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Protein

BUB3-interacting and GLEBS motif-containing protein ZNF207

Gene

ZNF207

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Kinetochore- and microtubule-binding protein that plays a key role in spindle assembly. ZNF207/BuGZ is mainly composed of disordered low-complexity regions and undergoes phase transition or coacervation to form temperature-dependent liquid droplets. Coacervation promotes microtubule bundling and concentrates tubulin, promoting microtubule polymerization and assembly of spindle and spindle matrix by concentrating its building blocks. Also acts as a regulator of mitotic chromosome alignment by mediating the stability and kinetochore loading of BUB3. Mechanisms by which BUB3 is protected are unclear: according to a first report, ZNF207/BuGZ may act by blocking ubiquitination and proteasomal degradation of BUB3. According to another report, the stabilization is independent of the proteasome.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri11 – 3424C2H2-type 1Add
BLAST
Zinc fingeri35 – 5824C2H2-type 2Add
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Mitosis

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
BUB3-interacting and GLEBS motif-containing protein ZNF207By similarity
Short name:
BuGZBy similarity
Alternative name(s):
Zinc finger protein 207By similarity
Gene namesi
Name:ZNF207By similarity
Synonyms:BUGZBy similarity
OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Taxonomic identifieri9601 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
Proteomesi
  • UP000001595 Componenti: Unplaced

Subcellular locationi

  • Nucleus By similarity
  • Chromosomecentromerekinetochore By similarity
  • Cytoplasmcytoskeletonspindle By similarity

  • Note: Localizes primarily to the nucleus in interphase, concentrates at kinetochores prior to nuclear envelope breakdown and during early prometaphase, and disappears from kinetochores upon microtubule-binding.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 494494BUB3-interacting and GLEBS motif-containing protein ZNF207PRO_0000301676Add
BLAST

Interactioni

Subunit structurei

Interacts (via GLEBS region) with BUB3.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9601.ENSPPYP00000009173.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9292Microtubule-binding regionBy similarityAdd
BLAST
Regioni375 – 40733GLEBSBy similarityAdd
BLAST

Domaini

The GLEBS region mediates interaction with BUB3.By similarity
The microtubule-binding region is required for efficient loading of BUB3 onto kinetochores and proper mitosis.By similarity
Mainly composed of disordered low-complexity regions outside of the C2H2-type zinc fingers. Coacervation depends on hydrophobic and aromatic Phe and Tyr in the disordered low-complexity region, that may promote coacervation by forming intermolecular hydrophobic interactions.By similarity

Sequence similaritiesi

Contains 2 C2H2-type zinc fingers.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri11 – 3424C2H2-type 1Add
BLAST
Zinc fingeri35 – 5824C2H2-type 2Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2893. Eukaryota.
ENOG410ZJGQ. LUCA.
HOVERGENiHBG054182.
InParanoidiQ5R8K4.

Family and domain databases

InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5R8K4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRKKKKQLK PWCWYCNRDF DDEKILTQHQ KAKHFKCHIC HKKLYTGPGL
60 70 80 90 100
AIHCMQVHKE TIDAVPNAIP GRTDIELEIY GMEGIPEKDM DEGRRLLEQK
110 120 130 140 150
TQESQKKKQQ DDSDEYDDDD SAASTSFQPQ PVQPQQGYIP PMAQPGLPPV
160 170 180 190 200
PGAPGMPPGI PPLMPGVPPL MPGMPPVMPG MPPGLHHQRK YTQSFCGENI
210 220 230 240 250
MMPMGGMMPP GPGIPPLMPG MPPGMPPPVP RPGIPPMTQA QAVSAPGILN
260 270 280 290 300
RPPAPTATVP APQPPVTKPL FPSAGQMGTP VTSSSTASSN SESLSASSKA
310 320 330 340 350
PFPSTAQAQV AVQGPVGTDF KPLNSTPATS TEPPKPTFPA YTQSTASTTS
360 370 380 390 400
TTNSTAAKPA ASITSKPATL TTTSATSKLI HPDEDISLEE RRAQLPKYQR
410 420 430 440 450
NLPRPGQAPI GNPPVGPIGG MMPPQPGIPQ QQGMRPPMPP HGQYGGHHQG
460 470 480 490
MPGYLPGAMP PYGQGPPMVP PYQGGPPRPP MGMRPPVMSQ GGRY
Length:494
Mass (Da):52,580
Last modified:December 21, 2004 - v1
Checksum:i28EE04241771AE46
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR859748 mRNA. Translation: CAH91906.1.
RefSeqiNP_001126104.1. NM_001132632.1.

Genome annotation databases

GeneIDi100173059.
KEGGipon:100173059.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR859748 mRNA. Translation: CAH91906.1.
RefSeqiNP_001126104.1. NM_001132632.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9601.ENSPPYP00000009173.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100173059.
KEGGipon:100173059.

Organism-specific databases

CTDi7756.

Phylogenomic databases

eggNOGiKOG2893. Eukaryota.
ENOG410ZJGQ. LUCA.
HOVERGENiHBG054182.
InParanoidiQ5R8K4.

Family and domain databases

InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZN207_PONAB
AccessioniPrimary (citable) accession number: Q5R8K4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: December 21, 2004
Last modified: January 20, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.