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Q5RAY1

- P3C2A_PONAB

UniProt

Q5RAY1 - P3C2A_PONAB

Protein

Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha

Gene

PIK3C2A

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (21 Dec 2004)
      Previous versions | rss
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    Functioni

    Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. Has a role in several intracellular trafficking events. Functions in insulin signaling and secretion. Required for translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane and glucose uptake in response to insulin-mediated RHOQ activation. Regulates insulin secretion through two different mechanisms: involved in glucose-induced insulin secretion downstream of insulin receptor in a pathway that involves AKT1 activation and TBC1D4/AS160 phosphorylation, and participates in the late step of insulin granule exocytosis probably in insulin granule fusion. Synthesizes PtdIns3P in response to insulin signaling. Functions in clathrin-coated endocytic vesicle formation and distribution. Regulates dynamin-independent endocytosis, probably by recruiting EEA1 to internalizing vesicles. In neurosecretory cells synthesizes PtdIns3P on large dense core vesicles. Participates in calcium induced contraction of vascular smooth muscle by regulating myosin light chain (MLC) phosphorylation through a mechanism involving Rho kinase-dependent phosphorylation of the MLCP-regulatory subunit MYPT1. May play a role in the EGF signaling cascade. May be involved in mitosis and UV-induced damage response. Required for maintenance of normal renal structure and function by supporting normal podocyte function By similarity.By similarity

    Catalytic activityi

    ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate.

    Cofactori

    Calcium or magnesium. Manganese cannot be used By similarity.By similarity

    Enzyme regulationi

    Only slightly inhibited by wortmannin and LY294002. Activated by clathrin and insulin By similarity.By similarity

    GO - Molecular functioni

    1. 1-phosphatidylinositol-4-phosphate 3-kinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW
    3. phosphatidylinositol binding Source: InterPro

    GO - Biological processi

    1. endocytosis Source: UniProtKB
    2. exocytosis Source: UniProtKB-KW
    3. phosphatidylinositol-mediated signaling Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Endocytosis, Exocytosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha (EC:2.7.1.154)
    Short name:
    PI3K-C2-alpha
    Short name:
    PtdIns-3-kinase C2 subunit alpha
    Alternative name(s):
    Phosphoinositide 3-kinase-C2-alpha
    Gene namesi
    Name:PIK3C2A
    OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
    Taxonomic identifieri9601 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
    ProteomesiUP000001595: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. clathrin-coated vesicle Source: UniProtKB-SubCell
    2. cytoplasm Source: UniProtKB
    3. Golgi apparatus Source: UniProtKB-SubCell
    4. nucleus Source: UniProtKB-SubCell
    5. plasma membrane Source: UniProtKB
    6. vesicle Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 16851684Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alphaPRO_0000088797Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei60 – 601PhosphoserineBy similarity
    Modified residuei108 – 1081PhosphoserineBy similarity
    Modified residuei259 – 2591PhosphoserineBy similarity
    Modified residuei327 – 3271PhosphoserineBy similarity
    Modified residuei338 – 3381PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated on Ser-259 during mitosis and upon UV irradiation; which does not change enzymatic activity but leads to proteasomal degradation. Phosphorylated upon insulin stimulation; which may lead to enzyme activation By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Interactioni

    Subunit structurei

    Part of a complex with ERBB2 and EGFR. Interacts with clathrin trimers By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ5RAY1.
    SMRiQ5RAY1. Positions 1419-1530, 1560-1681.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini419 – 50789PI3K-RBDPROSITE-ProRule annotationAdd
    BLAST
    Domaini680 – 839160C2 PI3K-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini859 – 1035177PIK helicalPROSITE-ProRule annotationAdd
    BLAST
    Domaini1131 – 1395265PI3K/PI4KPROSITE-ProRule annotationAdd
    BLAST
    Domaini1420 – 1536117PXPROSITE-ProRule annotationAdd
    BLAST
    Domaini1558 – 1661104C2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 142141Interaction with clathrin; sufficient to induce clathrin assembyBy similarityAdd
    BLAST
    Regioni1486 – 14916Interaction with PtdIns(4,5)P2-containing membranesBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi1607 – 161812Nuclear localization signalBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PI3/PI4-kinase family.PROSITE-ProRule annotation
    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 C2 PI3K-type domain.PROSITE-ProRule annotation
    Contains 1 PI3K-RBD domain.PROSITE-ProRule annotation
    Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation
    Contains 1 PIK helical domain.PROSITE-ProRule annotation
    Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOVERGENiHBG082099.
    InParanoidiQ5RAY1.
    KOiK00923.

    Family and domain databases

    Gene3Di1.10.1070.11. 1 hit.
    1.25.40.70. 1 hit.
    2.60.40.150. 2 hits.
    3.30.1520.10. 1 hit.
    InterProiIPR016024. ARM-type_fold.
    IPR000008. C2_dom.
    IPR011009. Kinase-like_dom.
    IPR001683. Phox.
    IPR000403. PI3/4_kinase_cat_dom.
    IPR018936. PI3/4_kinase_CS.
    IPR002420. PI3K_C2_dom.
    IPR000341. PI3K_Ras-bd_dom.
    IPR015433. PI_Kinase.
    IPR001263. PInositide-3_kin_accessory_dom.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PANTHERiPTHR10048. PTHR10048. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF00454. PI3_PI4_kinase. 1 hit.
    PF00792. PI3K_C2. 1 hit.
    PF00794. PI3K_rbd. 1 hit.
    PF00613. PI3Ka. 1 hit.
    PF00787. PX. 1 hit.
    [Graphical view]
    SMARTiSM00239. C2. 2 hits.
    SM00142. PI3K_C2. 1 hit.
    SM00144. PI3K_rbd. 1 hit.
    SM00145. PI3Ka. 1 hit.
    SM00146. PI3Kc. 1 hit.
    SM00312. PX. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.
    SSF49562. SSF49562. 2 hits.
    SSF54236. SSF54236. 1 hit.
    SSF56112. SSF56112. 1 hit.
    SSF64268. SSF64268. 1 hit.
    PROSITEiPS50004. C2. 1 hit.
    PS00915. PI3_4_KINASE_1. 1 hit.
    PS00916. PI3_4_KINASE_2. 1 hit.
    PS50290. PI3_4_KINASE_3. 1 hit.
    PS51547. PI3K_C2. 1 hit.
    PS51546. PI3K_RBD. 1 hit.
    PS51545. PIK_HELICAL. 1 hit.
    PS50195. PX. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5RAY1-1 [UniParc]FASTAAdd to Basket

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    MAQISSNSGF KECPSSHPEP TRAKDVDKEE ALQMEAEALA KLQKDRQVTD     50
    NQRGFELSSS TRKKAQVYNK QDYDLMVFPE SDSQKRALDI DVEKLTQAEL 100
    EKLLLDDSLE TRKTPVLPVT PILSPSFSAQ LYFRPTIQRG QWPPGLSGPS 150
    TYALPSIYPS TYSKQAAFQN GFNPRMPTFP STEPIYLSLP GQSPYFSYPL 200
    TPATPFHPQG SLPIYRPVVS PDMAKLFDKI ASTSEFLKNG KARADLEITD 250
    SKVSNLQVSP KSEDISKFDW LDLDPLSKPK VDNVEVLDHE EEKNVSSLLA 300
    KDPWDAVLLE ERSTANCHLE RKMNGKSLSV ATVTRSQSLN IRTTQLAKAH 350
    ISQKDPNGTS SLPTGSSLLQ EVEVQNEEMA AFSRSITKLK TKFPYTNHHT 400
    NPGYLLSPVT AQRNICGENA SVKVSIDIEG FQLPVTFTCD VSSTVEIIIM 450
    QALCWVHDDL NQVDVGSYVL KVCGQEEVLQ NNHCLGSHEH IQNCRKWDTE 500
    IRLQLLTFSA MCQNLARTAE DDETPVDLNK HLYQIEKPYK EAMTRHPVEE 550
    LLDSYHNQVE LALQIENQHR AVDQVIKAVR KICSALDGVE TLAITESVKK 600
    LKRAVNLPRS KTADVASLFG GEDTSKSSTR GSLNPENPVQ VSINQLTAAI 650
    YDLLRLHANS GRSPTDCAQS SKSVKEAWTT TEQLQFTIFA AHGISSNWVS 700
    NYEKYYLICS LSHNGKDLFK PIQSKKVGTY KNFFYLIKWD ELIIFPIQIS 750
    QLPLESLLHL TLFGILNQSS GSSPDSNKQR KGPEALGKVS LPLFDFKRFL 800
    TCGTKLLYLW TSSHTNSVPG AVTKKGYVME RIVLQVDFPS PAFDIIYTTP 850
    QVDRSIIQQH NLETLENDVK GKLLDILHKD SSLGLSKEDK AFLWEKRYYC 900
    FKHPNCLPKI LASAPNWKWV NLAKTYSLLH QWPALYPLIA LELLDSKFAD 950
    QEVRSLAVTW IEAISDDELT DLLPQFVQAL KYEIYLNSSL VQFLLSRALG 1000
    NIQIAHNLYW LLKDALHDVQ FSTRYEHVLG ALLSVGGKRL REELRKQTKL 1050
    VQLLGGVAEK VRQASGSARQ VVLQRSMERV QSFFQKNKCR LPLKPSLVAK 1100
    ELSIKSCSFF SSNAVPLKVT MVNADPMGEE INVMFKVGED LRQDMLALQM 1150
    IKIMDKIWLK EGLDLRMVIF KCLSTGRDRG MVELVPASDT LRKIQVEYGV 1200
    TGSFKDKPLA EWLRKYNPSE EEYEKASENF IYSCAGCCVA TYVLGICDRH 1250
    NDNIMLRSTG HMFHIDFGKF LGHAQMFGTF KRDRAPFVLT SDMAYVINGG 1300
    EKPTIRFQLF VDLCCQAYNL IRKQTNLFLN LLSLMIPSGL PELTSIQDLK 1350
    YVRDALQPQT TDAEATIFFT RLIESSLGSI ATKFNFLIHN LAQLRFSGLP 1400
    SNDEPILSFS PKTYSFKQDG RIKEVSVFTY HKKYNPDKHY IYVVRILREG 1450
    QIEPSFVFRT FDEFQELHNK LSIIFPLWKL PGFPNRMVLG RTHIKDVAAK 1500
    RKIELNSYLQ SLMNASTDVA ECDLVCTFFH PLLRDEKAEG IARSADAGSF 1550
    SPPTPGQIGG AVKLSISYRN GTLFIMVMHT KDLVTEDGAD PNPYVKTYLL 1600
    PDNHKTSKRK TKISRKTRNP TFNEMLVYSG YSKETLRQRE LQLSVLSAES 1650
    LRENFFLGGV TLPLKDFNLS KETVKWYQLT AATYL 1685
    Length:1,685
    Mass (Da):190,466
    Last modified:December 21, 2004 - v1
    Checksum:i2C48ED378DE0C2BD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR858880 mRNA. Translation: CAH91079.1.
    RefSeqiNP_001125626.1. NM_001132154.1.
    UniGeneiPab.799.

    Genome annotation databases

    GeneIDi100457125.
    KEGGipon:100457125.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR858880 mRNA. Translation: CAH91079.1 .
    RefSeqi NP_001125626.1. NM_001132154.1.
    UniGenei Pab.799.

    3D structure databases

    ProteinModelPortali Q5RAY1.
    SMRi Q5RAY1. Positions 1419-1530, 1560-1681.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100457125.
    KEGGi pon:100457125.

    Organism-specific databases

    CTDi 5286.

    Phylogenomic databases

    HOVERGENi HBG082099.
    InParanoidi Q5RAY1.
    KOi K00923.

    Family and domain databases

    Gene3Di 1.10.1070.11. 1 hit.
    1.25.40.70. 1 hit.
    2.60.40.150. 2 hits.
    3.30.1520.10. 1 hit.
    InterProi IPR016024. ARM-type_fold.
    IPR000008. C2_dom.
    IPR011009. Kinase-like_dom.
    IPR001683. Phox.
    IPR000403. PI3/4_kinase_cat_dom.
    IPR018936. PI3/4_kinase_CS.
    IPR002420. PI3K_C2_dom.
    IPR000341. PI3K_Ras-bd_dom.
    IPR015433. PI_Kinase.
    IPR001263. PInositide-3_kin_accessory_dom.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    PANTHERi PTHR10048. PTHR10048. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF00454. PI3_PI4_kinase. 1 hit.
    PF00792. PI3K_C2. 1 hit.
    PF00794. PI3K_rbd. 1 hit.
    PF00613. PI3Ka. 1 hit.
    PF00787. PX. 1 hit.
    [Graphical view ]
    SMARTi SM00239. C2. 2 hits.
    SM00142. PI3K_C2. 1 hit.
    SM00144. PI3K_rbd. 1 hit.
    SM00145. PI3Ka. 1 hit.
    SM00146. PI3Kc. 1 hit.
    SM00312. PX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    SSF49562. SSF49562. 2 hits.
    SSF54236. SSF54236. 1 hit.
    SSF56112. SSF56112. 1 hit.
    SSF64268. SSF64268. 1 hit.
    PROSITEi PS50004. C2. 1 hit.
    PS00915. PI3_4_KINASE_1. 1 hit.
    PS00916. PI3_4_KINASE_2. 1 hit.
    PS50290. PI3_4_KINASE_3. 1 hit.
    PS51547. PI3K_C2. 1 hit.
    PS51546. PI3K_RBD. 1 hit.
    PS51545. PIK_HELICAL. 1 hit.
    PS50195. PX. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. The German cDNA consortium
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.

    Entry informationi

    Entry nameiP3C2A_PONAB
    AccessioniPrimary (citable) accession number: Q5RAY1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2005
    Last sequence update: December 21, 2004
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3