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Q5R739 (JOS1_PONAB) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Josephin-1

EC=3.4.19.12
Alternative name(s):
Josephin domain-containing protein 1
Gene names
Name:JOSD1
OrganismPongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii) [Reference proteome]
Taxonomic identifier9601 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaePongo

Protein attributes

Sequence length202 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Deubiquitinates monoubiquitinated probes (in vitro). When ubiquitinated, cleaves 'Lys-63'-linked and 'Lys-48'-linked poly-ubiquitin chains (in vitro), hence may act as a deubiquitinating enzyme. May increase macropinocytosis and suppress clathrin- and caveolae-mediated endocytosis. May enhance membrane dynamics and cell motility independently of its catalytic activity By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts with beta-actin/ACTB By similarity.

Subcellular location

Cell membrane By similarity. Cytoplasm By similarity. Note: Ubiquitination increases localization the plasma membrane By similarity. In the cytosol, the unubiquitinated form may be associated with the cytoskeleton via ACTB-binding By similarity.

Post-translational modification

Monoubiquitinated By similarity. Ubiquitination activates deubiquitination activity in vitro By similarity.

Sequence similarities

Contains 1 Josephin domain.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Cellular componentCell membrane
Cytoplasm
Membrane
   Molecular functionHydrolase
Protease
   PTMUbl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionomega peptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 202202Josephin-1
PRO_0000053841

Regions

Domain23 – 202180Josephin

Sites

Active site361Nucleophile By similarity
Active site1391Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5R739 [UniParc].

Last modified December 21, 2004. Version 1.
Checksum: 837268C393C0A5A9

FASTA20223,198
        10         20         30         40         50         60 
MSCVPWKGDK AKSESLELPQ AAPPQIYHEK QRRELCALHA LNNVFQDSNA FTRDTLQEIF 

        70         80         90        100        110        120 
QRLSPNTMVT PHKKSMLGNG NYDVNVIMAA LQTKGYEAVW WDKRRDVGVI ALTNVMGFIM 

       130        140        150        160        170        180 
NLPSSLCWGP LKLPLKRQHW ICVREVGGAY YNLDSKLKMP EWIGGESELR KFLKHHLRGK 

       190        200 
NCELLLVVPE EVEAHQSWRT DV 

« Hide

References

[1]The German cDNA consortium
Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain cortex.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR860279 mRNA. Translation: CAH92421.1.
RefSeqNP_001126428.1. NM_001132956.1.

3D structure databases

ProteinModelPortalQ5R739.
SMRQ5R739. Positions 24-196.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC86.004.

Proteomic databases

PRIDEQ5R739.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100173411.
KEGGpon:100173411.

Organism-specific databases

CTD9929.

Phylogenomic databases

HOVERGENHBG039523.
InParanoidQ5R739.
KOK15235.

Family and domain databases

InterProIPR006155. Josephin.
[Graphical view]
PfamPF02099. Josephin. 1 hit.
[Graphical view]
PROSITEPS50957. JOSEPHIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameJOS1_PONAB
AccessionPrimary (citable) accession number: Q5R739
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 21, 2004
Last modified: April 16, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families