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Q5R7N2 (TWF1_PONAB) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein attributes

Sequence length350 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles By similarity.

Subunit structure

Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF2 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down-regulated By similarity.

Subcellular location

Cytoplasm. Cytoplasmcytoskeleton By similarity. Note: Diffuse cytoplasmic localization with perinuclear and G-actin-rich cortical actin structures sublocalization. Also found at membrane ruffles and cell-cell contacts By similarity.

Post-translational modification

Phosphorylated on serine and threonine residues By similarity.

Sequence similarities

Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.

Contains 2 ADF-H domains.

Ontologies

Keywords
   Cellular componentCytoplasm
Cytoskeleton
   DomainRepeat
   LigandActin-binding
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processnegative regulation of actin filament polymerization

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoskeleton

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 350349Twinfilin-1
PRO_0000232405

Regions

Domain2 – 139138ADF-H 1
Domain175 – 313139ADF-H 2

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue1431Phosphoserine By similarity
Modified residue3091Phosphotyrosine By similarity
Modified residue3491Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5R7N2 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: 47C2FEBD973D8770

FASTA35040,195
        10         20         30         40         50         60 
MSHRTGIQAS EDVKEIFARA RNGKYRLLKI SIENEQLVIG SYSQPSDSWD KDYDSFVLPL 

        70         80         90        100        110        120 
LEDKQPCYIL FRLDSQNAQG YEWIFIAWSP DHSHVRQKML YAATRATLKK EFGGGHIKDE 

       130        140        150        160        170        180 
VFGTVKEDVS LHGYKKYLLS QSSPAPLTAA EEELRQIKIN EVQTDVGVDT KHQTLQGVAF 

       190        200        210        220        230        240 
PISREAFQAL EKLNNRQLNY VQLEIDIKNE IIILANTTDT ELKDLPKRIP KDSARYHFFL 

       250        260        270        280        290        300 
YKHSHEGDYL ESIVFIYSMP GYTCSIRERM LYSSCKSPLL EIVERQLQMG VIRKIEIDNG 

       310        320        330        340        350 
DELTADFLYE EVHPKQHAHK QSFAKPKGPA GKRGIRRLIR GPAETEATTD 

« Hide

References

[1]The German cDNA consortium
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.

Web resources

Protein Spotlight

Molecular embrace - Issue 73 of August 2006

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR860082 mRNA. Translation: CAH92228.1.
RefSeqNP_001126303.1. NM_001132831.1.

3D structure databases

ProteinModelPortalQ5R7N2.
SMRQ5R7N2. Positions 7-139, 161-313.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ5R7N2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100173282.
KEGGpon:100173282.

Organism-specific databases

CTD5756.

Phylogenomic databases

HOVERGENHBG000848.
InParanoidQ5R7N2.
KOK08870.

Family and domain databases

Gene3D3.40.20.10. 2 hits.
InterProIPR002108. ADF-H.
IPR029006. ADF-H/Gelsolin-like_dom.
IPR028458. Twinfilin.
[Graphical view]
PANTHERPTHR13759. PTHR13759. 1 hit.
PfamPF00241. Cofilin_ADF. 2 hits.
[Graphical view]
SMARTSM00102. ADF. 2 hits.
[Graphical view]
PROSITEPS51263. ADF_H. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTWF1_PONAB
AccessionPrimary (citable) accession number: Q5R7N2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: December 21, 2004
Last modified: June 11, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries