Q5RBC3 (EXT1_PONAB) Reviewed, UniProtKB/Swiss-Prot
Last modified
October 3, 2012.
Version 41.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Exostosin-1 EC=2.4.1.224 EC=2.4.1.225 Alternative name(s): Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase Multiple exostoses protein 1 homolog | ||
| Gene names |
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| Organism | Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) [Reference proteome] | ||
| Taxonomic identifier | 9601 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo |
Protein attributes
| Sequence length | 746 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Glycosyltransferase required for the biosynthesis of heparan-sulfate. The EXT1/EXT2 complex possesses substantially higher glycosyltransferase activity than EXT1 or EXT2 alone By similarity. |
| Catalytic activity | UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan = UDP + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-proteoglycan. UDP-alpha-D-glucuronate + N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan = UDP + beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan. |
| Pathway | |
| Subunit structure | Forms a homo/hetero-oligomeric complex with EXT2 By similarity. |
| Subcellular location | Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Note: The EXT1/EXT2 complex is localized in the Golgi apparatus By similarity. |
| Sequence similarities | Belongs to the glycosyltransferase 47 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Endoplasmic reticulum Golgi apparatus Membrane |
| Domain | Signal-anchor Transmembrane Transmembrane helix |
| Molecular function | Glycosyltransferase Transferase |
| PTM | Glycoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway |
| Cellular_component | Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membraneInferred from electronic annotation. Source: UniProtKB-KW intrinsic to endoplasmic reticulum membraneInferred from electronic annotation. Source: InterPro |
| Molecular_function | N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity Inferred from electronic annotation. Source: EC glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 746 | 746 | Exostosin-1 | PRO_0000366931 | |||||
Regions | |||||||||
| Topological domain | 1 – 7 | 7 | Cytoplasmic Potential | ||||||
| Transmembrane | 8 – 28 | 21 | Helical; Signal-anchor for type II membrane protein; Potential | ||||||
| Topological domain | 29 – 746 | 718 | Lumenal Potential | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 89 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 330 | 1 | N-linked (GlcNAc...) Potential | ||||||
Sequences
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References
| [1] | The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | CR858728 mRNA. Translation: CAH90937.1. |
| RefSeq | NP_001125538.1. NM_001132066.1. |
| UniGene | Pab.9186. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1OMX based on UniProtKB Q9ES89. |
| ProteinModelPortal | Q5RBC3. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | GT47. Glycosyltransferase Family 47. GT64. Glycosyltransferase Family 64. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 100172450. |
| KEGG | pon:100172450. |
Organism-specific databases | |
| CTD | 2131. |
Phylogenomic databases | |
| HOGENOM | HOG000266990. |
| HOVERGEN | HBG003459. |
| InParanoid | Q5RBC3. |
| KO | K02366. |
Enzyme and pathway databases | |
| UniPathway | UPA00378. |
Family and domain databases | |
| InterPro | IPR004263. Exostosin. IPR015338. HexNAc_Trfase_a. [Graphical view] |
| Pfam | PF03016. Exostosin. 1 hit. PF09258. Glyco_transf_64. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | EXT1_PONAB | ||||||||
| Accession | Primary (citable) accession number: Q5RBC3 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |