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Q5RAF4

- NEUR1_PONAB

UniProt

Q5RAF4 - NEUR1_PONAB

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Protein
Sialidase-1
Gene
NEU1
Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage By similarity.

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei78 – 781Substrate By similarity
Binding sitei97 – 971Substrate By similarity
Active sitei103 – 1031Proton acceptor By similarity
Binding sitei264 – 2641Substrate By similarity
Binding sitei280 – 2801Substrate By similarity
Binding sitei341 – 3411Substrate By similarity
Active sitei370 – 3701Nucleophile By similarity
Active sitei394 – 3941 Reviewed prediction

GO - Molecular functioni

  1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
  2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
  3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC
  4. exo-alpha-sialidase activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. lipid catabolic process Source: UniProtKB-KW
  2. oligosaccharide catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Lipid degradation, Lipid metabolism

Protein family/group databases

CAZyiGH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase-1 (EC:3.2.1.18)
Alternative name(s):
Acetylneuraminyl hydrolase
Lysosomal sialidase
N-acetyl-alpha-neuraminidase 1
Gene namesi
Name:NEU1
OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Taxonomic identifieri9601 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
ProteomesiUP000001595: Unplaced

Subcellular locationi

Lysosome membrane; Peripheral membrane protein; Lumenal side By similarity. Lysosome lumen By similarity. Cell membrane By similarity. Cytoplasmic vesicle By similarity
Note: Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles By similarity.

GO - Cellular componenti

  1. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
  2. lysosomal lumen Source: UniProtKB-SubCell
  3. lysosomal membrane Source: UniProtKB-SubCell
  4. lysosome Source: UniProtKB
  5. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4747 By similarity
Add
BLAST
Chaini48 – 415368Sialidase-1
PRO_0000304728Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi186 – 1861N-linked (GlcNAc...) Reviewed prediction
Glycosylationi343 – 3431N-linked (GlcNAc...) Reviewed prediction
Glycosylationi352 – 3521N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

N-glycosylated By similarity.
Phosphorylation of tyrosine within the internalization signal results in inhibition of sialidase internalization and blockage on the plasma membrane By similarity.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiQ5RAF4.

Interactioni

Subunit structurei

Interacts with cathepsin A (protective protein), beta-galactosidase and N-acetylgalactosamine-6-sulfate sulfatase in a multienzyme complex By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ5RAF4.
SMRiQ5RAF4. Positions 55-396.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati112 – 12312BNR 1
Add
BLAST
Repeati172 – 18312BNR 2
Add
BLAST
Repeati231 – 24212BNR 3
Add
BLAST
Repeati347 – 35812BNR 4
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi77 – 804FRIP motif
Motifi412 – 4154Internalization signal

Domaini

A C-terminal internalization signal (YGTL) appears to allow the targeting of plasma membrane proteins to endosomes.

Sequence similaritiesi

Contains 4 BNR repeats.

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOVERGENiHBG057314.
InParanoidiQ5RAF4.
KOiK01186.

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
SUPFAMiSSF50939. SSF50939. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q5RAF4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MTGERPSTAL PDRRWGPRIL GFWGGCRVWV FAAIFLLLSL AASWSKAEND    50
FGLVQPLVTM EQLLWVSGRQ IGSVDTFRIP LITATPRGTL LAFAEARKMS 100
SSDEGAKFIA LRRSMDQGST WSPTAFIVND GDVPDGLNLG AVVSDVETGV 150
VFLFYSLCAH KAGCQVASTM LVWSKDDGVS WSTPRNLSLD IGTEVFAPGP 200
GSGIQKQREP RKGRLIVCGH GTLERDGVFC LLSDDHGASW RYGSGVSGIP 250
YGQPKQENDF NPDECQPYEL PDGSVVINAR NQNNYHCHCR IVLRSYDACD 300
TLRPRDVTFD PELVDPVVAA GAVVTSSGIV FFSNPAHPEF RVNLTLRWSF 350
SNGTSWRKET VQLWPGPSGY SSLATLEGSM DGEEQAPQLY VLYEKGRNHY 400
TESISVAKIS VYGTL 415
Length:415
Mass (Da):45,467
Last modified:December 21, 2004 - v1
Checksum:i360E60A256DEA07F
GO
Isoform 2 (identifier: Q5RAF4-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     267-414: Missing.

Note: No experimental confirmation available.

Show »
Length:267
Mass (Da):29,000
Checksum:iFFE761254DE87C3A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei267 – 414148Missing in isoform 2.
VSP_028119Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51R → Q in CAH89635. 1 Publication
Sequence conflicti5 – 51R → Q in CAH91396. 1 Publication
Sequence conflicti12 – 121D → G in CAH89635. 1 Publication
Sequence conflicti12 – 121D → G in CAH91396. 1 Publication
Sequence conflicti29 – 291W → R in CAH89635. 1 Publication
Sequence conflicti29 – 291W → R in CAH91396. 1 Publication
Sequence conflicti57 – 571L → P in CAH91396. 1 Publication
Sequence conflicti256 – 2561Q → H in CAH89635. 1 Publication
Sequence conflicti256 – 2561Q → H in CAH91396. 1 Publication
Sequence conflicti344 – 3441L → P in CAH89635. 1 Publication
Sequence conflicti406 – 4061V → M in CAH89635. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR857339 mRNA. Translation: CAH89635.1.
CR859063 mRNA. Translation: CAH91256.1.
CR859212 mRNA. Translation: CAH91396.1.
RefSeqiNP_001125743.1. NM_001132271.1. [Q5RAF4-1]
NP_001128939.1. NM_001135467.1.

Genome annotation databases

GeneIDi100172668.
KEGGipon:100172668.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR857339 mRNA. Translation: CAH89635.1 .
CR859063 mRNA. Translation: CAH91256.1 .
CR859212 mRNA. Translation: CAH91396.1 .
RefSeqi NP_001125743.1. NM_001132271.1. [Q5RAF4-1 ]
NP_001128939.1. NM_001135467.1.

3D structure databases

ProteinModelPortali Q5RAF4.
SMRi Q5RAF4. Positions 55-396.
ModBasei Search...

Chemistry

BindingDBi Q5RAF4.

Protein family/group databases

CAZyi GH33. Glycoside Hydrolase Family 33.

Proteomic databases

PRIDEi Q5RAF4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100172668.
KEGGi pon:100172668.

Organism-specific databases

CTDi 4758.

Phylogenomic databases

HOVERGENi HBG057314.
InParanoidi Q5RAF4.
KOi K01186.

Family and domain databases

Gene3Di 2.120.10.10. 1 hit.
InterProi IPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view ]
PANTHERi PTHR10628. PTHR10628. 1 hit.
SUPFAMi SSF50939. SSF50939. 1 hit.
ProtoNeti Search...

Publicationsi

  1. The German cDNA consortium
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain cortex, Heart and Kidney.

Entry informationi

Entry nameiNEUR1_PONAB
AccessioniPrimary (citable) accession number: Q5RAF4
Secondary accession number(s): Q5RA14, Q5RF22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: December 21, 2004
Last modified: June 11, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi