Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q5R8E3

- GLCM_PONAB

UniProt

Q5R8E3 - GLCM_PONAB

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Glucosylceramidase

Gene

GBA

Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei274 – 2741Proton donorBy similarity
Active sitei379 – 3791NucleophileBy similarity

GO - Molecular functioni

  1. glucosylceramidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. cellular response to tumor necrosis factor Source: Ensembl
  3. ceramide biosynthetic process Source: Ensembl
  4. glucosylceramide catabolic process Source: Ensembl
  5. negative regulation of interleukin-6 production Source: Ensembl
  6. negative regulation of MAP kinase activity Source: Ensembl
  7. positive regulation of protein dephosphorylation Source: Ensembl
  8. sphingosine biosynthetic process Source: Ensembl
  9. termination of signal transduction Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Protein family/group databases

CAZyiGH30. Glycoside Hydrolase Family 30.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosylceramidase (EC:3.2.1.45)
Alternative name(s):
Acid beta-glucosidase
Beta-glucocerebrosidase
D-glucosyl-N-acylsphingosine glucohydrolase
Gene namesi
Name:GBA
OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Taxonomic identifieri9601 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
ProteomesiUP000001595: Unplaced

Subcellular locationi

Lysosome membrane By similarity; Peripheral membrane protein By similarity
Note: Targeting to lysosomes occurs through an alternative MPR-independent mechanism via SCARB2.By similarity

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. lysosomal lumen Source: Ensembl
  3. lysosomal membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3939By similarityAdd
BLAST
Chaini40 – 536497GlucosylceramidasePRO_0000278649Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi43 ↔ 55By similarity
Disulfide bondi57 ↔ 62By similarity
Glycosylationi58 – 581N-linked (GlcNAc...)Sequence Analysis
Glycosylationi98 – 981N-linked (GlcNAc...)Sequence Analysis
Glycosylationi185 – 1851N-linked (GlcNAc...)Sequence Analysis
Glycosylationi309 – 3091N-linked (GlcNAc...)Sequence Analysis
Glycosylationi501 – 5011N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ5R8E3.

Interactioni

Subunit structurei

Interacts with SCARB2.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ5R8E3.
SMRiQ5R8E3. Positions 40-536.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 30 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG002285.
InParanoidiQ5R8E3.
KOiK01201.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR013780. Glyco_hydro_13_b.
IPR001139. Glyco_hydro_30.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR11069. PTHR11069. 1 hit.
PfamiPF02055. Glyco_hydro_30. 1 hit.
[Graphical view]
PRINTSiPR00843. GLHYDRLASE30.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5R8E3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEFSSPSREE CPKPSGRVNI MAGSLTGLLL LQAVSWASGA RPCIPKSFGY
60 70 80 90 100
SSVVCVCNAT YCDSLDPLTF PALGTFSRYE STRSGRRMEL STGTIQANHT
110 120 130 140 150
GTGLLLTLQP EQKFQKVKGF GGAMTDAAAL NILALSPPAQ NLLLKSYFSE
160 170 180 190 200
EGIGYNIIRV PMASCDFSIR TYTYADTPDD FQLHNFSLPE EDTKLKIPLI
210 220 230 240 250
HRALQLARRP VSLLASPWTS PTWLKTNGAV NGKGSLKGQP GDIYHQTWAR
260 270 280 290 300
YFVKFLDAYA EHKLQFWAVT AENEPSAGLL SGYPFQCLGF TPEHQRDFIA
310 320 330 340 350
RDLGPTLANS THHNVRLLML DDQRLLLPHW AKVVLTDPEA AKYVHGIAVH
360 370 380 390 400
WYLDFLAPAK ATLGETHHLF PNTMLFASEA CVGSKFWEQS VRLGSWDRGM
410 420 430 440 450
QYSHSIITNL LYHVVGWTDW NLALNPEGGP NWVRNFVDSP IIVDITKDTF
460 470 480 490 500
YKQPMFYHLG HFSKFIPEGS QRVGLVASQK NDLDTVALMH PDGSAVVVVL
510 520 530
NRSSKDVPLT IKDPAVGFLE TISPGYSIHT YLWRRQ
Length:536
Mass (Da):59,682
Last modified:December 21, 2004 - v1
Checksum:iE4D6A3B89FAFE8CF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti61 – 611Y → C in CAH90774. 1 PublicationCurated
Sequence conflicti316 – 3161R → H in CAH90774. 1 PublicationCurated
Sequence conflicti478 – 4781S → G in CAH90774. 1 PublicationCurated
Sequence conflicti534 – 5341R → C in CAH90774. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR858547 mRNA. Translation: CAH90774.1.
CR859809 mRNA. Translation: CAH91967.1.
RefSeqiNP_001127488.1. NM_001134016.1.
NP_001128784.1. NM_001135312.1.
UniGeneiPab.19803.

Genome annotation databases

GeneIDi100174563.
100189687.
KEGGipon:100174563.
pon:100189687.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR858547 mRNA. Translation: CAH90774.1 .
CR859809 mRNA. Translation: CAH91967.1 .
RefSeqi NP_001127488.1. NM_001134016.1.
NP_001128784.1. NM_001135312.1.
UniGenei Pab.19803.

3D structure databases

ProteinModelPortali Q5R8E3.
SMRi Q5R8E3. Positions 40-536.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH30. Glycoside Hydrolase Family 30.

Proteomic databases

PRIDEi Q5R8E3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100174563.
100189687.
KEGGi pon:100174563.
pon:100189687.

Organism-specific databases

CTDi 2629.

Phylogenomic databases

HOVERGENi HBG002285.
InParanoidi Q5R8E3.
KOi K01201.

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR013780. Glyco_hydro_13_b.
IPR001139. Glyco_hydro_30.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR11069. PTHR11069. 1 hit.
Pfami PF02055. Glyco_hydro_30. 1 hit.
[Graphical view ]
PRINTSi PR00843. GLHYDRLASE30.
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. The German cDNA consortium
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiGLCM_PONAB
AccessioniPrimary (citable) accession number: Q5R8E3
Secondary accession number(s): Q5RBT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: December 21, 2004
Last modified: October 29, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3