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Protein

Arginine--tRNA ligase, cytoplasmic

Gene

RARS

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1.By similarity

Catalytic activityi

ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei211L-arginineBy similarity1
Binding sitei384L-arginineBy similarity1
Binding sitei388L-arginineBy similarity1
Binding sitei412L-arginineBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Arginine--tRNA ligase, cytoplasmic (EC:6.1.1.19By similarity)
Alternative name(s):
Arginyl-tRNA synthetase
Short name:
ArgRS
Gene namesi
Name:RARS
OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Taxonomic identifieri9601 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
Proteomesi
  • UP000001595 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002507281 – 660Arginine--tRNA ligase, cytoplasmicAdd BLAST660

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiQ5RA20.

Interactioni

Subunit structurei

Interacts (via N-terminus) with AIMP1 (via N-terminus); this stimulates its catalytic activity. Interacts (via N-terminus) with LARS2 (via C-terminus). Monomer. Part of a multisubunit complex that groups tRNA ligases for Arg (RARS), Asp (DARS), Gln (QARS), Ile (IARS), Leu (LARS), Lys (KARS), Met (MARS) the bifunctional ligase for Glu and Pro (EPRS) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18. Interacts with QARS. Part of a complex composed of RARS, QARS and AIMP1.By similarity

Protein-protein interaction databases

STRINGi9601.ENSPPYP00000017935.

Structurei

3D structure databases

ProteinModelPortaliQ5RA20.
SMRiQ5RA20.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 72Could be involved in the assembly of the multisynthetase complexAdd BLAST72
Regioni200 – 202L-arginine bindingBy similarity3
Regioni529 – 543Interaction with tRNABy similarityAdd BLAST15

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi201 – 212"HIGH" regionAdd BLAST12

Domaini

The alpha-helical N-terminus (residues 1-72) mediates interaction with AIMP1 and thereby contributes to the assembly of the multisynthetase complex.By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1195. Eukaryota.
COG0018. LUCA.
HOVERGENiHBG029238.
InParanoidiQ5RA20.
KOiK01887.

Family and domain databases

CDDicd00671. ArgRS_core. 1 hit.
Gene3Di3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00123. Arg_tRNA_synth. 1 hit.
InterProiView protein in InterPro
IPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR036695. Arg-tRNA-synth_N_sf.
IPR035684. ArgRS_core.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
PANTHERiPTHR11956. PTHR11956. 1 hit.
PfamiView protein in Pfam
PF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
PRINTSiPR01038. TRNASYNTHARG.
SMARTiView protein in SMART
SM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsiTIGR00456. argS. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5RA20-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVLVTECSA RLLQQEEEIK SLTAEIDRLK NCGCLGASAN LEQLQEENLK
60 70 80 90 100
LKYRLNILQK SLQAERNKPT KNMINVISRL QEVFGHAIKA AYPDLENPPL
110 120 130 140 150
LVTPSQQAKF GDYQCNSAMG ISQMLKTKEQ KVNPREIAEN ITKHLPDNEC
160 170 180 190 200
IEKVEIAGPG FINIHLRKDF VSEQLTSLLV NGVQLPALGE NKKVIVDFSS
210 220 230 240 250
PNIAKEMHVG HLRSTIIGES ISRLFEFAGY DVLRLNHIGD WGTQFGMLIA
260 270 280 290 300
HLQDKFPDYL TVSPPIGDLQ VFYKESKKRF DTEEEFKKRA YQCVVLLQGK
310 320 330 340 350
NPDITKAWKL ICDVSRQELN KIYDALDVSL IERGESFYQD MMNDIVKEFE
360 370 380 390 400
DRGFVQVDDG RKIVFVPGCS IPLTILKSDG GYTYDTSDLA AIKQRLFEEK
410 420 430 440 450
ADMIIYVVDN GQSVHFQTIF AAAQMIGWYD PKVTRVFHAG FGVVLGEDKK
460 470 480 490 500
KFKTRSGETV RLMDLLGEGL KRSMDKLKEK ERDKVLTAEE LNAAQTSIAY
510 520 530 540 550
GCVKYADLSH NRLNDYIFSF DKMLDDKGNT AAYLLYAFTR IRSIARLANI
560 570 580 590 600
DEEMLQKAAR ETKILLDHEK EWKLGRCILR FPEILQKILD DLFLHTLCDY
610 620 630 640 650
IYELATTFTE FYDSCYCVEK DRQTGKILKV NMWRMLLCEA VAAVMAKGFD
660
ILGIKPVQRM
Length:660
Mass (Da):75,344
Last modified:December 21, 2004 - v1
Checksum:iC114CA5ED797D8E8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti164I → V in CAI29693 (Ref. 1) Curated1
Sequence conflicti271V → A in CAI29693 (Ref. 1) Curated1
Sequence conflicti402Missing in CAI29693 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR859203 mRNA. Translation: CAH91390.1.
CR926065 mRNA. Translation: CAI29693.1.
RefSeqiNP_001127107.1. NM_001133635.1.

Genome annotation databases

GeneIDi100174147.
KEGGipon:100174147.

Similar proteinsi

Entry informationi

Entry nameiSYRC_PONAB
AccessioniPrimary (citable) accession number: Q5RA20
Secondary accession number(s): Q5NVH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: December 21, 2004
Last modified: November 22, 2017
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families