Q5R806 (PTGR2_PONAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prostaglandin reductase 2
Short name=PRG-2
EC=1.3.1.48

Alternative name(s):
15-oxoprostaglandin 13-reductase

Gene names

Name:

PTGR2

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest activity towards 15-keto-PGE2. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation By similarity.
Catalytic activity	11-alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+ = (5Z)-(13E)-11-alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NADP-dependent oxidoreductase L4BD family.
Sequence caution	The sequence CAH92104.1 differs from that shown. Reason: Erroneous termination at position 352. Translated as stop.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	prostaglandin metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	15-oxoprostaglandin 13-oxidase activity Inferred from sequence or structural similarity. Source: UniProtKB nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Prostaglandin reductase 2		PRO_0000218072
Regions
	Nucleotide binding	165 – 168	4	NADP By similarity
	Nucleotide binding	253 – 259	7	NADP By similarity
	Nucleotide binding	287 – 289	3	NADP By similarity
	Region	99 – 100	2	Substrate binding By similarity
	Region	288 – 290	3	Substrate binding By similarity
Sites
	Binding site	192	1	NADP By similarity
	Binding site	208	1	NADP By similarity
	Binding site	231	1	NADP By similarity
	Binding site	337	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5R806 [UniParc]. Last modified April 26, 2005. Version 2. Checksum: A0BF58632E0CE7D8	FASTA	351	38,479
	10 20 30 40 50 60 MIVQRVVLNS RPGKNGNPVA ENFRMEEVYL PDNINEGQVQ VRTLYLSVDP YMRCRMNEDT 70 80 90 100 110 120 GTDYITPWQL SQVVDGGGIG IIEESKHTNL TKGDFVTSFY WPWQTKVILD GNSLEKVDPQ 130 140 150 160 170 180 LVDGHLSYFL GAIGMPGLTS LIGIQEKGHI TAGSNKTMVV SGAAGACGSV AGQIGHLLGC 190 200 210 220 230 240 SRVVGICGTH EKCVLLTSEL GFDAAINYKK DNVAEQLRES CPAGVDVYFD NVGGNISDTV 250 260 270 280 290 300 ISQMNENSHI ILCGQISQYN KDVPYPPPLS PAIEAIQKER NITRERFLVL NYRDKFEPGI 310 320 330 340 350 LQLSQWFKEG KLKIKETVIN GLENMGAAFQ SMMTGGNIGK QIVCISEEIS L

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR859950 mRNA. Translation: CAH92104.1. Sequence problems.
UniGene	Pab.5681.
3D structure databases
ProteinModelPortal	Q5R806.
SMR	Q5R806. Positions 1-351.
ModBase	Search...
Proteomic databases
PRIDE	Q5R806.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG055024.
InParanoid	Q5R806.
Family and domain databases
InterPro	IPR013149. ADH_C. IPR002085. ADH_SF_Zn-type. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 2 hits.
ProtoNet	Search...

Entry information

Entry name

PTGR2_PONAB

Accession

Primary (citable) accession number: Q5R806

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	April 26, 2005
Last modified:	December 14, 2011
This is version 41 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program