Q5R7C9 (AK1C3_PONAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aldo-keto reductase family 1 member C3 homolog
EC=1.-.-.-

Alternative name(s):
17-beta-hydroxysteroid dehydrogenase type 5
Short name=17-beta-HSD 5
3-alpha-hydroxysteroid dehydrogenase type 2
Short name=3-alpha-HSD type 2
EC=1.1.1.213
Indanol dehydrogenase
EC=1.1.1.112
Prostaglandin F synthase
Short name=PGFS
EC=1.1.1.188
Testosterone 17-beta-dehydrogenase 5
EC=1.1.1.239
EC=1.1.1.64
Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase
EC=1.3.1.20

Gene names

Name:

AKR1C3

Organism

Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) [Reference proteome]

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Ponginae › Pongo

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta-PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone By similarity.
Catalytic activity	Trans-1,2-dihydrobenzene-1,2-diol + NADP+ = catechol + NADPH. A 3-alpha-hydroxysteroid + NAD(P)+ = a 3-oxosteroid + NAD(P)H. (5Z,13E)-(15S)-9-alpha,11-alpha,15-trihydroxyprosta-5,13-dienoate + NADP+ = (5Z,13E)-(15S)-9-alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH. Testosterone + NAD+ = androstenedione + NADH. Testosterone + NADP+ = androst-4-ene-3,17-dione + NADPH. Indan-1-ol + NAD(P)+ = indanone + NAD(P)H.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to cadmium ion Inferred from sequence or structural similarity. Source: UniProtKB cellular response to calcium ion Inferred from sequence or structural similarity. Source: UniProtKB cellular response to corticosteroid stimulus Inferred from sequence or structural similarity. Source: UniProtKB cellular response to jasmonic acid stimulus Inferred from sequence or structural similarity. Source: UniProtKB cellular response to prostaglandin stimulus Inferred from sequence or structural similarity. Source: UniProtKB cellular response to reactive oxygen species Inferred from sequence or structural similarity. Source: UniProtKB daunorubicin metabolic process Inferred from sequence or structural similarity. Source: UniProtKB farnesol catabolic process Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of retinoic acid biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of endothelial cell apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of protein kinase B signaling cascade Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of reactive oxygen species metabolic process Inferred from sequence or structural similarity. Source: UniProtKB progesterone metabolic process Inferred from sequence or structural similarity. Source: UniProtKB prostaglandin metabolic process Inferred from electronic annotation. Source: GOC protein import into nucleus, translocation Inferred from sequence or structural similarity. Source: UniProtKB regulation of retinoic acid receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB regulation of testosterone biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB retinal metabolic process Inferred from sequence or structural similarity. Source: UniProtKB testosterone biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	15-hydroxyprostaglandin-D dehydrogenase (NADP+) activity Inferred from sequence or structural similarity. Source: UniProtKB alditol:NADP+ 1-oxidoreductase activity Inferred from sequence or structural similarity. Source: UniProtKB androsterone dehydrogenase (A-specific) activity Inferred from electronic annotation. Source: EC androsterone dehydrogenase activity Inferred from sequence or structural similarity. Source: UniProtKB delta4-3-oxosteroid 5beta-reductase activity Inferred from sequence or structural similarity. Source: UniProtKB dihydrotestosterone 17-beta-dehydrogenase activity Inferred from sequence or structural similarity. Source: UniProtKB geranylgeranyl reductase activity Inferred from sequence or structural similarity. Source: UniProtKB indanol dehydrogenase activity Inferred from electronic annotation. Source: EC ketoreductase activity Inferred from sequence or structural similarity. Source: UniProtKB ketosteroid monooxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor Inferred from sequence or structural similarity. Source: UniProtKB phenanthrene 9,10-monooxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB prostaglandin D2 11-ketoreductase activity Inferred from electronic annotation. Source: EC prostaglandin F receptor activity Inferred from sequence or structural similarity. Source: UniProtKB prostaglandin-F synthase activity Inferred from electronic annotation. Source: EC retinal dehydrogenase activity Inferred from sequence or structural similarity. Source: UniProtKB retinol dehydrogenase activity Inferred from sequence or structural similarity. Source: UniProtKB testosterone 17-beta-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC testosterone dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	Aldo-keto reductase family 1 member C3 homolog		PRO_0000124639
Regions
	Nucleotide binding	13 – 22	10	NADP Potential
	Nucleotide binding	217 – 280	64	NADP By similarity
Sites
	Active site	55	1	Proton donor By similarity
	Binding site	117	1	Substrate By similarity
	Site	54	1	Important for substrate specificity By similarity
	Site	84	1	Lowers pKa of active site Tyr By similarity
	Site	227	1	Involved in ligand recognition and product release By similarity
	Site	306	1	Involved in ligand recognition and product release By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5R7C9 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: DCAD6ECA97C204AB	FASTA	323	36,848
	10 20 30 40 50 60 MDSKHQCVKL NDGHFMPVLG FGTYAPPEVP RSKALEVTKL AIEAGFRHID SAHLYNNEEQ 70 80 90 100 110 120 VGLAIRSKIA DGSVKREDIF YTSKLWSTFH RPELVRPALE NSLKKAQLDY VDLYLIHSPM 130 140 150 160 170 180 SLKPGEELSP TDENGKVIFD IVDLCTTWEA MEECKDAGLA KSIGVSNFNR RQLEMILNKP 190 200 210 220 230 240 GLKYKPVCNQ VECHPYFNRS KLLDFCKSKD IVLVAYSALG SQRDKRWVDP NSPVLLEDPV 250 260 270 280 290 300 LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIREN VQVFEFQLTA EDMRAIDGLN 310 320 RNLHYFNSDS LASHPNYPYS DEY

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR860189 mRNA. Translation: CAH92331.1.
RefSeq	NP_001127540.1. NM_001134068.1.
UniGene	Pab.18808.
3D structure databases
ProteinModelPortal	Q5R7C9.
SMR	Q5R7C9. Positions 6-321.
ModBase	Search...
Proteomic databases
PRIDE	Q5R7C9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100174617.
KEGG	pon:100174617.
Organism-specific databases
CTD	8644.
Phylogenomic databases
HOVERGEN	HBG000020.
InParanoid	Q5R7C9.
Family and domain databases
Gene3D	3.20.20.100. 1 hit.
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. IPR020471. Aldo/keto_reductase_subgr. IPR023210. NADP_OxRdtase_dom. [Graphical view]
PANTHER	PTHR11732. PTHR11732. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
PIRSF	PIRSF000097. AKR. 1 hit.
PRINTS	PR00069. ALDKETRDTASE.
SUPFAM	SSF51430. Aldo/ket_red. 1 hit.
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AK1C3_PONAB

Accession

Primary (citable) accession number: Q5R7C9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	December 21, 2004
Last modified:	April 3, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program