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Q5R9C1 (F263_PONAB) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
Short name=6PF-2-K/Fru-2,6-P2ase 3
Short name=PFK/FBPase 3
Alternative name(s):
6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme

Including the following 2 domains:

  1. 6-phosphofructo-2-kinase
    EC=2.7.1.105
  2. Fructose-2,6-bisphosphatase
    EC=3.1.3.46
Gene names
Name:PFKFB3
OrganismPongo abelii (Sumatran orangutan)
Taxonomic identifier9601 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length514 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Synthesis and degradation of fructose 2,6-bisphosphate By similarity.

Catalytic activity

Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.

ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.

Post-translational modification

Phosphorylation by AMPK stimulates activity By similarity.

Sequence similarities

In the C-terminal section; belongs to the phosphoglycerate mutase family.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q5R9C1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q5R9C1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     3-26: LELTQSRVQKIWVPVDHRPSLPRS → FRKA
     506-514: PLLGQACLT → NMKGSRSSADSSRKH
Isoform 3 (identifier: Q5R9C1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     3-26: LELTQSRVQKIWVPVDHRPSLPRS → FRKA
     514-514: T → RTVCHIFSKFSPY

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5145146-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
PRO_0000345149

Regions

Nucleotide binding42 – 498ATP By similarity
Region1 – 2452456-phosphofructo-2-kinase
Region246 – 514269Fructose-2,6-bisphosphatase

Sites

Active site1251 Potential
Active site1551 Potential
Active site2541Tele-phosphohistidine intermediate By similarity
Active site3231 Potential
Active site3881Proton donor By similarity
Binding site991Fructose-6-phosphate By similarity
Binding site1901Fructose-6-phosphate By similarity

Amino acid modifications

Modified residue4611Phosphoserine; by AMPK and PKA By similarity
Modified residue4631Phosphothreonine By similarity
Modified residue4671Phosphoserine By similarity
Modified residue4711Phosphothreonine; by PKC By similarity

Natural variations

Alternative sequence3 – 2624LELTQ…SLPRS → FRKA in isoform 2 and isoform 3.
VSP_034919
Alternative sequence506 – 5149PLLGQACLT → NMKGSRSSADSSRKH in isoform 2.
VSP_034920
Alternative sequence5141T → RTVCHIFSKFSPY in isoform 3.
VSP_034921

Experimental info

Sequence conflict371V → A in CAH92952. Ref.1
Sequence conflict2241V → A in CAH93512. Ref.1
Sequence conflict3261A → V in CAH91639. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 22, 2008. Version 2.
Checksum: 18FD1D267306853D

FASTA51458,876
        10         20         30         40         50         60 
MPLELTQSRV QKIWVPVDHR PSLPRSCGPK LTNSPTVIVM VGLPARGKTY ISKKLTRYLN 

        70         80         90        100        110        120 
WIGVPTKVFN VGEYRREAVK QYSSYNFFRP DNEEAMKVRK QCALAALRDV KSYLAKEGGQ 

       130        140        150        160        170        180 
IAVFDATNTT RERRHMILHF AKENDFKAFF IESVCDDPTV VASNIMEVKI SSPDYKDCNS 

       190        200        210        220        230        240 
AEAMDDFMKR ISCYEASYQP LDPDKCDRDL SLIKVIDVGR RFLVNRVQDH IQSRIVYYLM 

       250        260        270        280        290        300 
NIHVQPRTIY LCRHGENEHN LQGRIGGDSG LSSRGKKFAS ALSKFVEEQN LKDLRVWTSQ 

       310        320        330        340        350        360 
LKSTIQTAEA LRLPYEQWKA LNEIDAGVCE ELTYEEIRDT YPEEYALREQ DKYYYRYPTG 

       370        380        390        400        410        420 
ESYQDLVQRL EPVIMELERQ ENVLVICHQA VLRCLLAYFL DKSAEEMPYL KCPLHTVLKL 

       430        440        450        460        470        480 
TPVAYGCRVE SIYLNVESVC THRERSEDAK KGPNPLMRRN SVTPLASPEP TKKPRINSFE 

       490        500        510 
EHVASTSAAL PSCLPPEVPT QLPGQPLLGQ ACLT

« Hide

Isoform 2 [UniParc].

Checksum: 32D2924D3D207C17
Show »

FASTA50057,287
Isoform 3 [UniParc].

Checksum: 3481133B523AEDB6
Show »

FASTA50658,021

References

[1]The German cDNA consortium
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
Tissue: Brain cortex and Kidney.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CR859468 mRNA. Translation: CAH91639.1.
CR860843 mRNA. Translation: CAH92952.1.
CR861456 mRNA. Translation: CAH93512.1.
RefSeqNP_001125963.1. NM_001132491.1.
NP_001128959.1. NM_001135487.1.
UniGenePab.18003.

3D structure databases

HSSPHSSP built from PDB template 2AXN based on UniProtKB Q16875.
ProteinModelPortalQ5R9C1.
SMRQ5R9C1. Positions 3-461.
ModBaseSearch...

Proteomic databases

PRIDEQ5R9C1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSPPYT00000002466; ENSPPYP00000002394; ENSPPYG00000002068.
GeneID100172898.
KEGGpon:100172898.

Organism-specific databases

CTD5209.

Phylogenomic databases

HOVERGENHBG005628.

Family and domain databases

InterProIPR003094. 6Pfruct_kin.
IPR013079. 6Phosfructo_kin.
IPR016260. Bifunct_6PFK/fruc_bisP_Ptase.
IPR013078. His_Pase_superF_clade-1.
IPR001345. PG/BPGM_mutase_AS.
[Graphical view]
KOK01103.
PANTHERPTHR10606. 6Pfruct_kin. 1 hit.
PfamPF01591. 6PF2K. 1 hit.
PF00300. His_Phos_1. 1 hit.
[Graphical view]
PIRSFPIRSF000709. 6PFK_2-Ptase. 1 hit.
PRINTSPR00991. 6PFRUCTKNASE.
SMARTSM00855. PGAM. 1 hit.
[Graphical view]
PROSITEPS00175. PG_MUTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF263_PONAB
AccessionPrimary (citable) accession number: Q5R9C1
Secondary accession number(s): Q5R404, Q5R5L4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: July 22, 2008
Last modified: November 16, 2011
This is version 36 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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