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Q5R8E3 (GLCM_PONAB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glucosylceramidase
EC=3.2.1.45
Alternative name(s):
Acid beta-glucosidase
Beta-glucocerebrosidase
D-glucosyl-N-acylsphingosine glucohydrolase
Gene names
Name:GBA
OrganismPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) [Reference proteome]
Taxonomic identifier9601 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length536 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

Subunit structure

Interacts with SCARB2 By similarity.

Subcellular location

Lysosome membrane; Peripheral membrane protein By similarity. Note: Targeting to lysosomes occurs through an alternative MPR-independent mechanism via SCARB2 By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 30 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3939 By similarity
Chain40 – 536497Glucosylceramidase
PRO_0000278649

Sites

Active site2741Proton donor By similarity
Active site3791Nucleophile By similarity

Amino acid modifications

Glycosylation581N-linked (GlcNAc...) Potential
Glycosylation981N-linked (GlcNAc...) Potential
Glycosylation1851N-linked (GlcNAc...) Potential
Glycosylation3091N-linked (GlcNAc...) Potential
Glycosylation5011N-linked (GlcNAc...) Potential
Disulfide bond43 ↔ 55 By similarity
Disulfide bond57 ↔ 62 By similarity

Experimental info

Sequence conflict611Y → C in CAH90774. Ref.1
Sequence conflict3161R → H in CAH90774. Ref.1
Sequence conflict4781S → G in CAH90774. Ref.1
Sequence conflict5341R → C in CAH90774. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q5R8E3 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: E4D6A3B89FAFE8CF

FASTA53659,682
        10         20         30         40         50         60 
MEFSSPSREE CPKPSGRVNI MAGSLTGLLL LQAVSWASGA RPCIPKSFGY SSVVCVCNAT 

        70         80         90        100        110        120 
YCDSLDPLTF PALGTFSRYE STRSGRRMEL STGTIQANHT GTGLLLTLQP EQKFQKVKGF 

       130        140        150        160        170        180 
GGAMTDAAAL NILALSPPAQ NLLLKSYFSE EGIGYNIIRV PMASCDFSIR TYTYADTPDD 

       190        200        210        220        230        240 
FQLHNFSLPE EDTKLKIPLI HRALQLARRP VSLLASPWTS PTWLKTNGAV NGKGSLKGQP 

       250        260        270        280        290        300 
GDIYHQTWAR YFVKFLDAYA EHKLQFWAVT AENEPSAGLL SGYPFQCLGF TPEHQRDFIA 

       310        320        330        340        350        360 
RDLGPTLANS THHNVRLLML DDQRLLLPHW AKVVLTDPEA AKYVHGIAVH WYLDFLAPAK 

       370        380        390        400        410        420 
ATLGETHHLF PNTMLFASEA CVGSKFWEQS VRLGSWDRGM QYSHSIITNL LYHVVGWTDW 

       430        440        450        460        470        480 
NLALNPEGGP NWVRNFVDSP IIVDITKDTF YKQPMFYHLG HFSKFIPEGS QRVGLVASQK 

       490        500        510        520        530 
NDLDTVALMH PDGSAVVVVL NRSSKDVPLT IKDPAVGFLE TISPGYSIHT YLWRRQ

« Hide

References

[1]The German cDNA consortium
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CR858547 mRNA. Translation: CAH90774.1.
CR859809 mRNA. Translation: CAH91967.1.
RefSeqNP_001127488.1. NM_001134016.1.
NP_001128784.1. NM_001135312.1.
UniGenePab.18904.
Pab.19803.

3D structure databases

HSSPHSSP built from PDB template 1OGS based on UniProtKB P04062.
ProteinModelPortalQ5R8E3.
SMRQ5R8E3. Positions 40-536.
ModBaseSearch...

Protein family/group databases

CAZyGH30. Glycoside Hydrolase Family 30.

Proteomic databases

PRIDEQ5R8E3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100174563.
100189687.
KEGGpon:100174563.
pon:100189687.

Organism-specific databases

CTD2629.

Phylogenomic databases

HOVERGENHBG002285.
InParanoidQ5R8E3.
KOK01201.

Family and domain databases

Gene3D2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProIPR013780. Glyco_hydro_13_b.
IPR001139. Glyco_hydro_30.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR11069. PTHR11069. 1 hit.
PfamPF02055. Glyco_hydro_30. 1 hit.
[Graphical view]
PRINTSPR00843. GLHYDRLASE30.
SUPFAMSSF51445. Glyco_hydro_cat. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLCM_PONAB
AccessionPrimary (citable) accession number: Q5R8E3
Secondary accession number(s): Q5RBT6
Entry history
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: December 21, 2004
Last modified: May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

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