Reviewed, UniProtKB/Swiss-Prot Q5R9C1 (F263_PONAB)

Names and origin

Protein names

Recommended name:
    6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
Alternative name(s):
    6PF-2-K/Fru-2,6-P2ASE brain/placenta-type isozyme
Including the following 2 domains:
    1- Recommended name:
            6-phosphofructo-2-kinase
              EC=2.7.1.105
    2- Recommended name:
            Fructose-2,6-bisphosphatase
              EC=3.1.3.46

Gene names

Name:

PFKFB3

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Synthesis and degradation of fructose 2,6-bisphosphate By similarity.
Catalytic activity	Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.
Sequence similarities	In the C-terminal section; belongs to the phosphoglycerate mutase family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Kinase Transferase
PTM	Phosphoprotein
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	fructose 2,6-bisphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructo-2-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW fructose-2,6-bisphosphate 2-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q5R9C1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q5R9C1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     3-26: LELTQSRVQKIWVPVDHRPSLPRS → FRKA
     506-514: PLLGQACLT → NMKGSRSSADSSRKH

Isoform 3 (identifier: Q5R9C1-3)

The sequence of this isoform differs from the canonical sequence as follows:
     3-26: LELTQSRVQKIWVPVDHRPSLPRS → FRKA
     514-514: T → RTVCHIFSKFSPY

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 514	514	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3		PRO_0000345149
Regions
	Nucleotide binding	42 – 49	8	ATP By similarity
	Region	1 – 245	245	6-phosphofructo-2-kinase
	Region	246 – 514	269	Fructose-2,6-bisphosphatase
Sites
	Active site	125	1	Potential
	Active site	155	1	Potential
	Active site	254	1	Tele-phosphohistidine intermediate By similarity
	Active site	323	1	Potential
	Active site	388	1	Proton donor By similarity
	Binding site	99	1	Fructose-6-phosphate By similarity
	Binding site	190	1	Fructose-6-phosphate By similarity
Amino acid modifications
	Modified residue	461	1	Phosphoserine; by PKA By similarity
	Modified residue	463	1	Phosphothreonine By similarity
	Modified residue	467	1	Phosphoserine By similarity
	Modified residue	471	1	Phosphothreonine; by PKC By similarity
Natural variations
	Alternative sequence	3 – 26	24	LELTQ…SLPRS → FRKA in isoform 2 and isoform 3.		VSP_034919
	Alternative sequence	506 – 514	9	PLLGQACLT → NMKGSRSSADSSRKH in isoform 2.		VSP_034920
	Alternative sequence	514	1	T → RTVCHIFSKFSPY in isoform 3.		VSP_034921
Experimental info
	Sequence conflict	37	1	V → A in CAH92952. Ref.1
	Sequence conflict	224	1	V → A in CAH93512. Ref.1
	Sequence conflict	326	1	A → V in CAH91639. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 22, 2008. Version 2. Checksum: 18FD1D267306853D	FASTA	514	58,876
	10 20 30 40 50 60 MPLELTQSRV QKIWVPVDHR PSLPRSCGPK LTNSPTVIVM VGLPARGKTY ISKKLTRYLN 70 80 90 100 110 120 WIGVPTKVFN VGEYRREAVK QYSSYNFFRP DNEEAMKVRK QCALAALRDV KSYLAKEGGQ 130 140 150 160 170 180 IAVFDATNTT RERRHMILHF AKENDFKAFF IESVCDDPTV VASNIMEVKI SSPDYKDCNS 190 200 210 220 230 240 AEAMDDFMKR ISCYEASYQP LDPDKCDRDL SLIKVIDVGR RFLVNRVQDH IQSRIVYYLM 250 260 270 280 290 300 NIHVQPRTIY LCRHGENEHN LQGRIGGDSG LSSRGKKFAS ALSKFVEEQN LKDLRVWTSQ 310 320 330 340 350 360 LKSTIQTAEA LRLPYEQWKA LNEIDAGVCE ELTYEEIRDT YPEEYALREQ DKYYYRYPTG 370 380 390 400 410 420 ESYQDLVQRL EPVIMELERQ ENVLVICHQA VLRCLLAYFL DKSAEEMPYL KCPLHTVLKL 430 440 450 460 470 480 TPVAYGCRVE SIYLNVESVC THRERSEDAK KGPNPLMRRN SVTPLASPEP TKKPRINSFE 490 500 510 EHVASTSAAL PSCLPPEVPT QLPGQPLLGQ ACLT
	Isoform 2. Checksum: 32D2924D3D207C17	FASTA	500	57,287
	10 20 30 40 50 60 MPFRKACGPK LTNSPTVIVM VGLPARGKTY ISKKLTRYLN WIGVPTKVFN VGEYRREAVK 70 80 90 100 110 120 QYSSYNFFRP DNEEAMKVRK QCALAALRDV KSYLAKEGGQ IAVFDATNTT RERRHMILHF 130 140 150 160 170 180 AKENDFKAFF IESVCDDPTV VASNIMEVKI SSPDYKDCNS AEAMDDFMKR ISCYEASYQP 190 200 210 220 230 240 LDPDKCDRDL SLIKVIDVGR RFLVNRVQDH IQSRIVYYLM NIHVQPRTIY LCRHGENEHN 250 260 270 280 290 300 LQGRIGGDSG LSSRGKKFAS ALSKFVEEQN LKDLRVWTSQ LKSTIQTAEA LRLPYEQWKA 310 320 330 340 350 360 LNEIDAGVCE ELTYEEIRDT YPEEYALREQ DKYYYRYPTG ESYQDLVQRL EPVIMELERQ 370 380 390 400 410 420 ENVLVICHQA VLRCLLAYFL DKSAEEMPYL KCPLHTVLKL TPVAYGCRVE SIYLNVESVC 430 440 450 460 470 480 THRERSEDAK KGPNPLMRRN SVTPLASPEP TKKPRINSFE EHVASTSAAL PSCLPPEVPT 490 500 QLPGQNMKGS RSSADSSRKH
	Isoform 3. Checksum: 3481133B523AEDB6	FASTA	506	58,021
	10 20 30 40 50 60 MPFRKACGPK LTNSPTVIVM VGLPARGKTY ISKKLTRYLN WIGVPTKVFN VGEYRREAVK 70 80 90 100 110 120 QYSSYNFFRP DNEEAMKVRK QCALAALRDV KSYLAKEGGQ IAVFDATNTT RERRHMILHF 130 140 150 160 170 180 AKENDFKAFF IESVCDDPTV VASNIMEVKI SSPDYKDCNS AEAMDDFMKR ISCYEASYQP 190 200 210 220 230 240 LDPDKCDRDL SLIKVIDVGR RFLVNRVQDH IQSRIVYYLM NIHVQPRTIY LCRHGENEHN 250 260 270 280 290 300 LQGRIGGDSG LSSRGKKFAS ALSKFVEEQN LKDLRVWTSQ LKSTIQTAEA LRLPYEQWKA 310 320 330 340 350 360 LNEIDAGVCE ELTYEEIRDT YPEEYALREQ DKYYYRYPTG ESYQDLVQRL EPVIMELERQ 370 380 390 400 410 420 ENVLVICHQA VLRCLLAYFL DKSAEEMPYL KCPLHTVLKL TPVAYGCRVE SIYLNVESVC 430 440 450 460 470 480 THRERSEDAK KGPNPLMRRN SVTPLASPEP TKKPRINSFE EHVASTSAAL PSCLPPEVPT 490 500 QLPGQPLLGQ ACLRTVCHIF SKFSPY

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Tissue: Brain cortex and Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR859468 mRNA. Translation: CAH91639.1. CR860843 mRNA. Translation: CAH92952.1. CR861456 mRNA. Translation: CAH93512.1.
RefSeq	NP_001125963.1. NP_001128959.1.
UniGene	Pab.18003 Pab.853
3D structure databases
SMR	Q5R9C1. Positions 35-448.
ModBase	Search...
Genome annotation databases
GeneID	100172898. 100189928.
Phylogenomic databases
HOVERGEN	Q5R9C1.
Family and domain databases
InterPro	IPR003094. 6Pfruct_kin. IPR013079. 6Phosfructo_kin. IPR016260. Bifunct_6PFK/fruc_bisP_Ptase. IPR001345. PG/BPGM_mutase. IPR013078. PG_mutase. [Graphical view]
PANTHER	PTHR10606. 6Pfruct_kin. 1 hit.
Pfam	PF01591. 6PF2K. 1 hit. PF00300. PGAM. 1 hit. [Graphical view]
PIRSF	PIRSF000709. 6PFK_2-Ptase. 1 hit.
PRINTS	PR00991. 6PFRUCTKNASE.
PROSITE	PS00175. PG_MUTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F263_PONAB

Accession

Primary (citable) accession number: Q5R9C1
Secondary accession number(s): Q5R404, Q5R5L4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	July 22, 2008
Last modified:	June 16, 2009
This is version 21 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)