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Q5RAJ7

- NEIL2_PONAB

UniProt

Q5RAJ7 - NEIL2_PONAB

Protein

Endonuclease 8-like 2

Gene

NEIL2

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Has DNA glycosylase activity towards 5-hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double-stranded DNA (DNA bubbles). Has low or no DNA glycosylase activity towards thymine glycol, 2-hydroxyadenine, hypoxanthine and 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates By similarity.By similarity

    Catalytic activityi

    Removes damaged bases from DNA, leaving an abasic site.
    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.PROSITE-ProRule annotation

    Enzyme regulationi

    Acetylation of Lys-50 leads to loss of DNA nicking activity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21Schiff-base intermediate with DNAPROSITE-ProRule annotation
    Active sitei3 – 31Proton donorPROSITE-ProRule annotation
    Active sitei50 – 501Proton donor; for beta-elimination activityPROSITE-ProRule annotation
    Binding sitei231 – 2311DNABy similarity
    Active sitei310 – 3101Proton donor; for delta-elimination activityPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri284 – 32037FPG-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. damaged DNA binding Source: InterPro
    2. DNA-(apurinic or apyrimidinic site) lyase activity Source: UniProtKB-EC
    3. hydrolase activity, hydrolyzing N-glycosyl compounds Source: InterPro
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. base-excision repair Source: InterPro
    2. nucleotide-excision repair Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Lyase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endonuclease 8-like 2 (EC:3.2.2.-, EC:4.2.99.18)
    Alternative name(s):
    DNA glycosylase/AP lyase Neil2
    DNA-(apurinic or apyrimidinic site) lyase Neil2
    Endonuclease VIII-like 2
    Nei-like protein 2
    Gene namesi
    Name:NEIL2
    OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
    Taxonomic identifieri9601 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
    ProteomesiUP000001595: Unplaced

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 332331Endonuclease 8-like 2PRO_0000248635Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei50 – 501N6-acetyllysineBy similarity
    Modified residuei154 – 1541N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Interactioni

    Subunit structurei

    Binds EP300.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ5RAJ7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    The zinc-finger domain is important for DNA binding.

    Sequence similaritiesi

    Belongs to the FPG family.PROSITE-ProRule annotation
    Contains 1 FPG-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri284 – 32037FPG-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    HOVERGENiHBG082014.
    InParanoidiQ5RAJ7.
    KOiK10568.

    Family and domain databases

    InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
    IPR012319. DNA_glycosylase/AP_lyase_cat.
    IPR010979. Ribosomal_S13-like_H2TH.
    IPR000214. Znf_DNA_glyclase/AP_lyase.
    [Graphical view]
    PfamiPF01149. Fapy_DNA_glyco. 1 hit.
    PF06831. H2TH. 1 hit.
    [Graphical view]
    SUPFAMiSSF46946. SSF46946. 1 hit.
    PROSITEiPS51068. FPG_CAT. 1 hit.
    PS51066. ZF_FPG_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5RAJ7-1 [UniParc]FASTAAdd to Basket

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    MPEGPLVRKF HHLVSPFVGQ QVVKTGGSSK KLQPASLQCL WLQDTQVNGK    50
    KLFLRFDPDE EMGPPGSSPP PEPPQKEAQK EGAADPKQVG EPSGQKTPDG 100
    SSQSAELVPQ GEDDSEYLER DAPAGDAGRW LRVSFGLFGS VWVNEFSRAK 150
    QANKRGDWRD PSPRLVLHCG GGGFLAFYNC QMSWSSSPVV TPTCDILSEK 200
    FHRGQALEAL GQAQPVCYTL LDQRYFSGLG NIIKNEALYR AGIHPLSLGS 250
    VLSASRREVL VDHVVEFSTA WLQGKFQGRP QRTQVYQREQ CPAGHQVMKE 300
    AFGPQDGLQR LTWWCPQCQP QLSEEPEQRQ FS 332
    Length:332
    Mass (Da):36,814
    Last modified:January 23, 2007 - v3
    Checksum:i607670A7EFE6E6C5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR859018 mRNA. Translation: CAH91213.1.
    RefSeqiNP_001125714.1. NM_001132242.1.

    Genome annotation databases

    GeneIDi100172638.
    KEGGipon:100172638.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR859018 mRNA. Translation: CAH91213.1 .
    RefSeqi NP_001125714.1. NM_001132242.1.

    3D structure databases

    ProteinModelPortali Q5RAJ7.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100172638.
    KEGGi pon:100172638.

    Organism-specific databases

    CTDi 252969.

    Phylogenomic databases

    HOVERGENi HBG082014.
    InParanoidi Q5RAJ7.
    KOi K10568.

    Family and domain databases

    InterProi IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
    IPR012319. DNA_glycosylase/AP_lyase_cat.
    IPR010979. Ribosomal_S13-like_H2TH.
    IPR000214. Znf_DNA_glyclase/AP_lyase.
    [Graphical view ]
    Pfami PF01149. Fapy_DNA_glyco. 1 hit.
    PF06831. H2TH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46946. SSF46946. 1 hit.
    PROSITEi PS51068. FPG_CAT. 1 hit.
    PS51066. ZF_FPG_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. The German cDNA consortium
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.

    Entry informationi

    Entry nameiNEIL2_PONAB
    AccessioniPrimary (citable) accession number: Q5RAJ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 49 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3