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Q5RAF4

- NEUR1_PONAB

UniProt

Q5RAF4 - NEUR1_PONAB

Protein

Sialidase-1

Gene

NEU1

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (21 Dec 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage By similarity.By similarity

    Catalytic activityi

    Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei78 – 781SubstrateBy similarity
    Binding sitei97 – 971SubstrateBy similarity
    Active sitei103 – 1031Proton acceptorBy similarity
    Binding sitei264 – 2641SubstrateBy similarity
    Binding sitei280 – 2801SubstrateBy similarity
    Binding sitei341 – 3411SubstrateBy similarity
    Active sitei370 – 3701NucleophileBy similarity
    Active sitei394 – 3941Sequence Analysis

    GO - Molecular functioni

    1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
    2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
    3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC
    4. exo-alpha-sialidase activity Source: UniProtKB

    GO - Biological processi

    1. lipid catabolic process Source: UniProtKB-KW
    2. oligosaccharide catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Lipid degradation, Lipid metabolism

    Protein family/group databases

    CAZyiGH33. Glycoside Hydrolase Family 33.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sialidase-1 (EC:3.2.1.18)
    Alternative name(s):
    Acetylneuraminyl hydrolase
    Lysosomal sialidase
    N-acetyl-alpha-neuraminidase 1
    Gene namesi
    Name:NEU1
    OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
    Taxonomic identifieri9601 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
    ProteomesiUP000001595: Unplaced

    Subcellular locationi

    Lysosome membrane By similarity; Peripheral membrane protein By similarity; Lumenal side By similarity. Lysosome lumen By similarity. Cell membrane By similarity. Cytoplasmic vesicle By similarity
    Note: Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles.By similarity

    GO - Cellular componenti

    1. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
    2. lysosomal lumen Source: UniProtKB-SubCell
    3. lysosomal membrane Source: UniProtKB-SubCell
    4. lysosome Source: UniProtKB
    5. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasmic vesicle, Lysosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 4747By similarityAdd
    BLAST
    Chaini48 – 415368Sialidase-1PRO_0000304728Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi186 – 1861N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi343 – 3431N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi352 – 3521N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated.By similarity
    Phosphorylation of tyrosine within the internalization signal results in inhibition of sialidase internalization and blockage on the plasma membrane.By similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiQ5RAF4.

    Interactioni

    Subunit structurei

    Interacts with cathepsin A (protective protein), beta-galactosidase and N-acetylgalactosamine-6-sulfate sulfatase in a multienzyme complex.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ5RAF4.
    SMRiQ5RAF4. Positions 55-396.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati112 – 12312BNR 1Add
    BLAST
    Repeati172 – 18312BNR 2Add
    BLAST
    Repeati231 – 24212BNR 3Add
    BLAST
    Repeati347 – 35812BNR 4Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi77 – 804FRIP motif
    Motifi412 – 4154Internalization signal

    Domaini

    A C-terminal internalization signal (YGTL) appears to allow the targeting of plasma membrane proteins to endosomes.

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 33 family.Curated
    Contains 4 BNR repeats.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    HOVERGENiHBG057314.
    InParanoidiQ5RAF4.
    KOiK01186.

    Family and domain databases

    Gene3Di2.120.10.10. 1 hit.
    InterProiIPR026856. Sialidase_fam.
    IPR011040. Sialidases.
    [Graphical view]
    PANTHERiPTHR10628. PTHR10628. 1 hit.
    SUPFAMiSSF50939. SSF50939. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q5RAF4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTGERPSTAL PDRRWGPRIL GFWGGCRVWV FAAIFLLLSL AASWSKAEND    50
    FGLVQPLVTM EQLLWVSGRQ IGSVDTFRIP LITATPRGTL LAFAEARKMS 100
    SSDEGAKFIA LRRSMDQGST WSPTAFIVND GDVPDGLNLG AVVSDVETGV 150
    VFLFYSLCAH KAGCQVASTM LVWSKDDGVS WSTPRNLSLD IGTEVFAPGP 200
    GSGIQKQREP RKGRLIVCGH GTLERDGVFC LLSDDHGASW RYGSGVSGIP 250
    YGQPKQENDF NPDECQPYEL PDGSVVINAR NQNNYHCHCR IVLRSYDACD 300
    TLRPRDVTFD PELVDPVVAA GAVVTSSGIV FFSNPAHPEF RVNLTLRWSF 350
    SNGTSWRKET VQLWPGPSGY SSLATLEGSM DGEEQAPQLY VLYEKGRNHY 400
    TESISVAKIS VYGTL 415
    Length:415
    Mass (Da):45,467
    Last modified:December 21, 2004 - v1
    Checksum:i360E60A256DEA07F
    GO
    Isoform 2 (identifier: Q5RAF4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         267-414: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:267
    Mass (Da):29,000
    Checksum:iFFE761254DE87C3A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti5 – 51R → Q in CAH89635. 1 PublicationCurated
    Sequence conflicti5 – 51R → Q in CAH91396. 1 PublicationCurated
    Sequence conflicti12 – 121D → G in CAH89635. 1 PublicationCurated
    Sequence conflicti12 – 121D → G in CAH91396. 1 PublicationCurated
    Sequence conflicti29 – 291W → R in CAH89635. 1 PublicationCurated
    Sequence conflicti29 – 291W → R in CAH91396. 1 PublicationCurated
    Sequence conflicti57 – 571L → P in CAH91396. 1 PublicationCurated
    Sequence conflicti256 – 2561Q → H in CAH89635. 1 PublicationCurated
    Sequence conflicti256 – 2561Q → H in CAH91396. 1 PublicationCurated
    Sequence conflicti344 – 3441L → P in CAH89635. 1 PublicationCurated
    Sequence conflicti406 – 4061V → M in CAH89635. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei267 – 414148Missing in isoform 2. 1 PublicationVSP_028119Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR857339 mRNA. Translation: CAH89635.1.
    CR859063 mRNA. Translation: CAH91256.1.
    CR859212 mRNA. Translation: CAH91396.1.
    RefSeqiNP_001125743.1. NM_001132271.1. [Q5RAF4-1]
    NP_001128939.1. NM_001135467.1.

    Genome annotation databases

    GeneIDi100172668.
    KEGGipon:100172668.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR857339 mRNA. Translation: CAH89635.1 .
    CR859063 mRNA. Translation: CAH91256.1 .
    CR859212 mRNA. Translation: CAH91396.1 .
    RefSeqi NP_001125743.1. NM_001132271.1. [Q5RAF4-1 ]
    NP_001128939.1. NM_001135467.1.

    3D structure databases

    ProteinModelPortali Q5RAF4.
    SMRi Q5RAF4. Positions 55-396.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi Q5RAF4.

    Protein family/group databases

    CAZyi GH33. Glycoside Hydrolase Family 33.

    Proteomic databases

    PRIDEi Q5RAF4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100172668.
    KEGGi pon:100172668.

    Organism-specific databases

    CTDi 4758.

    Phylogenomic databases

    HOVERGENi HBG057314.
    InParanoidi Q5RAF4.
    KOi K01186.

    Family and domain databases

    Gene3Di 2.120.10.10. 1 hit.
    InterProi IPR026856. Sialidase_fam.
    IPR011040. Sialidases.
    [Graphical view ]
    PANTHERi PTHR10628. PTHR10628. 1 hit.
    SUPFAMi SSF50939. SSF50939. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. The German cDNA consortium
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Brain cortex, Heart and Kidney.

    Entry informationi

    Entry nameiNEUR1_PONAB
    AccessioniPrimary (citable) accession number: Q5RAF4
    Secondary accession number(s): Q5RA14, Q5RF22
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: December 21, 2004
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3