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Protein

Ectonucleotide pyrophosphatase/phosphodiesterase family member 6

Gene

ENPP6

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Choline-specific glycerophosphodiester phosphodiesterase. The preferred substrate may be lysosphingomyelin. Hydrolyzes lysophosphatidylcholine (LPC) to form monoacylglycerol and phosphorylcholine but not lysophosphatidic acid, showing it has a lysophospholipase C activity. Has a preference for LPC with short (12:0 and 14:0) or polyunsaturated (18:2 and 20:4) fatty acids. Also hydrolyzes glycerophosphorylcholine and sphingosylphosphorylcholine efficiently. Hydrolyzes the classical substrate for phospholipase C, p-nitrophenyl phosphorylcholine in vitro, while it does not hydrolyze the classical nucleotide phosphodiesterase substrate, p-nitrophenyl thymidine 5'-monophosphate. Does not hydrolyze diacyl phospholipids such as phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylglycerol and phosphatidic acid (By similarity).By similarity

Catalytic activityi

A lysophosphatidylcholine + H2O = a monoacylglycerol + phosphocholine.
sn-glycero-3-phosphocholine + H2O = glycerol + phosphocholine.

Cofactori

Enzyme regulationi

Inhibited by EDTA and EGTA in vitro.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei71NucleophileSequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Ectonucleotide pyrophosphatase/phosphodiesterase family member 6 (EC:3.1.4.-, EC:3.1.4.38)
Short name:
E-NPP 6
Short name:
NPP-6
Alternative name(s):
Choline-specific glycerophosphodiester phosphodiesterase
Glycerophosphocholine cholinephosphodiesterase
Short name:
GPC-Cpde
Gene namesi
Name:ENPP6
OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Taxonomic identifieri9601 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
Proteomesi
  • UP000001595 Componenti: Chromosome 4

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22By similarityAdd BLAST22
ChainiPRO_000036692323 – 418Ectonucleotide pyrophosphatase/phosphodiesterase family member 6Add BLAST396
PropeptideiPRO_0000420893419 – 440Removed in mature formSequence analysisAdd BLAST22

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei71PhosphoserineBy similarity1
Glycosylationi100N-linked (GlcNAc...)By similarity1
Glycosylationi118N-linked (GlcNAc...)By similarity1
Disulfide bondi142 ↔ 154By similarity
Glycosylationi341N-linked (GlcNAc...)By similarity1
Glycosylationi404N-linked (GlcNAc...)By similarity1
Disulfide bondi412InterchainBy similarity
Lipidationi418GPI-anchor amidated alanineSequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Interactioni

Subunit structurei

Homodimer; disulfide-linked.By similarity

Protein-protein interaction databases

STRINGi9601.ENSPPYP00000017018.

Structurei

3D structure databases

ProteinModelPortaliQ5RB45.
SMRiQ5RB45.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2645. Eukaryota.
COG1524. LUCA.
GeneTreeiENSGT00760000119157.
HOGENOMiHOG000112376.
HOVERGENiHBG107851.
InParanoidiQ5RB45.
KOiK08743.
OMAiCNVVGIT.
OrthoDBiEOG091G06NA.
TreeFamiTF330032.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024873. E-NPP.
IPR029889. ENPP6.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PANTHERiPTHR10151. PTHR10151. 1 hit.
PTHR10151:SF66. PTHR10151:SF66. 1 hit.
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5RB45-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVKLGTLLL ALALGLAQPA SARRKLLVFL LDGFRSDYIS DEALESLPGF
60 70 80 90 100
KEIVSRGVKV DYLTPDFPSL SYPNYYTLMT GRHCEVHQMI GNYMWDPTTN
110 120 130 140 150
KSFDIGVNKD SLMPLWWNGS EPLWVTLTKA KRKVYMYYWP GCEVEILGVR
160 170 180 190 200
PTYCLEYKNV PTDINFANAV SDALDSFKSG RADLAAIYHE RIDVEGHHYG
210 220 230 240 250
PASPQRKDAL KAVDTVLKYM TKWIQERGLQ DRLNVIIFSD HGMTDIFWMD
260 270 280 290 300
KVIELNKYIS LNDLQQAKDR GPVVSLWPAP GKHSEIYNKL STVEHMTVYE
310 320 330 340 350
KEAIPSRFYY KKGKFVSPLT LVADEGWFIT ENREMLPFWM NSTGRGNGWQ
360 370 380 390 400
RGWHGYDNEL MDMRGIFLAF GPDFKSNFRA APIRSVDVYN VMCNVVGITP
410 420 430 440
LPNNGSWSRV MCMLKGRAGT TPPVQPSHCA LALILLFLLA
Length:440
Mass (Da):50,041
Last modified:February 6, 2013 - v2
Checksum:i04D2D598E1829E40
GO
Isoform 2 (identifier: Q5RB45-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     373-440: DFKSNFRAAP...LALILLFLLA → AWIISALSPK...LECSGVMAYL

Note: No experimental confirmation available.
Show »
Length:435
Mass (Da):49,539
Checksum:i12884F6C365686A5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti332N → S in CAH91015 (Ref. 1) Curated1
Sequence conflicti346 – 347GN → RE in CAH91015 (Ref. 1) Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_044950373 – 440DFKSN…LFLLA → AWIISALSPKHYPCGAGGHE LDGMGKKSQFSFLLKCTEFD FIFPIDGLSLSSRLECSGVM AYL in isoform 2. 1 PublicationAdd BLAST68

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR858810 mRNA. Translation: CAH91015.1.
RefSeqiNP_001127363.1. NM_001133891.1.
UniGeneiPab.12335.

Genome annotation databases

EnsembliENSPPYT00000017708; ENSPPYP00000017018; ENSPPYG00000015233. [Q5RB45-1]
GeneIDi100174428.
KEGGipon:100174428.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR858810 mRNA. Translation: CAH91015.1.
RefSeqiNP_001127363.1. NM_001133891.1.
UniGeneiPab.12335.

3D structure databases

ProteinModelPortaliQ5RB45.
SMRiQ5RB45.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9601.ENSPPYP00000017018.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSPPYT00000017708; ENSPPYP00000017018; ENSPPYG00000015233. [Q5RB45-1]
GeneIDi100174428.
KEGGipon:100174428.

Organism-specific databases

CTDi133121.

Phylogenomic databases

eggNOGiKOG2645. Eukaryota.
COG1524. LUCA.
GeneTreeiENSGT00760000119157.
HOGENOMiHOG000112376.
HOVERGENiHBG107851.
InParanoidiQ5RB45.
KOiK08743.
OMAiCNVVGIT.
OrthoDBiEOG091G06NA.
TreeFamiTF330032.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR017850. Alkaline_phosphatase_core.
IPR024873. E-NPP.
IPR029889. ENPP6.
IPR002591. Phosphodiest/P_Trfase.
[Graphical view]
PANTHERiPTHR10151. PTHR10151. 1 hit.
PTHR10151:SF66. PTHR10151:SF66. 1 hit.
PfamiPF01663. Phosphodiest. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiENPP6_PONAB
AccessioniPrimary (citable) accession number: Q5RB45
Secondary accession number(s): H2PEU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: February 6, 2013
Last modified: November 2, 2016
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.