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Q5R971

- HEM2_PONAB

UniProt

Q5R971 - HEM2_PONAB

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Protein
Delta-aminolevulinic acid dehydratase
Gene
ALAD
Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Binds 8 zinc ions per octamer. Requires four zinc ions per octamer for full catalytic activity. Can bind up to 2 zinc ions per subunit By similarity.

Enzyme regulationi

Can alternate between a fully active homooctamer and a low-activity homohexamer. A bound magnesium ion may promote the assembly of the fully active homooctamer. The magnesium-binding site is absent in the low-activity homohexamer. Inhibited by compounds that favor the hexameric state. Inhibited by divalent lead ions. The lead ions partially displace the zinc cofactor By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi122 – 1221Zinc 1; catalytic By similarity
Metal bindingi124 – 1241Zinc 1; catalytic By similarity
Metal bindingi131 – 1311Zinc 2 By similarity
Metal bindingi132 – 1321Zinc 1; catalytic By similarity
Active sitei199 – 1991Schiff-base intermediate with substrate By similarity
Binding sitei209 – 2091Substrate 1 By similarity
Binding sitei221 – 2211Substrate 1 By similarity
Metal bindingi223 – 2231Zinc 2 By similarity
Active sitei252 – 2521Schiff-base intermediate with substrate By similarity
Binding sitei279 – 2791Substrate 2 By similarity

GO - Molecular functioni

  1. lead ion binding Source: UniProtKB
  2. porphobilinogen synthase activity Source: UniProtKB
  3. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. heme biosynthetic process Source: UniProtKB
  2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00251; UER00318.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase (EC:4.2.1.24)
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:ALAD
OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Taxonomic identifieri9601 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
ProteomesiUP000001595: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 330330Delta-aminolevulinic acid dehydratase
PRO_0000140529Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei199 – 1991N6-succinyllysine By similarity
Modified residuei215 – 2151Phosphoserine By similarity
Modified residuei252 – 2521N6-succinyllysine By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homooctamer; active form. Homohexamer; low activity form By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ5R971.
SMRiQ5R971. Positions 1-328.

Family & Domainsi

Sequence similaritiesi

Belongs to the ALADH family.

Phylogenomic databases

HOVERGENiHBG001222.
InParanoidiQ5R971.
KOiK01698.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PIRSFiPIRSF001415. Porphbilin_synth. 1 hit.
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5R971-1 [UniParc]FASTAAdd to Basket

« Hide

MQPQSVLHSG YFHPLLRAWQ TATTTLNASN LIYPIFVTDV PDDIQPIASL    50
PGVARYGVNR LEEMLRPLVE EGLRCVLIFG VPSRVPKDER GSAADSEESP 100
AIEAIHLLRK TFPNLLVACD VCLCPYTSHG HCGLLSENGA FQAEESRQRL 150
AEVALAYAKA GCQVVAPSDM MDGRVEAIKE TLMAHGLGSR VSVMSYSAKF 200
ASCFYGPFRD AAKSSPAFGD RRCYQLPPGA RGLALRAVDR DVREGADMLM 250
VKPGMPYLDI VREVKDKHPD LPLAVYHVSG EFAMLWHGAQ AGAFDLKAAV 300
LEAMTAFRRA GADIIITHYT PQLLQWLKEE 330
Length:330
Mass (Da):36,200
Last modified:April 12, 2005 - v2
Checksum:iBFCC5654B32A3FF3
GO

Sequence cautioni

The sequence CAH91689.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR859521 mRNA. Translation: CAH91689.1. Different initiation.
RefSeqiNP_001127135.2. NM_001133663.2.

Genome annotation databases

GeneIDi100174182.
KEGGipon:100174182.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR859521 mRNA. Translation: CAH91689.1 . Different initiation.
RefSeqi NP_001127135.2. NM_001133663.2.

3D structure databases

ProteinModelPortali Q5R971.
SMRi Q5R971. Positions 1-328.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100174182.
KEGGi pon:100174182.

Organism-specific databases

CTDi 210.

Phylogenomic databases

HOVERGENi HBG001222.
InParanoidi Q5R971.
KOi K01698.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00318 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view ]
PANTHERi PTHR11458. PTHR11458. 1 hit.
Pfami PF00490. ALAD. 1 hit.
[Graphical view ]
PIRSFi PIRSF001415. Porphbilin_synth. 1 hit.
PRINTSi PR00144. DALDHYDRTASE.
SMARTi SM01004. ALAD. 1 hit.
[Graphical view ]
PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. The German cDNA consortium
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain cortex.

Entry informationi

Entry nameiHEM2_PONAB
AccessioniPrimary (citable) accession number: Q5R971
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: April 12, 2005
Last modified: March 19, 2014
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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