Q5R7X9 (RAD1_PONAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cell cycle checkpoint protein RAD1
EC=3.1.11.2

Alternative name(s):
DNA repair exonuclease rad1 homolog

Gene names

Name:

RAD1

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHINO to sites of double-stranded breaks (DSB) occurring during the S phase. Possesses 3'->5' double stranded DNA exonuclease activity By similarity.
Catalytic activity	Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.
Subunit structure	Component of the toroidal 9-1-1 (RAD9-RAD1-HUS1) complex, composed of RAD9A, RAD1 and HUS1. The 9-1-1 complex associates with LIG1, POLB, FEN1, RAD17, HDAC1, RPA1 and RPA2. The 9-1-1 complex associates with the RAD17-RFC complex. RAD1 interacts with POLB, FEN1, HUS1, HUS1B, RAD9A and RAD9B. Interacts with DNAJC7 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the rad1 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Molecular function	Exonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	damaged DNA binding Inferred from electronic annotation. Source: InterPro exodeoxyribonuclease III activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	Cell cycle checkpoint protein RAD1		PRO_0000225007

Sequences

	Sequence		Length	Mass (Da)
	Q5R7X9 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 075FBD4CF8A4FDB2	FASTA	282	31,827
	10 20 30 40 50 60 MPLLTQQIQD EDDQYSLVAS LDNVRNLSTI LKAIHFREHA TCFATKNGIK VTVENAKCVQ 70 80 90 100 110 120 ANAFIQAGIF QEFKVQEESV TFRINLTVLL DCLSIFGSSP MPGTLTALRM CYQGYGYPLM 130 140 150 160 170 180 LFLEEGGVVT VCKINTQEPE ETLDFDFCST NVINKIILQS EGLREAFSEL DMTSEVLQIT 190 200 210 220 230 240 MSPDKPYFRL STFGNAGSSH LDYPKDSDLM EAFHCNQTQV NRYKISLLKP STKALVLSCK 250 260 270 280 VSIRTDNRGF LSLQYMIRNE DGQICFVEYY CCPDEEVPES ES

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR859979 mRNA. Translation: CAH92131.1.
RefSeq	NP_001126246.1. NM_001132774.1.
UniGene	Pab.2612.
3D structure databases
ProteinModelPortal	Q5R7X9.
SMR	Q5R7X9. Positions 13-275.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSPPYT00000017888; ENSPPYP00000017189; ENSPPYG00000015389.
GeneID	100173217.
KEGG	pon:100173217.
Organism-specific databases
CTD	5810.
Phylogenomic databases
HOVERGEN	HBG053058.
InParanoid	Q5R7X9.
OMA	KAIHFRD.
Family and domain databases
InterPro	IPR003021. Rad1_Rec1. IPR003011. Rad1_repair. [Graphical view]
KO	K02830.
PANTHER	PTHR10870. Rad1_Rec1. 1 hit.
Pfam	PF02144. Rad1. 1 hit. [Graphical view]
PRINTS	PR01245. RAD1REC1. PR01246. RAD1REPAIR.
ProtoNet	Search...

Entry information

Entry name

RAD1_PONAB

Accession

Primary (citable) accession number: Q5R7X9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	December 21, 2004
Last modified:	November 16, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program