Q5R8X7 (M3K13_PONAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitogen-activated protein kinase kinase kinase 13
EC=2.7.11.25

Gene names

Name:

MAP3K13

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	966 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Activates the JUN N-terminal pathway through activation of the MAP kinase kinase MAP2K7. Acts synergistically with PRDX3 to regulate the activation of NF-kappa-B in the cytosol. This activation is kinase-dependent and involves activating the IKK complex, the IKBKB-containing complex that phosphorylates inhibitors of NF-kappa-B By similarity. UniProtKB O43283
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity. UniProtKB Q60700
Enzyme regulation	Activated by autophosphorylation and homodimerization By similarity. UniProtKB O43283
Subunit structure	Homodimer; forms dimers through the leucine-zipper motif. Interacts with the C-terminus of MAPK8IP1 through the kinase catalytic domain. Binds PRDX3. Associates with the IKK complex through the kinase domain By similarity. UniProtKB O43283
Subcellular location	Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity.
Post-translational modification	Autophosphorylated on serine and threonine residues By similarity. UniProtKB O43283
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Domain	Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	JNK cascade Inferred from sequence or structural similarity. Source: UniProtKB activation of MAPKK activity Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of NF-kappaB transcription factor activity Inferred from sequence or structural similarity. Source: UniProtKB protein autophosphorylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane fraction Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activity Inferred from sequence or structural similarity. Source: UniProtKB magnesium ion binding Inferred from electronic annotation. Source: InterPro protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 966	966	Mitogen-activated protein kinase kinase kinase 13		PRO_0000086265
Regions
	Domain	168 – 409	242	Protein kinase
	Domain	433 – 454	22	Leucine-zipper 1
	Domain	486 – 507	22	Leucine-zipper 2
	Nucleotide binding	174 – 182	9	ATP By similarity UniProtKB Q12852
	Region	815 – 828	14	Acidic
Sites
	Active site	279	1	Proton acceptor By similarity UniProtKB Q12852
	Binding site	195	1	ATP By similarity UniProtKB Q60700

Sequences

	Sequence		Length	Mass (Da)
	Q5R8X7 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 275CEC4BE01D6A02	FASTA	966	108,442
	10 20 30 40 50 60 MANFQEHLSC SSSPHLPFSE SKTFNGLQDE LTAMGNHPSP KLLEDQQEKG MVRAELIESV 70 80 90 100 110 120 HSPVTTTVLT SVSEDSRDQF ENSVLQLREH DESEMAVSQG NSNTVDAEST SGTEDIKIQF 130 140 150 160 170 180 SRSGSGSGGF LEGLFGCLRP VWNIIGKAYA TDYKLQQQDT WEVPFEEISE LQWLGSGAQG 190 200 210 220 230 240 AVFLGKFRAE EVAIKKVREQ NETDIKHLRK LKHPNIIAFK GVCTQAPCYC IIMEYCAHGQ 250 260 270 280 290 300 LYEVLRAGRK ITPRLLVDWS TGIASGMNYL HLHKIIHRDL KSPNVLVTHT DAVKISDFGT 310 320 330 340 350 360 SKELSDKSTK MSFAGTVAWL APEVIRNEPV SEKVDIWSFG VVLWELLTGE IPYKDVDSSA 370 380 390 400 410 420 IIWGVGSNSL HLPVPSTCPD GFKILMKQTW QSKPRNRPSF RQTLMHLDIA SADVLATPQE 430 440 450 460 470 480 TYFKSQAEWR EEVKKHFEKI KSEGTCIHRL DEELIRRRRE ELRHALDIRE HYERKLERAN 490 500 510 520 530 540 NLYMELSAIM LQLEMREKEL VKREQAVEKK YPGTYKRHPV RPIIHPNAME KLMKRKGVPH 550 560 570 580 590 600 KSGMQTKRPD LLRSEGIPTT EVAPTASPLS GSPKMSTSSS KSRYRSKPRH RRGNSRGSHS 610 620 630 640 650 660 DFVAILKNQP AQENSPNPTY LHQAQSQYPS LHHRNSLQQQ YQQPPPAMSQ SHHPRLNMHG 670 680 690 700 710 720 QDIATCANNL RYFGPAAALR SPLSNHAQRQ LPGSSPDLIS TAMAADCWRG SEPDKDQAGP 730 740 750 760 770 780 WGCCQADPYD PCLQCRPEQY GSLDIPSAEP VGRSPDLSKS PAHNPLLENA QSSEKMEENE 790 800 810 820 830 840 FSSCRSESSL GTSHLVTPPA LPRKTRPLQK SGDDSSEEEE GEVDSEVEFP RRQRPHRCIS 850 860 870 880 890 900 SCQSYSTFSS ENFSVSDGEE GNTSDHSNSP DELADKLEDR LAEKLDDLLS QTPEIPIDIS 910 920 930 940 950 960 SHSDGLSDKE CAVRRVKTQM SLGKLCVEER GYENPMQFEE SDCDSSDGEC SDATVRTNKH YSSATW

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR859621 mRNA. Translation: CAH91783.1.
RefSeq	NP_001126034.1. NM_001132562.1.
UniGene	Pab.1855.
3D structure databases
ProteinModelPortal	Q5R8X7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100172983.
KEGG	pon:100172983.
Organism-specific databases
CTD	9175.
Phylogenomic databases
HOVERGEN	HBG052383.
InParanoid	Q5R8X7.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR017419. MAP3K12_MAP3K13. IPR000719. Prot_kinase_cat_dom. IPR001245. Ser-Thr/Tyr_kinase. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
KO	K04422.
Pfam	PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
PIRSF	PIRSF038165. MAPKKK12_MAPKKK13. 1 hit.
PRINTS	PR00109. TYRKINASE.
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

M3K13_PONAB

Accession

Primary (citable) accession number: Q5R8X7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	December 21, 2004
Last modified:	November 16, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program