Q5R7A2 (ALG1_PONAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitobiosyldiphosphodolichol beta-mannosyltransferase
EC=2.4.1.142

Alternative name(s):
Asparagine-linked glycosylation protein 1 homolog
Beta-1,4-mannosyltransferase
GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase
GDP-mannose-dolichol diphosphochitobiose mannosyltransferase

Gene names

Name:

ALG1

Organism

Pongo abelii (Sumatran orangutan)

Taxonomic identifier

9601 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pongo

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man5 intermediate on the cytoplasmic surface of the ER By similarity.
Catalytic activity	GDP-mannose + chitobiosyldiphosphodolichol = GDP + beta-1,4-D-mannosylchitobiosyldiphosphodolichol.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase group 1 family. Glycosyltransferase 33 subfamily.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitobiosyldiphosphodolichol beta-mannosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Chitobiosyldiphosphodolichol beta-mannosyltransferase		PRO_0000080251
Regions
	Topological domain	1 – 2	2	Cytoplasmic Potential
	Transmembrane	3 – 23	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	24 – 464	441	Lumenal Potential
Amino acid modifications
	Modified residue	242	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5R7A2 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: 12CCBEC1ACA1BCA5	FASTA	464	52,505
	10 20 30 40 50 60 MAASCLVLLA LCLLLPLLLL GGWKRWRRGR TARHVVAVVL GDVGRSPRMQ YHALSLAMHG 70 80 90 100 110 120 FSVTLLGFCN SKPHDELLQN NRIQIVGLTE LQSLAVGPRV FQYGVKVVFQ AMYLLWKLMW 130 140 150 160 170 180 REPGAYIFLQ NPPGLPSIAV CWFVGCLCGS KLVIDWHNYG YSIMGLVHGP NHPLVLLAKW 190 200 210 220 230 240 YERFFGRLSH LNLCVTNAMR EDLAENWHIR AVTVYDKPAS FFKETPLDLQ HRLFMKLGGT 250 260 270 280 290 300 HSPFRARSEP EDPATERSAF TERDAGSGLV TRLHERPALL VSSTSWTEDE DFSILLAALE 310 320 330 340 350 360 KFEQLTLDGH SLPSLVCVIT GKGPLREYYS HLIHQKHFQH IQVCTPWLEA EDYPLLLGSA 370 380 390 400 410 420 DLGVCLHTSS SGLDLPMKVV DMFGCHLPVC AVNFKCLHEL VKHEENGLVF EDSEELAAQL 430 440 450 460 QMLFSNFPDP AGKLNQFRKN LRESQQLRWD ESWVQTVLPL VMDT

References

[1]	The German cDNA consortium Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR860216 mRNA. Translation: CAH92358.1.
RefSeq	NP_001126389.1. NM_001132917.1.
UniGene	Pab.9117.
3D structure databases
ProteinModelPortal	Q5R7A2.
ModBase	Search...
Protein family/group databases
CAZy	GT33. Glycosyltransferase Family 33.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSPPYT00000008333; ENSPPYP00000008002; ENSPPYG00000007077.
GeneID	100173370.
KEGG	pon:100173370.
Organism-specific databases
CTD	56052.
Phylogenomic databases
HOVERGEN	HBG055036.
InParanoid	Q5R7A2.
OMA	LQNPPGL.
OrthoDB	EOG49078X.
Family and domain databases
InterPro	IPR001296. Glyco_trans_1. [Graphical view]
KO	K03842.
Pfam	PF00534. Glycos_transf_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALG1_PONAB

Accession

Primary (citable) accession number: Q5R7A2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	December 21, 2004
Last modified:	November 16, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program