Q5R8T5 (SYYC_PONAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine--tRNA ligase, cytoplasmic
EC=6.1.1.1

Alternative name(s):
Tyrosyl-tRNA synthetase
Short name=TyrRS

Gene names

Name:

YARS

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	528 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity.
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Acetylation
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 528	527	Tyrosine--tRNA ligase, cytoplasmic		PRO_0000239690
Regions
	Domain	364 – 468	105	tRNA-binding
	Motif	44 – 52	9	"HIGH" region By similarity
	Motif	222 – 226	5	"KMSKS" region By similarity
Sites
	Binding site	39	1	Tyrosine By similarity
	Binding site	166	1	Tyrosine By similarity
	Binding site	170	1	Tyrosine By similarity
	Binding site	173	1	Tyrosine By similarity
	Binding site	188	1	Tyrosine By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylglycine By similarity
	Modified residue	197	1	N6-acetyllysine By similarity
	Modified residue	206	1	N6-acetyllysine By similarity
	Modified residue	272	1	N6-acetyllysine By similarity
	Modified residue	474	1	N6-acetyllysine By similarity
	Modified residue	482	1	N6-acetyllysine By similarity
	Modified residue	490	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5R8T5 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 67A3AB8DC6755225	FASTA	528	59,167
	10 20 30 40 50 60 MGDAPSPEEK LHLITRNLQE VLGEEKLKEI LKERELKIYW GTATTGKPHV AYFVPMSKIA 70 80 90 100 110 120 DFLKAGCEVT ILFADLHAYL DNMKAPWELL ELRVSYYENV IKAMLESIGV PLEKLTFIKG 130 140 150 160 170 180 TDYQLSKEYT LDVYRLSSVV TQHDSKKAGA EVVKQVEHPL LSGLLYPGLQ ALDEEYLKVD 190 200 210 220 230 240 AQFGGVDQRK IFTFAEKYLP ALGYSKRVHL MNPMVPGLTG SKMSSSEEES KIDLLDRKED 250 260 270 280 290 300 VKKKLKKAFC EPGNVENNGV LSFIKHVLFP LKSEFVILRD ERWGGNKTYT AYMDLEKDFA 310 320 330 340 350 360 AEVVHPGDLK NSVDVALNKL LDPIREKFNT PALKKLASAA YPDPSKQKPM AKGPAKNSEP 370 380 390 400 410 420 EEVIPSRLDI RVGKIITVEK HPDADSLYVE KIDVGEAEPR TVVSGLVQFV PKEELQDRLV 430 440 450 460 470 480 VVLCNLKPQK MRGVESQGML LCASIEGINR QVEPLDPPAG SAPGERVFVK GYEKGQPDEE 490 500 510 520 LKPKKKVFEK LQADFKISEE CIAQWKQTNF MTKLGSISCK SLKGGNIS

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR859664 mRNA. Translation: CAH91825.1.
RefSeq	NP_001126056.1. NM_001132584.1.
UniGene	Pab.18941.
3D structure databases
ProteinModelPortal	Q5R8T5.
SMR	Q5R8T5. Positions 4-342, 360-528.
ModBase	Search...
Proteomic databases
PRIDE	Q5R8T5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100173008.
KEGG	pon:100173008.
Organism-specific databases
CTD	8565.
Phylogenomic databases
HOVERGEN	HBG080113.
InParanoid	Q5R8T5.
Family and domain databases
InterPro	IPR002305. aa-tRNA-synth_Ic. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR014729. Rossmann-like_a/b/a_fold. IPR002547. tRNA-bd_dom. IPR002307. Tyr-tRNA-synth. IPR023617. Tyr-tRNA-synth_arc-type. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01866.
PANTHER	PTHR11946:SF8. Tyr-tRNA_synth. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00234. TyrS. 1 hit.
PROSITE	PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYYC_PONAB

Accession

Primary (citable) accession number: Q5R8T5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 45 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program