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Q5R8A5

- PA24A_PONAB

UniProt

Q5R8A5 - PA24A_PONAB

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Protein
Cytosolic phospholipase A2
Gene
PLA2G4A, CPLA2, PLA2G4
Organism
Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response By similarity.

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Stimulated by agonists such as ATP, EGF, thrombin and bradykinin as well as by cytosolic Ca2+ By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi40 – 401Calcium 1 By similarity
Metal bindingi40 – 401Calcium 2 By similarity
Metal bindingi41 – 411Calcium 1; via carbonyl oxygen By similarity
Metal bindingi43 – 431Calcium 1 By similarity
Metal bindingi43 – 431Calcium 2 By similarity
Metal bindingi65 – 651Calcium 1 By similarity
Metal bindingi93 – 931Calcium 2 By similarity
Metal bindingi94 – 941Calcium 2; via carbonyl oxygen By similarity
Metal bindingi95 – 951Calcium 2 By similarity
Active sitei228 – 2281Nucleophile By similarity
Active sitei549 – 5491Proton acceptor By similarity

GO - Molecular functioni

  1. calcium ion binding Source: UniProtKB
  2. calcium-dependent phospholipid binding Source: UniProtKB
  3. lysophospholipase activity Source: UniProtKB-EC
  4. phospholipase A2 activity Source: UniProtKB-EC

GO - Biological processi

  1. phospholipid catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic phospholipase A2
Short name:
cPLA2
Alternative name(s):
Phospholipase A2 group IVA
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Alternative name(s):
Phosphatidylcholine 2-acylhydrolase
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:PLA2G4A
Synonyms:CPLA2, PLA2G4
OrganismiPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii)
Taxonomic identifieri9601 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo
ProteomesiUP000001595: Unplaced

Subcellular locationi

Cytoplasm By similarity. Cytoplasmic vesicle By similarity
Note: Translocates to membrane vesicles in a calcium-dependent fashion By similarity.

GO - Cellular componenti

  1. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 749749Cytosolic phospholipase A2
PRO_0000345134Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei268 – 2681Phosphothreonine By similarity
Modified residuei437 – 4371Phosphoserine By similarity
Modified residuei505 – 5051Phosphoserine; by MAPK By similarity
Modified residuei727 – 7271Phosphoserine By similarity
Modified residuei729 – 7291Phosphoserine By similarity

Post-translational modificationi

Activated by phosphorylation at both Ser-505 and Ser-727 By similarity.

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Interacts with KAT5 By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ5R8A5.
SMRiQ5R8A5. Positions 13-721.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 106102C2
Add
BLAST
Domaini140 – 740601PLA2c
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 178178Phospholipid binding By similarity
Add
BLAST

Domaini

The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca2+ By similarity.

Sequence similaritiesi

Contains 1 C2 domain.
Contains 1 PLA2c domain.

Phylogenomic databases

HOGENOMiHOG000115420.
HOVERGENiHBG053479.
InParanoidiQ5R8A5.
KOiK16342.

Family and domain databases

Gene3Di2.60.40.150. 1 hit.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEiPS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5R8A5-1 [UniParc]FASTAAdd to Basket

« Hide

MSFIDPYQHI IVEHQYSHKF TVVVLCATKV TKGAFGDMLD TPDPYVELFI    50
STTPDSRKRT RHFNNDINPV WNETFEFILD PNQENVLEIT LMDANYVMDE 100
TLGTATFPVS SMKVGEKKEV PFIFNQVTEM ILEMSLEVCS CPDLRFSMAL 150
CDQEKTFRQQ RKEHIRESMK KLLGPKNSEG LHSARDVPVV AILGSGGGFR 200
AMVGFSGVMK ALYESGILDC ATYVAGLSGS TWYMSTLYSH PDFPEKGPEE 250
INEELMKNVS HNPLLLLTPQ KVKRYVESLW KKKSSGQPVT FTDIFGMLIG 300
ETLIHNRMNT TLSSLKEKVN TAQCPLPLFT CLHVKPDVSE LMFADWVEFS 350
PYEIGMAKYG TFMAPDLFGS KFFMGTVVKK YEENPLHFLM GVWGSAFSIL 400
FNRVLGVSGS QSRGSTMEEE LENITTKHIV SNDSSDSDDE SHEPKGTENE 450
DAGSDYQSDN QASWIHRMIM ALVSDSALFN TREGRAGKVH NFMLGLNLNT 500
SYPLSPLSDF ATQDSFDDDE LDAAVADPDE FERIYEPLDV KSKKIHVVDS 550
GLTFNLPYPL ILRPQRGVDL IISFDFSARP SDSSPPFKEL LLAEKWAKMN 600
KLPFPKIDPY VFDREGLKEC YVFKPKNPDM EKDCPTIIHF VLANINFRKY 650
KAPGVPRETE EEKEIADFDI FDDPESPFST FNFQYPNQAF KRLHDLMHFN 700
TLNNIDVIKE AMVESIEYRR QNPSRCSVSL SNVEARRFFN KEFLSKPKA 749
Length:749
Mass (Da):85,168
Last modified:December 21, 2004 - v1
Checksum:i70C1D0C9E983BBD4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR859848 mRNA. Translation: CAH92005.1.
RefSeqiNP_001126164.1. NM_001132692.1.
UniGeneiPab.12757.

Genome annotation databases

GeneIDi100173125.
KEGGipon:100173125.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR859848 mRNA. Translation: CAH92005.1 .
RefSeqi NP_001126164.1. NM_001132692.1.
UniGenei Pab.12757.

3D structure databases

ProteinModelPortali Q5R8A5.
SMRi Q5R8A5. Positions 13-721.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100173125.
KEGGi pon:100173125.

Organism-specific databases

CTDi 5321.

Phylogenomic databases

HOGENOMi HOG000115420.
HOVERGENi HBG053479.
InParanoidi Q5R8A5.
KOi K16342.

Family and domain databases

Gene3Di 2.60.40.150. 1 hit.
InterProi IPR016035. Acyl_Trfase/lysoPLipase.
IPR000008. C2_dom.
IPR002642. LysoPLipase_cat_dom.
[Graphical view ]
Pfami PF00168. C2. 1 hit.
PF01735. PLA2_B. 1 hit.
[Graphical view ]
SMARTi SM00239. C2. 1 hit.
SM00022. PLAc. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF52151. SSF52151. 1 hit.
PROSITEi PS50004. C2. 1 hit.
PS51210. PLA2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. The German cDNA consortium
    Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiPA24A_PONAB
AccessioniPrimary (citable) accession number: Q5R8A5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: December 21, 2004
Last modified: April 16, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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