Reviewed, UniProtKB/Swiss-Prot Q5R7B5 (KCRS_PONAB)

Names and origin

Protein names

Recommended name:
    Creatine kinase, sarcomeric mitochondrial
    EC=2.7.3.2
Alternative name(s):
    S-MtCK

Gene names

Name:

CKMT2

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa By similarity.
Catalytic activity	ATP + creatine = ADP + phosphocreatine.
Subunit structure	Exists as an octamer composed of four CKMT2 homodimers By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane sideBy similarity.
Miscellaneous	Mitochondrial creatine kinase binds cardiolipin.
Sequence similarities	Belongs to the ATP:guanido phosphotransferase family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW creatine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 39	39	Mitochondrion
	Chain	40 – 419	380	Creatine kinase, sarcomeric mitochondrial		PRO_0000016596
Regions
	Nucleotide binding	162 – 166	5	ATP By similarity
	Nucleotide binding	354 – 359	6	ATP By similarity
	Region	40 – 64	25	Cardiolipin-binding By similarity
Sites
	Binding site	225	1	ATP By similarity
	Binding site	270	1	ATP By similarity
	Binding site	326	1	ATP By similarity
	Binding site	369	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5R7B5-1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: E10C42A26BD926FE	FASTA	419	47,476
	10 20 30 40 50 60 MASIFSKLLT GRNASLLFAT MGTSVLTTGY LLNQQKVCAE VREQPRLFPP SADYPDLRKH 70 80 90 100 110 120 NNCMAECLTP AIYAKLRNKV TPNGYTLDQC IQTGVDNPGH PFIKTVGMVA GDEESYEVFA 130 140 150 160 170 180 DLFDPVIKLR HNGYDPRVMK HTTDLDASKI TQGQFDEHYV LSSRVRTGRS IRGLSLPPAC 190 200 210 220 230 240 TRAERREVEN VAITALEGLK GDLAGRYYKL SEMTEQDQQR LIDDHFLFDK PVSPLLTCAG 250 260 270 280 290 300 MARDWPDARG IWHNYDKTFL IWINEEDHTR VISMEKGGNM KRVFERFCRG LKEVERLIQE 310 320 330 340 350 360 RGWEFMWNER LGYILTCPSN LGTGLRAGVH VRIPKLSKDP RFSKILENLR LQKRGTGGVD 370 380 390 400 410 TAAVADVYDI SNIDRIGRSE VELVQIVIDG VNYLVDCEKK LERGQDIKVP PPLPQFGKK

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR860203 mRNA. Translation: CAH92345.1.
RefSeq	NP_001126380.1.
3D structure databases
SMR	Q5R7B5. Positions 47-413.
ModBase	Search...
Genome annotation databases
GeneID	100173361.
Phylogenomic databases
HOVERGEN	Q5R7B5.
Family and domain databases
InterPro	IPR000749. ATP-gua_Ptrans. IPR014746. Gln_synth/guanido_kin_cat. [Graphical view]
Gene3D	G3DSA:1.10.135.10. ATP-gua_Ptrans. 1 hit. G3DSA:3.30.590.10. ATP-gua_Ptrans. 1 hit.
PANTHER	PTHR11547. ATP-gua_Ptrans. 1 hit.
Pfam	PF00217. ATP-gua_Ptrans. 1 hit. PF02807. ATP-gua_PtransN. 1 hit. [Graphical view]
PROSITE	PS00112. GUANIDO_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KCRS_PONAB

Accession

Primary (citable) accession number: Q5R7B5

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	December 21, 2004
Last modified:	November 4, 2008
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)