Reviewed, UniProtKB/Swiss-Prot Q5R7A9 (LYAG_PONAB)

Names and origin

Protein names

Recommended name:
    Lysosomal alpha-glucosidase
    EC=3.2.1.20
Alternative name(s):
    Acid maltase

Gene names

Name:

GAA

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	952 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Essential for the degradation of glygogen to glucose in lysosomes By similarity.
Catalytic activity	Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.
Subcellular location	Lysosome By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 31 family. Contains 1 P-type (trefoil) domain.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Propeptide	28 – 69	42	By similarity		PRO_0000260440
	Chain	70 – 952	883	Lysosomal alpha-glucosidase		PRO_0000260441
Regions
	Domain	80 – 131	52	P-type
Sites
	Active site	518	1	Nucleophile By similarity
	Active site	521	1	By similarity
Amino acid modifications
	Glycosylation	140	1	N-linked (GlcNAc...) Potential
	Glycosylation	233	1	N-linked (GlcNAc...) Potential
	Glycosylation	390	1	N-linked (GlcNAc...) Potential
	Glycosylation	470	1	N-linked (GlcNAc...) Potential
	Glycosylation	652	1	N-linked (GlcNAc...) Potential
	Glycosylation	882	1	N-linked (GlcNAc...) Potential
	Glycosylation	925	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5R7A9-1 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 4AEC592583525C16	FASTA	952	105,531
	10 20 30 40 50 60 MRVRHPPCSR RLLAICALVS LATAALLGHI LLHDFLLVPR ELSGSSPVLE ETHPAHQQGA 70 80 90 100 110 120 SRPGPRDAQA HLGRPRAVPT QCDVPPNSRF DCAPDKAITR EQCDARGCCY IPAKQGLRGA 130 140 150 160 170 180 QMGQPWCFFP PSYPSYKLEN LSSSEMGYTA TLTRTTPTFF PKDILTLRLD VMMETENRLH 190 200 210 220 230 240 FTIKDPANRR YEVPLETPRV HSRAPSPLYS VEFSEEPFGV IVRRQLDGRV LLNTTVAPLF 250 260 270 280 290 300 FADQFLQLST SLPSQYITGL AEHLSPLMLS TSWTRVTLWN RDLAPTPGAN LYGSHPFYLA 310 320 330 340 350 360 LEDGGSAHGV FLLNSNAMDV VLQPSPALSW RSTGGILDVY IFLGPEPKSV VRQYLDVVGY 370 380 390 400 410 420 PFMPPYWGLG FHLCRWGYSS TAITSQVVEN MTRAHFPLDV QWNDLDYMDA RRDFTFNKDG 430 440 450 460 470 480 FRDFPAMVRE LHQGGRRYMM IVDPAISSSG PAGSYRPYDE GLRRGVFITN ETSQPLIGKV 490 500 510 520 530 540 WPGSTAFPDF TNPAALAWWE DMVAEFHDQV PFDGMWIDMN EPSNFIRGSE DGCPDNELEN 550 560 570 580 590 600 PPYVPGVVGG TLQAATICAS SHQFLSTHYN LHNLYGLTEA IASHRALVKA RGTRPFVISR 610 620 630 640 650 660 STFAGHGRYA GHWTGDVWSS WEQLASSVPE ILQFNLLGVP LVGADVCGFL GNTSEELCVR 670 680 690 700 710 720 WTQLGAFYPF MRNHNGLLNL PQEPYSFSEP AQQAMRKALT LRYALLPHLY TLFHQAHVAG 730 740 750 760 770 780 ETVARPLFLE FPKDSSTWTV DHQLLWGEAL LITPVLQAGK AEVTGYFPLG IWYDLQTVPI 790 800 810 820 830 840 EALGSLPPPP AAPREPAIHS EGQWVTLPAP LDTINVHLRA GYIIPLQGPG LTTTESRQQP 850 860 870 880 890 900 MALAVALTKG GEARGELFWD DGESLEVLER GAYTQVLFLA RNNTIVNELV HVTSEGAGLQ 910 920 930 940 950 LQKVTVLGVA TTPQQVLSNG VPVSNFTYSP DTKVLDIPVS LLMAEQFLIS WS

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR860209 mRNA. Translation: CAH92351.1.
RefSeq	NP_001126384.1.
UniGene	Pab.6021
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH31. Glycoside Hydrolase Family 31.
Genome annotation databases
GeneID	100173365.
Organism-specific databases
CTD	100173365.
Phylogenomic databases
HOVERGEN	Q5R7A9.
Enzyme and pathway databases
BRENDA	3.2.1.20. 269192.
Family and domain databases
InterPro	IPR000322. Glyco_hydro_31. IPR000519. P_trefoil. IPR017957. P_trefoil_CS. [Graphical view]
PANTHER	PTHR22762. Glyco_hydro_31. 1 hit.
Pfam	PF01055. Glyco_hydro_31. 1 hit. PF00088. Trefoil. 1 hit. [Graphical view]
SMART	SM00018. PD. 1 hit. [Graphical view]
PROSITE	PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit. PS00707. GLYCOSYL_HYDROL_F31_2. 1 hit. PS00025. P_TREFOIL_1. 1 hit. PS51448. P_TREFOIL_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYAG_PONAB

Accession

Primary (citable) accession number: Q5R7A9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	December 21, 2004
Last modified:	September 1, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)