Reviewed,
UniProtKB/Swiss-Prot Q5R8E3 (GLCM_PONAB)
Last modified
October 13, 2009.
Version 27.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Glucosylceramidase EC=3.2.1.45 Alternative name(s): Beta-glucocerebrosidase Acid beta-glucosidase D-glucosyl-N-acylsphingosine glucohydrolase | ||
| Gene names |
| ||
| Organism | Pongo abelii (Sumatran orangutan) | ||
| Taxonomic identifier | 9601 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo |
Protein attributes
| Sequence length | 536 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Catalytic activity | D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine. |
| Subcellular location | Lysosome membrane; Peripheral membrane protein By similarity. |
| Sequence similarities | Belongs to the glycosyl hydrolase 30 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Lipid metabolism Sphingolipid metabolism |
| Cellular component | Lysosome Membrane |
| Domain | Signal |
| Molecular function | Glycosidase Hydrolase |
| PTM | Disulfide bond Glycoprotein |
| Gene Ontology (GO) | |
| Biological process | carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro lysosome organizationInferred from electronic annotation. Source: InterPro sphingolipid metabolic processInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell lysosomeInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | cation binding Inferred from electronic annotation. Source: InterPro glucosylceramidase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 39 | 39 | By similarity | ||||||||
| Chain | 40 – 536 | 497 | Glucosylceramidase | PRO_0000278649 | |||||||
Sites | |||||||||||
| Active site | 274 | 1 | Proton donor By similarity | ||||||||
| Active site | 379 | 1 | Nucleophile By similarity | ||||||||
Amino acid modifications | |||||||||||
| Glycosylation | 58 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 98 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 185 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 309 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Glycosylation | 501 | 1 | N-linked (GlcNAc...) Potential | ||||||||
| Disulfide bond | 43 ↔ 55 | By similarity | |||||||||
| Disulfide bond | 57 ↔ 62 | By similarity | |||||||||
Experimental info | |||||||||||
| Sequence conflict | 61 | 1 | Y → C in CAH90774. Ref.1 | ||||||||
| Sequence conflict | 316 | 1 | R → H in CAH90774. Ref.1 | ||||||||
| Sequence conflict | 478 | 1 | S → G in CAH90774. Ref.1 | ||||||||
| Sequence conflict | 534 | 1 | R → C in CAH90774. Ref.1 | ||||||||
Sequences
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References
| [1] | The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney. |
Cross-references
Sequence databases | |
|---|---|
| CR858547 mRNA. Translation: CAH90774.1. CR859809 mRNA. Translation: CAH91967.1. | |
| RefSeq | NP_001127488.1. NP_001128784.1. |
| UniGene | Pab.10115 Pab.18904 |
3D structure databases | |
| SMR | Q5R8E3. Positions 40-536. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | GH30. Glycoside Hydrolase Family 30. |
Genome annotation databases | |
| GeneID | 100174563. 100189687. |
Organism-specific databases | |
| CTD | 100174563. |
Phylogenomic databases | |
| HOVERGEN | Q5R8E3. |
Enzyme and pathway databases | |
| BRENDA | 3.2.1.45. 269192. |
Family and domain databases | |
| InterPro | IPR001139. Glyco_hydro_30. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view] |
| Gene3D | G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. |
| PANTHER | PTHR11069. Glyco_hydro_30. 1 hit. |
| Pfam | PF02055. Glyco_hydro_30. 1 hit. [Graphical view] |
| PRINTS | PR00843. GLHYDRLASE30. |
| ProtoNet | Search... |
Entry information
| Entry name | GLCM_PONAB | ||||||||
| Accession | Primary (citable) accession number: Q5R8E3 Secondary accession number(s): Q5RBT6 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


