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Protein

Glucosylceramidase

Gene

GBA

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei274Proton donorBy similarity1
Active sitei379NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Protein family/group databases

CAZyiGH30. Glycoside Hydrolase Family 30.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosylceramidase (EC:3.2.1.45)
Alternative name(s):
Acid beta-glucosidase
Beta-glucocerebrosidase
D-glucosyl-N-acylsphingosine glucohydrolase
Gene namesi
Name:GBA
OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Taxonomic identifieri9601 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
Proteomesi
  • UP000001595 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 39By similarityAdd BLAST39
ChainiPRO_000027864940 – 536GlucosylceramidaseAdd BLAST497

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi43 ↔ 55By similarity
Disulfide bondi57 ↔ 62By similarity
Glycosylationi58N-linked (GlcNAc...)Sequence analysis1
Glycosylationi98N-linked (GlcNAc...)Sequence analysis1
Glycosylationi185N-linked (GlcNAc...)Sequence analysis1
Glycosylationi309N-linked (GlcNAc...)Sequence analysis1
Glycosylationi501N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ5R8E3.

Interactioni

Subunit structurei

Interacts with SCARB2.By similarity

Protein-protein interaction databases

STRINGi9601.ENSPPYP00000000872.

Structurei

3D structure databases

ProteinModelPortaliQ5R8E3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 30 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2566. Eukaryota.
COG5520. LUCA.
HOVERGENiHBG002285.
InParanoidiQ5R8E3.
KOiK01201.
OrthoDBiEOG091G06I4.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR033452. GH30_C.
IPR001139. Glyco_hydro_30.
IPR033453. Glyco_hydro_30_TIM-barrel.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR11069. PTHR11069. 1 hit.
PfamiPF02055. Glyco_hydro_30. 1 hit.
PF17189. Glyco_hydro_30C. 1 hit.
[Graphical view]
PRINTSiPR00843. GLHYDRLASE30.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5R8E3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFSSPSREE CPKPSGRVNI MAGSLTGLLL LQAVSWASGA RPCIPKSFGY
60 70 80 90 100
SSVVCVCNAT YCDSLDPLTF PALGTFSRYE STRSGRRMEL STGTIQANHT
110 120 130 140 150
GTGLLLTLQP EQKFQKVKGF GGAMTDAAAL NILALSPPAQ NLLLKSYFSE
160 170 180 190 200
EGIGYNIIRV PMASCDFSIR TYTYADTPDD FQLHNFSLPE EDTKLKIPLI
210 220 230 240 250
HRALQLARRP VSLLASPWTS PTWLKTNGAV NGKGSLKGQP GDIYHQTWAR
260 270 280 290 300
YFVKFLDAYA EHKLQFWAVT AENEPSAGLL SGYPFQCLGF TPEHQRDFIA
310 320 330 340 350
RDLGPTLANS THHNVRLLML DDQRLLLPHW AKVVLTDPEA AKYVHGIAVH
360 370 380 390 400
WYLDFLAPAK ATLGETHHLF PNTMLFASEA CVGSKFWEQS VRLGSWDRGM
410 420 430 440 450
QYSHSIITNL LYHVVGWTDW NLALNPEGGP NWVRNFVDSP IIVDITKDTF
460 470 480 490 500
YKQPMFYHLG HFSKFIPEGS QRVGLVASQK NDLDTVALMH PDGSAVVVVL
510 520 530
NRSSKDVPLT IKDPAVGFLE TISPGYSIHT YLWRRQ
Length:536
Mass (Da):59,682
Last modified:December 21, 2004 - v1
Checksum:iE4D6A3B89FAFE8CF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61Y → C in CAH90774 (Ref. 1) Curated1
Sequence conflicti316R → H in CAH90774 (Ref. 1) Curated1
Sequence conflicti478S → G in CAH90774 (Ref. 1) Curated1
Sequence conflicti534R → C in CAH90774 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR858547 mRNA. Translation: CAH90774.1.
CR859809 mRNA. Translation: CAH91967.1.
RefSeqiNP_001127488.1. NM_001134016.1.
NP_001128784.1. NM_001135312.1.
UniGeneiPab.19803.

Genome annotation databases

GeneIDi100174563.
100189687.
KEGGipon:100174563.
pon:100189687.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR858547 mRNA. Translation: CAH90774.1.
CR859809 mRNA. Translation: CAH91967.1.
RefSeqiNP_001127488.1. NM_001134016.1.
NP_001128784.1. NM_001135312.1.
UniGeneiPab.19803.

3D structure databases

ProteinModelPortaliQ5R8E3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9601.ENSPPYP00000000872.

Protein family/group databases

CAZyiGH30. Glycoside Hydrolase Family 30.

Proteomic databases

PRIDEiQ5R8E3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100174563.
100189687.
KEGGipon:100174563.
pon:100189687.

Organism-specific databases

CTDi2629.

Phylogenomic databases

eggNOGiKOG2566. Eukaryota.
COG5520. LUCA.
HOVERGENiHBG002285.
InParanoidiQ5R8E3.
KOiK01201.
OrthoDBiEOG091G06I4.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR033452. GH30_C.
IPR001139. Glyco_hydro_30.
IPR033453. Glyco_hydro_30_TIM-barrel.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR11069. PTHR11069. 1 hit.
PfamiPF02055. Glyco_hydro_30. 1 hit.
PF17189. Glyco_hydro_30C. 1 hit.
[Graphical view]
PRINTSiPR00843. GLHYDRLASE30.
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLCM_PONAB
AccessioniPrimary (citable) accession number: Q5R8E3
Secondary accession number(s): Q5RBT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: December 21, 2004
Last modified: October 5, 2016
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.