Q5R7N2 (TWF1_PONAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Twinfilin-1

Gene names

Name:

TWF1

Organism

Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) [Reference proteome]

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Ponginae › Pongo

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles By similarity.
Subunit structure	Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF2 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down-regulated By similarity.
Subcellular location	Cytoplasm. Cytoplasm › cytoskeleton By similarity. Note: Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts By similarity.
Post-translational modification	Phosphorylated on serine and threonine residues By similarity.
Sequence similarities	Belongs to the actin-binding proteins ADF family. Twinfilin subfamily. Contains 2 ADF-H domains.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Domain	Repeat
Ligand	Actin-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 350	349	Twinfilin-1		PRO_0000232405
Regions
	Domain	2 – 139	138	ADF-H 1
	Domain	175 – 313	139	ADF-H 2
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	143	1	Phosphoserine By similarity
	Modified residue	309	1	Phosphotyrosine By similarity
	Modified residue	349	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5R7N2 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 47C2FEBD973D8770	FASTA	350	40,195
	10 20 30 40 50 60 MSHRTGIQAS EDVKEIFARA RNGKYRLLKI SIENEQLVIG SYSQPSDSWD KDYDSFVLPL 70 80 90 100 110 120 LEDKQPCYIL FRLDSQNAQG YEWIFIAWSP DHSHVRQKML YAATRATLKK EFGGGHIKDE 130 140 150 160 170 180 VFGTVKEDVS LHGYKKYLLS QSSPAPLTAA EEELRQIKIN EVQTDVGVDT KHQTLQGVAF 190 200 210 220 230 240 PISREAFQAL EKLNNRQLNY VQLEIDIKNE IIILANTTDT ELKDLPKRIP KDSARYHFFL 250 260 270 280 290 300 YKHSHEGDYL ESIVFIYSMP GYTCSIRERM LYSSCKSPLL EIVERQLQMG VIRKIEIDNG 310 320 330 340 350 DELTADFLYE EVHPKQHAHK QSFAKPKGPA GKRGIRRLIR GPAETEATTD

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Web resources

Protein Spotlight

Molecular embrace - Issue 73 of August 2006

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR860082 mRNA. Translation: CAH92228.1.
RefSeq	NP_001126303.1. NM_001132831.1.
UniGene	Pab.18838.
3D structure databases
ProteinModelPortal	Q5R7N2.
SMR	Q5R7N2. Positions 7-139, 161-313.
ModBase	Search...
Proteomic databases
PRIDE	Q5R7N2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100173282.
KEGG	pon:100173282.
Organism-specific databases
CTD	5756.
Phylogenomic databases
HOVERGEN	HBG000848.
InParanoid	Q5R7N2.
KO	K08870.
Family and domain databases
InterPro	IPR002108. Actin-bd_cofilin/tropomyosin. [Graphical view]
Pfam	PF00241. Cofilin_ADF. 2 hits. [Graphical view]
SMART	SM00102. ADF. 2 hits. [Graphical view]
PROSITE	PS51263. ADF_H. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TWF1_PONAB

Accession

Primary (citable) accession number: Q5R7N2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	December 21, 2004
Last modified:	November 28, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program