Reviewed,
UniProtKB/Swiss-Prot Q5RAK8 (CLOCK_PONAB)
Last modified
February 9, 2010.
Version 43.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Circadian locomoter output cycles protein kaput EC=2.3.1.48 | ||
| Gene names |
| ||
| Organism | Pongo abelii (Sumatran orangutan) | ||
| Taxonomic identifier | 9601 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo |
Protein attributes
| Sequence length | 846 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | ARNTL/2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression. Acetylates primarily histones H3 and H4. Acetylates also a non-histone substrate: ARNTL By similarity. |
| Catalytic activity | Acetyl-CoA + [histone] = CoA + acetyl-[histone]. |
| Subunit structure | Component of the circadian clock oscillator which includes the CRY proteins, CLOCK or NPAS2, ARNTL or ARNTL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerization with ARNTL is required for E-box-dependent transactivation, for CLOCK nuclear translocation and degradation, and, for phosphorylation of both CLOCK and ARNTL. Interaction with PER and CRY proteins requires translocation to the nucleus. Interaction of the CLOCK-ARNTL heterodimer with PER or CRY inhibits transcription activation. Binds weakly ARNTL and ARNTL2 to form heterodimers which bind poorly to the E-box motif By similarity. |
| Subcellular location | Cytoplasm By similarity. Nucleus By similarity. Note: Shuffling between the cytoplasm and the nucleus is under circadian regulation and is ARNTL-dependent By similarity. |
| Post-translational modification | Phosphorylation is dependent on CLOCK-ARNTL heterodimer formation By similarity. |
| Sequence similarities | Contains 1 basic helix-loop-helix (bHLH) domain. Contains 1 PAC (PAS-associated C-terminal) domain. Contains 2 PAS (PER-ARNT-SIM) domains. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Biological rhythms Transcription Transcription regulation |
| Cellular component | Cytoplasm Nucleus |
| Domain | Repeat |
| Ligand | DNA-binding |
| Molecular function | Activator Transferase |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | signal transduction Inferred from electronic annotation. Source: InterPro transcriptionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | histone acetyltransferase activity Inferred from electronic annotation. Source: EC signal transducer activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 846 | 846 | Circadian locomoter output cycles protein kaput | PRO_0000262637 | |||||
Regions | |||||||||
| Domain | 48 – 85 | 38 | Helix-loop-helix motif | ||||||
| Domain | 107 – 177 | 71 | PAS 1 | ||||||
| Domain | 262 – 332 | 71 | PAS 2 | ||||||
| Domain | 336 – 379 | 44 | PAC | ||||||
| DNA binding | 35 – 47 | 13 | Basic motif | ||||||
| Region | 514 – 564 | 51 | Implicated in the circadian rhythmicity By similarity | ||||||
| Compositional bias | 483 – 828 | 346 | Gln-rich | ||||||
Amino acid modifications | |||||||||
| Modified residue | 408 | 1 | Phosphoserine By similarity | ||||||
Sequences
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References
| [1] | The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | CR859007 mRNA. Translation: CAH91202.1. |
| RefSeq | NP_001125706.1. |
| UniGene | Pab.7567 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1AM9 based on UniProtKB P36956. |
| SMR | Q5RAK8. Positions 32-86, 119-380. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 100172630. |
Organism-specific databases | |
| CTD | 100172630. |
Phylogenomic databases | |
| HOVERGEN | Q5RAK8. |
| InParanoid | Q5RAK8. |
Enzyme and pathway databases | |
| BRENDA | 2.3.1.48. 269192. |
Family and domain databases | |
| InterPro | IPR001092. HLH_DNA-bd_dom. IPR011598. HLH_DNA_bd. IPR001067. Nuc_translocat. IPR001610. PAC. IPR000014. PAS. IPR013767. PAS_fold. IPR013655. PAS_fold_3. [Graphical view] |
| Gene3D | G3DSA:4.10.280.10. HLH_DNA_bd. 1 hit. |
| Pfam | PF00010. HLH. 1 hit. PF00989. PAS. 1 hit. PF08447. PAS_3. 1 hit. [Graphical view] |
| PRINTS | PR00785. NCTRNSLOCATR. |
| SMART | SM00353. HLH. 1 hit. SM00086. PAC. 1 hit. SM00091. PAS. 2 hits. [Graphical view] |
| PROSITE | PS50888. HLH. 1 hit. PS50113. PAC. False negative. PS50112. PAS. 2 hits. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | CLOCK_PONAB | ||||||||
| Accession | Primary (citable) accession number: Q5RAK8 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

Clusters with


