	74,633
	10 20 30 40 50 60 MGLYRIRVST GASLYAGSNN QVQLWLVGQH GEAALGTRLW PARGKETELK VEVPEYLGPL 70 80 90 100 110 120 LFVKLRKRHL LTDDAWFCNW ISVQGPGAGD EVRFPCYRWV EGNGVLSLPE GTGRTVGDDP 130 140 150 160 170 180 QGLFQKHREE ELEERRKLYR WGNWKDGLIL NVAGAKLCDL PVDERFLEDK RVDFEVSLAK 190 200 210 220 230 240 GLADLAIKDS LNVLTCWKDL DDFNRIFWCG QSKLAERVRD SWKEDALFGY QFLNGANPVV 250 260 270 280 290 300 LRRSVHLPAR LVFPPGMEEL QAQLEKELEG GTLFEADFSL LDGIKANVIL CSQQHLAAPL 310 320 330 340 350 360 VMLKLQPDGK LLPMVIQLQL PSTGSPPPPL FLPTDPPMAW LLAKCWVRSS DFQLHELQSH 370 380 390 400 410 420 LLRGHLMAEV IVVATMRCLP SIHPIFKLII PHLRYTLEIN VRARTGLVSD MGIFDQIMST 430 440 450 460 470 480 GGGGHVQLLK QAGAFLTYSS FCPPDDLADR GLLGVKSSFY AQDALRLWEI IYRYVEGIVS 490 500 510 520 530 540 LHYKTDVAVK DDPELQTWCR EITEIGLQGA QDRGFPVSLQ SRDQVCHFVT MCIFTCTGQH 550 560 570 580 590 600 ASVHLGQLDW YSWVPNAPCT MRLPPPTTKD ATLETVMATL PNFHQASLQM SITWQLGRRQ 610 620 630 640 650 660 PVMVAVGQHE EEYFSGPEPK AVLKKFREEL AALDKEIEIR NAKLDMPYEY LRPSIVENSV AI

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR858703 mRNA. Translation: CAH90912.1.
RefSeq	NP_001127349.1.
UniGene	Pab.13552
3D structure databases
SMR	Q5RBE8. Positions 1-661, 2-662.
ModBase	Search...
Genome annotation databases
GeneID	100174413.
Phylogenomic databases
HOVERGEN	Q5RBE8.
OMA	Q5RBE8. WQLGRRQ.
Enzyme and pathway databases
BRENDA	1.13.11.33. 269192.
Family and domain databases
InterPro	IPR000907. LipOase. IPR013819. LipOase_C. IPR001024. LipOase_LH2. IPR001885. LipOase_mml. [Graphical view]
Gene3D	G3DSA:2.60.60.20. Lipase_LipOase. 1 hit.
PANTHER	PTHR11771. LipOase. 1 hit.
Pfam	PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PRINTS	PR00087. LIPOXYGENASE. PR00467. MAMLPOXGNASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
PROSITE	PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LOX15_PONAB

Accession

Primary (citable) accession number: Q5RBE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 37 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)