Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q5R8E3

- GLCM_PONAB

UniProt

Q5R8E3 - GLCM_PONAB

Protein

Glucosylceramidase

Gene

GBA

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (21 Dec 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei274 – 2741Proton donorBy similarity
    Active sitei379 – 3791NucleophileBy similarity

    GO - Molecular functioni

    1. glucosylceramidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. cellular response to tumor necrosis factor Source: Ensembl
    3. ceramide biosynthetic process Source: Ensembl
    4. glucosylceramide catabolic process Source: Ensembl
    5. negative regulation of interleukin-6 production Source: Ensembl
    6. negative regulation of MAP kinase activity Source: Ensembl
    7. positive regulation of protein dephosphorylation Source: Ensembl
    8. sphingosine biosynthetic process Source: Ensembl
    9. termination of signal transduction Source: Ensembl

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Lipid metabolism, Sphingolipid metabolism

    Protein family/group databases

    CAZyiGH30. Glycoside Hydrolase Family 30.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucosylceramidase (EC:3.2.1.45)
    Alternative name(s):
    Acid beta-glucosidase
    Beta-glucocerebrosidase
    D-glucosyl-N-acylsphingosine glucohydrolase
    Gene namesi
    Name:GBA
    OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
    Taxonomic identifieri9601 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
    ProteomesiUP000001595: Unplaced

    Subcellular locationi

    Lysosome membrane By similarity; Peripheral membrane protein By similarity
    Note: Targeting to lysosomes occurs through an alternative MPR-independent mechanism via SCARB2.By similarity

    GO - Cellular componenti

    1. lysosomal lumen Source: Ensembl
    2. lysosomal membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Lysosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3939By similarityAdd
    BLAST
    Chaini40 – 536497GlucosylceramidasePRO_0000278649Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi43 ↔ 55By similarity
    Disulfide bondi57 ↔ 62By similarity
    Glycosylationi58 – 581N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi98 – 981N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi185 – 1851N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi309 – 3091N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi501 – 5011N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ5R8E3.

    Interactioni

    Subunit structurei

    Interacts with SCARB2.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ5R8E3.
    SMRiQ5R8E3. Positions 40-536.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 30 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOVERGENiHBG002285.
    InParanoidiQ5R8E3.
    KOiK01201.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR013780. Glyco_hydro_13_b.
    IPR001139. Glyco_hydro_30.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR11069. PTHR11069. 1 hit.
    PfamiPF02055. Glyco_hydro_30. 1 hit.
    [Graphical view]
    PRINTSiPR00843. GLHYDRLASE30.
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q5R8E3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEFSSPSREE CPKPSGRVNI MAGSLTGLLL LQAVSWASGA RPCIPKSFGY    50
    SSVVCVCNAT YCDSLDPLTF PALGTFSRYE STRSGRRMEL STGTIQANHT 100
    GTGLLLTLQP EQKFQKVKGF GGAMTDAAAL NILALSPPAQ NLLLKSYFSE 150
    EGIGYNIIRV PMASCDFSIR TYTYADTPDD FQLHNFSLPE EDTKLKIPLI 200
    HRALQLARRP VSLLASPWTS PTWLKTNGAV NGKGSLKGQP GDIYHQTWAR 250
    YFVKFLDAYA EHKLQFWAVT AENEPSAGLL SGYPFQCLGF TPEHQRDFIA 300
    RDLGPTLANS THHNVRLLML DDQRLLLPHW AKVVLTDPEA AKYVHGIAVH 350
    WYLDFLAPAK ATLGETHHLF PNTMLFASEA CVGSKFWEQS VRLGSWDRGM 400
    QYSHSIITNL LYHVVGWTDW NLALNPEGGP NWVRNFVDSP IIVDITKDTF 450
    YKQPMFYHLG HFSKFIPEGS QRVGLVASQK NDLDTVALMH PDGSAVVVVL 500
    NRSSKDVPLT IKDPAVGFLE TISPGYSIHT YLWRRQ 536
    Length:536
    Mass (Da):59,682
    Last modified:December 21, 2004 - v1
    Checksum:iE4D6A3B89FAFE8CF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti61 – 611Y → C in CAH90774. 1 PublicationCurated
    Sequence conflicti316 – 3161R → H in CAH90774. 1 PublicationCurated
    Sequence conflicti478 – 4781S → G in CAH90774. 1 PublicationCurated
    Sequence conflicti534 – 5341R → C in CAH90774. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR858547 mRNA. Translation: CAH90774.1.
    CR859809 mRNA. Translation: CAH91967.1.
    RefSeqiNP_001127488.1. NM_001134016.1.
    NP_001128784.1. NM_001135312.1.
    UniGeneiPab.19803.

    Genome annotation databases

    GeneIDi100174563.
    100189687.
    KEGGipon:100174563.
    pon:100189687.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR858547 mRNA. Translation: CAH90774.1 .
    CR859809 mRNA. Translation: CAH91967.1 .
    RefSeqi NP_001127488.1. NM_001134016.1.
    NP_001128784.1. NM_001135312.1.
    UniGenei Pab.19803.

    3D structure databases

    ProteinModelPortali Q5R8E3.
    SMRi Q5R8E3. Positions 40-536.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH30. Glycoside Hydrolase Family 30.

    Proteomic databases

    PRIDEi Q5R8E3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100174563.
    100189687.
    KEGGi pon:100174563.
    pon:100189687.

    Organism-specific databases

    CTDi 2629.

    Phylogenomic databases

    HOVERGENi HBG002285.
    InParanoidi Q5R8E3.
    KOi K01201.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR013780. Glyco_hydro_13_b.
    IPR001139. Glyco_hydro_30.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR11069. PTHR11069. 1 hit.
    Pfami PF02055. Glyco_hydro_30. 1 hit.
    [Graphical view ]
    PRINTSi PR00843. GLHYDRLASE30.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. The German cDNA consortium
      Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.

    Entry informationi

    Entry nameiGLCM_PONAB
    AccessioniPrimary (citable) accession number: Q5R8E3
    Secondary accession number(s): Q5RBT6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 20, 2007
    Last sequence update: December 21, 2004
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3